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Polyhexamethylene Biguanide:Polyurethane Blend Nanofibrous Membranes for Wound Infection Control

Polyhexamethylene biguanide (PHMB) is a broad-spectrum antiseptic which avoids many efficacy and toxicity problems associated with antimicrobials, in particular, it has a low risk of loss of susceptibility due to acquired antimicrobial resistance. Despite such advantages, PHMB is not widely used in...

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Autores principales: Worsley, Anna, Vassileva, Kristin, Tsui, Janice, Song, Wenhui, Good, Liam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572704/
https://www.ncbi.nlm.nih.gov/pubmed/31121845
http://dx.doi.org/10.3390/polym11050915
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author Worsley, Anna
Vassileva, Kristin
Tsui, Janice
Song, Wenhui
Good, Liam
author_facet Worsley, Anna
Vassileva, Kristin
Tsui, Janice
Song, Wenhui
Good, Liam
author_sort Worsley, Anna
collection PubMed
description Polyhexamethylene biguanide (PHMB) is a broad-spectrum antiseptic which avoids many efficacy and toxicity problems associated with antimicrobials, in particular, it has a low risk of loss of susceptibility due to acquired antimicrobial resistance. Despite such advantages, PHMB is not widely used in wound care, suggesting more research is required to take full advantage of PHMB’s properties. We hypothesised that a nanofibre morphology would provide a gradual release of PHMB, prolonging the antimicrobial effects within the therapeutic window. PHMB:polyurethane (PU) electrospun nanofibre membranes were prepared with increasing PHMB concentrations, and the effects on antimicrobial activities, mechanical properties and host cell toxicity were compared. Overall, PHMB:PU membranes displayed a burst release of PHMB during the first hour following PBS immersion (50.5–95.9% of total released), followed by a gradual release over 120 h (≤25 wt % PHMB). The membranes were hydrophilic (83.7–53.3°), gradually gaining hydrophobicity as PHMB was released. They displayed superior antimicrobial activity, which extended past the initial release period, retained PU hyperelasticity regardless of PHMB concentration (collective tensile modulus of 5–35% PHMB:PU membranes, 3.56 ± 0.97 MPa; ultimate strain, >200%) and displayed minimal human cell toxicity (<25 wt % PHMB). With further development, PHMB:PU electrospun membranes may provide improved wound dressings.
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spelling pubmed-65727042019-06-18 Polyhexamethylene Biguanide:Polyurethane Blend Nanofibrous Membranes for Wound Infection Control Worsley, Anna Vassileva, Kristin Tsui, Janice Song, Wenhui Good, Liam Polymers (Basel) Article Polyhexamethylene biguanide (PHMB) is a broad-spectrum antiseptic which avoids many efficacy and toxicity problems associated with antimicrobials, in particular, it has a low risk of loss of susceptibility due to acquired antimicrobial resistance. Despite such advantages, PHMB is not widely used in wound care, suggesting more research is required to take full advantage of PHMB’s properties. We hypothesised that a nanofibre morphology would provide a gradual release of PHMB, prolonging the antimicrobial effects within the therapeutic window. PHMB:polyurethane (PU) electrospun nanofibre membranes were prepared with increasing PHMB concentrations, and the effects on antimicrobial activities, mechanical properties and host cell toxicity were compared. Overall, PHMB:PU membranes displayed a burst release of PHMB during the first hour following PBS immersion (50.5–95.9% of total released), followed by a gradual release over 120 h (≤25 wt % PHMB). The membranes were hydrophilic (83.7–53.3°), gradually gaining hydrophobicity as PHMB was released. They displayed superior antimicrobial activity, which extended past the initial release period, retained PU hyperelasticity regardless of PHMB concentration (collective tensile modulus of 5–35% PHMB:PU membranes, 3.56 ± 0.97 MPa; ultimate strain, >200%) and displayed minimal human cell toxicity (<25 wt % PHMB). With further development, PHMB:PU electrospun membranes may provide improved wound dressings. MDPI 2019-05-22 /pmc/articles/PMC6572704/ /pubmed/31121845 http://dx.doi.org/10.3390/polym11050915 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Worsley, Anna
Vassileva, Kristin
Tsui, Janice
Song, Wenhui
Good, Liam
Polyhexamethylene Biguanide:Polyurethane Blend Nanofibrous Membranes for Wound Infection Control
title Polyhexamethylene Biguanide:Polyurethane Blend Nanofibrous Membranes for Wound Infection Control
title_full Polyhexamethylene Biguanide:Polyurethane Blend Nanofibrous Membranes for Wound Infection Control
title_fullStr Polyhexamethylene Biguanide:Polyurethane Blend Nanofibrous Membranes for Wound Infection Control
title_full_unstemmed Polyhexamethylene Biguanide:Polyurethane Blend Nanofibrous Membranes for Wound Infection Control
title_short Polyhexamethylene Biguanide:Polyurethane Blend Nanofibrous Membranes for Wound Infection Control
title_sort polyhexamethylene biguanide:polyurethane blend nanofibrous membranes for wound infection control
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572704/
https://www.ncbi.nlm.nih.gov/pubmed/31121845
http://dx.doi.org/10.3390/polym11050915
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