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Adipose Tissue Mast Cells Promote Human Adipose Beiging in Response to Cold
In a recent study, repeated cold application induced beiging in subcutaneous white adipose tissue (SC WAT) of humans independent of body mass index. To identify factors that promote or inhibit beiging, we performed multiplex analysis of gene expression with the Nanostring nCounter system (the probe...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572779/ https://www.ncbi.nlm.nih.gov/pubmed/31209239 http://dx.doi.org/10.1038/s41598-019-45136-9 |
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author | Finlin, Brian S. Confides, Amy L. Zhu, Beibei Boulanger, Mary C. Memetimin, Hasiyet Taylor, Kyle W. Johnson, Zachary R. Westgate, Philip M. Dupont-Versteegden, Esther E. Kern, Philip A. |
author_facet | Finlin, Brian S. Confides, Amy L. Zhu, Beibei Boulanger, Mary C. Memetimin, Hasiyet Taylor, Kyle W. Johnson, Zachary R. Westgate, Philip M. Dupont-Versteegden, Esther E. Kern, Philip A. |
author_sort | Finlin, Brian S. |
collection | PubMed |
description | In a recent study, repeated cold application induced beiging in subcutaneous white adipose tissue (SC WAT) of humans independent of body mass index. To identify factors that promote or inhibit beiging, we performed multiplex analysis of gene expression with the Nanostring nCounter system (the probe set contained genes for specific immune cell markers, cytokines, and chemokines) on the SC WAT from lean subjects. Multiple correlations analysis identified mast cell tryptase and CCL26, a chemokine for mast cells, as genes whose change correlated positively with the change in UCP1 in SC WAT, leading to the hypothesis that mast cells promote SC WAT beiging in response to cold. We quantified mast cell recruitment into SC WAT and degranulation. Mast cells increased in number in SC WAT in lean subjects, and there was an increase in the number of degranulated mast cells in both lean subjects and subjects with obesity. We determined that norepinephrine stimulated mast cell degranulation and histamine release in vitro. In conclusion, cold stimulated adipose tissue mast cell recruitment in lean subjects and mast cell degranulation in SC WAT of all research participants independent of baseline body mass index, suggesting that mast cells promote adipose beiging through the release of histamine or other products. |
format | Online Article Text |
id | pubmed-6572779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65727792019-06-24 Adipose Tissue Mast Cells Promote Human Adipose Beiging in Response to Cold Finlin, Brian S. Confides, Amy L. Zhu, Beibei Boulanger, Mary C. Memetimin, Hasiyet Taylor, Kyle W. Johnson, Zachary R. Westgate, Philip M. Dupont-Versteegden, Esther E. Kern, Philip A. Sci Rep Article In a recent study, repeated cold application induced beiging in subcutaneous white adipose tissue (SC WAT) of humans independent of body mass index. To identify factors that promote or inhibit beiging, we performed multiplex analysis of gene expression with the Nanostring nCounter system (the probe set contained genes for specific immune cell markers, cytokines, and chemokines) on the SC WAT from lean subjects. Multiple correlations analysis identified mast cell tryptase and CCL26, a chemokine for mast cells, as genes whose change correlated positively with the change in UCP1 in SC WAT, leading to the hypothesis that mast cells promote SC WAT beiging in response to cold. We quantified mast cell recruitment into SC WAT and degranulation. Mast cells increased in number in SC WAT in lean subjects, and there was an increase in the number of degranulated mast cells in both lean subjects and subjects with obesity. We determined that norepinephrine stimulated mast cell degranulation and histamine release in vitro. In conclusion, cold stimulated adipose tissue mast cell recruitment in lean subjects and mast cell degranulation in SC WAT of all research participants independent of baseline body mass index, suggesting that mast cells promote adipose beiging through the release of histamine or other products. Nature Publishing Group UK 2019-06-17 /pmc/articles/PMC6572779/ /pubmed/31209239 http://dx.doi.org/10.1038/s41598-019-45136-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Finlin, Brian S. Confides, Amy L. Zhu, Beibei Boulanger, Mary C. Memetimin, Hasiyet Taylor, Kyle W. Johnson, Zachary R. Westgate, Philip M. Dupont-Versteegden, Esther E. Kern, Philip A. Adipose Tissue Mast Cells Promote Human Adipose Beiging in Response to Cold |
title | Adipose Tissue Mast Cells Promote Human Adipose Beiging in Response to Cold |
title_full | Adipose Tissue Mast Cells Promote Human Adipose Beiging in Response to Cold |
title_fullStr | Adipose Tissue Mast Cells Promote Human Adipose Beiging in Response to Cold |
title_full_unstemmed | Adipose Tissue Mast Cells Promote Human Adipose Beiging in Response to Cold |
title_short | Adipose Tissue Mast Cells Promote Human Adipose Beiging in Response to Cold |
title_sort | adipose tissue mast cells promote human adipose beiging in response to cold |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572779/ https://www.ncbi.nlm.nih.gov/pubmed/31209239 http://dx.doi.org/10.1038/s41598-019-45136-9 |
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