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UHPLC-UV/Vis Quantitative Analysis of Hydroxylated and O-prenylated Coumarins in Pomegranate Seed Extracts

A simple and rapid analytical UHPLC methodology with spectrophotometric (UV/Vis) detection, coupled with different extraction procedures, has been perfected to investigate the presence of biologically active O-prenylated umbelliferone derivatives, such as auraptene and umbelliprenin, in pomegranate...

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Autores principales: Fiorito, Serena, Ianni, Federica, Preziuso, Francesca, Epifano, Francesco, Scotti, Luca, Bucciarelli, Tonino, Genovese, Salvatore
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572463/
https://www.ncbi.nlm.nih.gov/pubmed/31121819
http://dx.doi.org/10.3390/molecules24101963
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author Fiorito, Serena
Ianni, Federica
Preziuso, Francesca
Epifano, Francesco
Scotti, Luca
Bucciarelli, Tonino
Genovese, Salvatore
author_facet Fiorito, Serena
Ianni, Federica
Preziuso, Francesca
Epifano, Francesco
Scotti, Luca
Bucciarelli, Tonino
Genovese, Salvatore
author_sort Fiorito, Serena
collection PubMed
description A simple and rapid analytical UHPLC methodology with spectrophotometric (UV/Vis) detection, coupled with different extraction procedures, has been perfected to investigate the presence of biologically active O-prenylated umbelliferone derivatives, such as auraptene and umbelliprenin, in pomegranate (Punica granatum L.) seed extracts. Absolute ethanol was the most efficient extraction solvent in terms of yields, after a short ultrasound-assisted. The highest concentration values recorded under these experimental conditions were 1.99 μg/g of dry extract and 6.53 μg/g for auraptene and umbelliprenin, respectively. The parent metabolite umbelliferone was also detected (0.67 μg/g). The extraction and UHPLC analytical methodology set up in the present study proved to be an efficient, powerful, and versatile technique for the simultaneous qualitative analysis and quantification of oxyprenylated coumarins in pomegranate seed extracts. The characterization of such secondary metabolites in the mentioned phytopreparation represents, to the best of our knowledge, the first example in the literature.
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spelling pubmed-65724632019-06-18 UHPLC-UV/Vis Quantitative Analysis of Hydroxylated and O-prenylated Coumarins in Pomegranate Seed Extracts Fiorito, Serena Ianni, Federica Preziuso, Francesca Epifano, Francesco Scotti, Luca Bucciarelli, Tonino Genovese, Salvatore Molecules Communication A simple and rapid analytical UHPLC methodology with spectrophotometric (UV/Vis) detection, coupled with different extraction procedures, has been perfected to investigate the presence of biologically active O-prenylated umbelliferone derivatives, such as auraptene and umbelliprenin, in pomegranate (Punica granatum L.) seed extracts. Absolute ethanol was the most efficient extraction solvent in terms of yields, after a short ultrasound-assisted. The highest concentration values recorded under these experimental conditions were 1.99 μg/g of dry extract and 6.53 μg/g for auraptene and umbelliprenin, respectively. The parent metabolite umbelliferone was also detected (0.67 μg/g). The extraction and UHPLC analytical methodology set up in the present study proved to be an efficient, powerful, and versatile technique for the simultaneous qualitative analysis and quantification of oxyprenylated coumarins in pomegranate seed extracts. The characterization of such secondary metabolites in the mentioned phytopreparation represents, to the best of our knowledge, the first example in the literature. MDPI 2019-05-22 /pmc/articles/PMC6572463/ /pubmed/31121819 http://dx.doi.org/10.3390/molecules24101963 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Fiorito, Serena
Ianni, Federica
Preziuso, Francesca
Epifano, Francesco
Scotti, Luca
Bucciarelli, Tonino
Genovese, Salvatore
UHPLC-UV/Vis Quantitative Analysis of Hydroxylated and O-prenylated Coumarins in Pomegranate Seed Extracts
title UHPLC-UV/Vis Quantitative Analysis of Hydroxylated and O-prenylated Coumarins in Pomegranate Seed Extracts
title_full UHPLC-UV/Vis Quantitative Analysis of Hydroxylated and O-prenylated Coumarins in Pomegranate Seed Extracts
title_fullStr UHPLC-UV/Vis Quantitative Analysis of Hydroxylated and O-prenylated Coumarins in Pomegranate Seed Extracts
title_full_unstemmed UHPLC-UV/Vis Quantitative Analysis of Hydroxylated and O-prenylated Coumarins in Pomegranate Seed Extracts
title_short UHPLC-UV/Vis Quantitative Analysis of Hydroxylated and O-prenylated Coumarins in Pomegranate Seed Extracts
title_sort uhplc-uv/vis quantitative analysis of hydroxylated and o-prenylated coumarins in pomegranate seed extracts
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572463/
https://www.ncbi.nlm.nih.gov/pubmed/31121819
http://dx.doi.org/10.3390/molecules24101963
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