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Mutation Breeding in Tomato: Advances, Applicability and Challenges

Induced mutagenesis is one of the most effective strategies for trait improvement without altering the well-optimized genetic background of the cultivars. In this review, several currently accessible methods such as physical, chemical and insertional mutagenesis have been discussed concerning their...

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Autores principales: Chaudhary, Juhi, Alisha, Alisha, Bhatt, Vacha, Chandanshive, Sonali, Kumar, Nirbhay, Mir, Zahoor, Kumar, Ashwini, Yadav, Satish K., Shivaraj, S. M., Sonah, Humira, Deshmukh, Rupesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572636/
https://www.ncbi.nlm.nih.gov/pubmed/31091747
http://dx.doi.org/10.3390/plants8050128
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author Chaudhary, Juhi
Alisha, Alisha
Bhatt, Vacha
Chandanshive, Sonali
Kumar, Nirbhay
Mir, Zahoor
Kumar, Ashwini
Yadav, Satish K.
Shivaraj, S. M.
Sonah, Humira
Deshmukh, Rupesh
author_facet Chaudhary, Juhi
Alisha, Alisha
Bhatt, Vacha
Chandanshive, Sonali
Kumar, Nirbhay
Mir, Zahoor
Kumar, Ashwini
Yadav, Satish K.
Shivaraj, S. M.
Sonah, Humira
Deshmukh, Rupesh
author_sort Chaudhary, Juhi
collection PubMed
description Induced mutagenesis is one of the most effective strategies for trait improvement without altering the well-optimized genetic background of the cultivars. In this review, several currently accessible methods such as physical, chemical and insertional mutagenesis have been discussed concerning their efficient exploration for the tomato crop improvement. Similarly, challenges for the adaptation of genome-editing, a newly developed technique providing an opportunity to induce precise mutation, have been addressed. Several efforts of genome-editing have been demonstrated in tomato and other crops, exploring its effectiveness and convenience for crop improvement. Descriptive data compiled here from such efforts will be helpful for the efficient exploration of technological advances. However, uncertainty about the regulation of genome-edited crops is still a significant concern, particularly when timely trait improvement in tomato cultivars is needed. In this regard, random approaches of induced mutagenesis are still promising if efficiently explored in breeding applications. Precise identification of casual mutation is a prerequisite for the molecular understanding of the trait development as well as its utilization for the breeding program. Recent advances in sequencing techniques provide an opportunity for the precise detection of mutagenesis-induced sequence variations at a large scale in the genome. Here, we reviewed several novel next-generation sequencing based mutation mapping approaches including Mutmap, MutChromeSeq, and whole-genome sequencing-based mapping which has enormous potential to accelerate the mutation breeding in tomato. The proper utilization of the existing well-characterized tomato mutant resources combined with novel mapping approaches would inevitably lead to rapid enhancement of tomato quality and yield. This article provides an overview of the principles and applications of mutagenesis approaches in tomato and discusses the current progress and challenges involved in tomato mutagenesis research.
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spelling pubmed-65726362019-06-18 Mutation Breeding in Tomato: Advances, Applicability and Challenges Chaudhary, Juhi Alisha, Alisha Bhatt, Vacha Chandanshive, Sonali Kumar, Nirbhay Mir, Zahoor Kumar, Ashwini Yadav, Satish K. Shivaraj, S. M. Sonah, Humira Deshmukh, Rupesh Plants (Basel) Review Induced mutagenesis is one of the most effective strategies for trait improvement without altering the well-optimized genetic background of the cultivars. In this review, several currently accessible methods such as physical, chemical and insertional mutagenesis have been discussed concerning their efficient exploration for the tomato crop improvement. Similarly, challenges for the adaptation of genome-editing, a newly developed technique providing an opportunity to induce precise mutation, have been addressed. Several efforts of genome-editing have been demonstrated in tomato and other crops, exploring its effectiveness and convenience for crop improvement. Descriptive data compiled here from such efforts will be helpful for the efficient exploration of technological advances. However, uncertainty about the regulation of genome-edited crops is still a significant concern, particularly when timely trait improvement in tomato cultivars is needed. In this regard, random approaches of induced mutagenesis are still promising if efficiently explored in breeding applications. Precise identification of casual mutation is a prerequisite for the molecular understanding of the trait development as well as its utilization for the breeding program. Recent advances in sequencing techniques provide an opportunity for the precise detection of mutagenesis-induced sequence variations at a large scale in the genome. Here, we reviewed several novel next-generation sequencing based mutation mapping approaches including Mutmap, MutChromeSeq, and whole-genome sequencing-based mapping which has enormous potential to accelerate the mutation breeding in tomato. The proper utilization of the existing well-characterized tomato mutant resources combined with novel mapping approaches would inevitably lead to rapid enhancement of tomato quality and yield. This article provides an overview of the principles and applications of mutagenesis approaches in tomato and discusses the current progress and challenges involved in tomato mutagenesis research. MDPI 2019-05-14 /pmc/articles/PMC6572636/ /pubmed/31091747 http://dx.doi.org/10.3390/plants8050128 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Chaudhary, Juhi
Alisha, Alisha
Bhatt, Vacha
Chandanshive, Sonali
Kumar, Nirbhay
Mir, Zahoor
Kumar, Ashwini
Yadav, Satish K.
Shivaraj, S. M.
Sonah, Humira
Deshmukh, Rupesh
Mutation Breeding in Tomato: Advances, Applicability and Challenges
title Mutation Breeding in Tomato: Advances, Applicability and Challenges
title_full Mutation Breeding in Tomato: Advances, Applicability and Challenges
title_fullStr Mutation Breeding in Tomato: Advances, Applicability and Challenges
title_full_unstemmed Mutation Breeding in Tomato: Advances, Applicability and Challenges
title_short Mutation Breeding in Tomato: Advances, Applicability and Challenges
title_sort mutation breeding in tomato: advances, applicability and challenges
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572636/
https://www.ncbi.nlm.nih.gov/pubmed/31091747
http://dx.doi.org/10.3390/plants8050128
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