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Matrix Metalloproteinases System and Types of Fibrosis in Rat Heart during Late Pregnancy and Postpartum

Background and objectives: Cardiac remodeling in pregnancy and postpartum is poorly understood. The aim of this study was to evaluate changes in cardiac fibrosis (pericardial, perivascular, and interstitial), as well as the expression of matrix metalloproteinases (MMP-1, MMP-2, and MMP-9) and their...

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Autores principales: Virgen-Ortiz, Adolfo, Limón-Miranda, Saraí, Salazar-Enríquez, Diana Guadalupe, Melnikov, Valery, Sánchez-Pastor, Enrique Alejandro, Castro-Rodríguez, Elena Margarita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571987/
https://www.ncbi.nlm.nih.gov/pubmed/31126142
http://dx.doi.org/10.3390/medicina55050199
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author Virgen-Ortiz, Adolfo
Limón-Miranda, Saraí
Salazar-Enríquez, Diana Guadalupe
Melnikov, Valery
Sánchez-Pastor, Enrique Alejandro
Castro-Rodríguez, Elena Margarita
author_facet Virgen-Ortiz, Adolfo
Limón-Miranda, Saraí
Salazar-Enríquez, Diana Guadalupe
Melnikov, Valery
Sánchez-Pastor, Enrique Alejandro
Castro-Rodríguez, Elena Margarita
author_sort Virgen-Ortiz, Adolfo
collection PubMed
description Background and objectives: Cardiac remodeling in pregnancy and postpartum is poorly understood. The aim of this study was to evaluate changes in cardiac fibrosis (pericardial, perivascular, and interstitial), as well as the expression of matrix metalloproteinases (MMP-1, MMP-2, and MMP-9) and their inhibitors (Tissue inhibitors of metalloproteinases, TIMP-1 and TIMP-4) during late pregnancy and postpartum in rat left ventricle. Materials and Methods: Female Sprague–Dawley rats were used for this study. Rats were divided three groups: non-pregnant, late pregnancy, and postpartum. The heart was weighed and cardiac fibrosis was studied by conventional histological procedures. The expression and transcript level of target proteins were evaluated using immunoblot techniques and quantitative PCR. Results: The experiments showed an increase of perivascular, pericardial, and interstitial fibrosis in heart during pregnancy and its reversion in postpartum. Moreover, in late pregnancy, MMP-1, MMP-2, and MMP-9 metalloproteinases were downregulated and TIMP-1 and TIMP-4 were upregulated in left ventricle. Conclusions: Our data suggest that the metalloproteinases system is involved in the cardiac extracellular matrix remodeling during pregnancy and its reversion in postpartum, this improves the knowledge of the adaptive cardiac remodeling in response to a blood volume overload present during pregnancy.
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spelling pubmed-65719872019-06-18 Matrix Metalloproteinases System and Types of Fibrosis in Rat Heart during Late Pregnancy and Postpartum Virgen-Ortiz, Adolfo Limón-Miranda, Saraí Salazar-Enríquez, Diana Guadalupe Melnikov, Valery Sánchez-Pastor, Enrique Alejandro Castro-Rodríguez, Elena Margarita Medicina (Kaunas) Article Background and objectives: Cardiac remodeling in pregnancy and postpartum is poorly understood. The aim of this study was to evaluate changes in cardiac fibrosis (pericardial, perivascular, and interstitial), as well as the expression of matrix metalloproteinases (MMP-1, MMP-2, and MMP-9) and their inhibitors (Tissue inhibitors of metalloproteinases, TIMP-1 and TIMP-4) during late pregnancy and postpartum in rat left ventricle. Materials and Methods: Female Sprague–Dawley rats were used for this study. Rats were divided three groups: non-pregnant, late pregnancy, and postpartum. The heart was weighed and cardiac fibrosis was studied by conventional histological procedures. The expression and transcript level of target proteins were evaluated using immunoblot techniques and quantitative PCR. Results: The experiments showed an increase of perivascular, pericardial, and interstitial fibrosis in heart during pregnancy and its reversion in postpartum. Moreover, in late pregnancy, MMP-1, MMP-2, and MMP-9 metalloproteinases were downregulated and TIMP-1 and TIMP-4 were upregulated in left ventricle. Conclusions: Our data suggest that the metalloproteinases system is involved in the cardiac extracellular matrix remodeling during pregnancy and its reversion in postpartum, this improves the knowledge of the adaptive cardiac remodeling in response to a blood volume overload present during pregnancy. MDPI 2019-05-23 /pmc/articles/PMC6571987/ /pubmed/31126142 http://dx.doi.org/10.3390/medicina55050199 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Virgen-Ortiz, Adolfo
Limón-Miranda, Saraí
Salazar-Enríquez, Diana Guadalupe
Melnikov, Valery
Sánchez-Pastor, Enrique Alejandro
Castro-Rodríguez, Elena Margarita
Matrix Metalloproteinases System and Types of Fibrosis in Rat Heart during Late Pregnancy and Postpartum
title Matrix Metalloproteinases System and Types of Fibrosis in Rat Heart during Late Pregnancy and Postpartum
title_full Matrix Metalloproteinases System and Types of Fibrosis in Rat Heart during Late Pregnancy and Postpartum
title_fullStr Matrix Metalloproteinases System and Types of Fibrosis in Rat Heart during Late Pregnancy and Postpartum
title_full_unstemmed Matrix Metalloproteinases System and Types of Fibrosis in Rat Heart during Late Pregnancy and Postpartum
title_short Matrix Metalloproteinases System and Types of Fibrosis in Rat Heart during Late Pregnancy and Postpartum
title_sort matrix metalloproteinases system and types of fibrosis in rat heart during late pregnancy and postpartum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571987/
https://www.ncbi.nlm.nih.gov/pubmed/31126142
http://dx.doi.org/10.3390/medicina55050199
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