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Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns

Lipid-based membranes play crucial roles in regulating the interface between cells and their external environment, the communication within cells, and cellular sensing. To study these important processes, various lipid-based artificial membrane models have been developed in recent years and, indeed,...

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Detalles Bibliográficos
Autores principales: Angelin, Alessandro, Bog, Uwe, Kumar, Ravi, Niemeyer, Christof M., Hirtz, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572014/
https://www.ncbi.nlm.nih.gov/pubmed/31096642
http://dx.doi.org/10.3390/polym11050891
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author Angelin, Alessandro
Bog, Uwe
Kumar, Ravi
Niemeyer, Christof M.
Hirtz, Michael
author_facet Angelin, Alessandro
Bog, Uwe
Kumar, Ravi
Niemeyer, Christof M.
Hirtz, Michael
author_sort Angelin, Alessandro
collection PubMed
description Lipid-based membranes play crucial roles in regulating the interface between cells and their external environment, the communication within cells, and cellular sensing. To study these important processes, various lipid-based artificial membrane models have been developed in recent years and, indeed, large-area arrays of supported lipid bilayers suit the needs of many of these studies remarkably well. Here, the direct-write scanning probe lithography technique called polymer pen lithography (PPL) was used as a tool for the creation of lipid micropatterns over large areas via polymer-stamp-mediated transfer of lipid-containing inks onto glass substrates. In order to better understand and control the lipid transfer in PPL, we conducted a systematic study of the influence of dwell time (i.e., duration of contact between tip and sample), humidity, and printing pressure on the outcome of PPL with phospholipids and discuss results in comparison to the more often studied dip-pen nanolithography with phospholipids. This is the first systematic study in phospholipid printing with PPL. Biocompatibility of the obtained substrates with up to two different ink compositions was demonstrated. The patterns are suitable to serve as a platform for mast cell activation experiments.
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spelling pubmed-65720142019-06-18 Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns Angelin, Alessandro Bog, Uwe Kumar, Ravi Niemeyer, Christof M. Hirtz, Michael Polymers (Basel) Article Lipid-based membranes play crucial roles in regulating the interface between cells and their external environment, the communication within cells, and cellular sensing. To study these important processes, various lipid-based artificial membrane models have been developed in recent years and, indeed, large-area arrays of supported lipid bilayers suit the needs of many of these studies remarkably well. Here, the direct-write scanning probe lithography technique called polymer pen lithography (PPL) was used as a tool for the creation of lipid micropatterns over large areas via polymer-stamp-mediated transfer of lipid-containing inks onto glass substrates. In order to better understand and control the lipid transfer in PPL, we conducted a systematic study of the influence of dwell time (i.e., duration of contact between tip and sample), humidity, and printing pressure on the outcome of PPL with phospholipids and discuss results in comparison to the more often studied dip-pen nanolithography with phospholipids. This is the first systematic study in phospholipid printing with PPL. Biocompatibility of the obtained substrates with up to two different ink compositions was demonstrated. The patterns are suitable to serve as a platform for mast cell activation experiments. MDPI 2019-05-15 /pmc/articles/PMC6572014/ /pubmed/31096642 http://dx.doi.org/10.3390/polym11050891 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Angelin, Alessandro
Bog, Uwe
Kumar, Ravi
Niemeyer, Christof M.
Hirtz, Michael
Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns
title Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns
title_full Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns
title_fullStr Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns
title_full_unstemmed Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns
title_short Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns
title_sort writing behavior of phospholipids in polymer pen lithography (ppl) for bioactive micropatterns
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572014/
https://www.ncbi.nlm.nih.gov/pubmed/31096642
http://dx.doi.org/10.3390/polym11050891
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