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Molecular Characterization and Gene Expression of Glutathione Peroxidase 1 in Tor tambroides Exposed to Temperature Stress

Temperature is an abiotic factor that affects various biological and physiological processes in fish. Temperature stress is known to increase the production of reactive oxygen species (ROS) that subsequently cause oxidative stress. Fish is known to evolve a system of antioxidant enzymes to reduce RO...

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Autores principales: Do, Thinh Dinh, Thi Mai, Nguyen, Duy Khoa, Tran Nguyen, Abol-Munafi, Ambok Bolong, Liew, Hon Jung, Kim, Chang-Bae, Wong, Li Lian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572904/
https://www.ncbi.nlm.nih.gov/pubmed/31236006
http://dx.doi.org/10.1177/1176934319853580
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author Do, Thinh Dinh
Thi Mai, Nguyen
Duy Khoa, Tran Nguyen
Abol-Munafi, Ambok Bolong
Liew, Hon Jung
Kim, Chang-Bae
Wong, Li Lian
author_facet Do, Thinh Dinh
Thi Mai, Nguyen
Duy Khoa, Tran Nguyen
Abol-Munafi, Ambok Bolong
Liew, Hon Jung
Kim, Chang-Bae
Wong, Li Lian
author_sort Do, Thinh Dinh
collection PubMed
description Temperature is an abiotic factor that affects various biological and physiological processes in fish. Temperature stress is known to increase the production of reactive oxygen species (ROS) that subsequently cause oxidative stress. Fish is known to evolve a system of antioxidant enzymes to reduce ROS toxicology. Glutathione peroxidase (GPx) family consists of key enzymes that protect fish from oxidative stress. In this study, full-length GPx1 cDNA (GenBank accession no. KY984468) of Tor tambroides was cloned and characterized by rapid amplification of cDNA ends (RACE). The 899-base-pair (bp) GPx1 cDNA includes a 576-bp open reading frame encoding for 191 amino acids, plus 28 bp of 5′-untranslated region (UTR) and 295 bp of 3′-UTR. Homology analysis revealed that GPx1 of T tambroides (Tor-GPx1) shared high similarity with GPx1 sequences of other fish species. The phylogenetic construction based on the amino acid sequence showed that Tor-GPx1 formed a clade with GPx1 sequences of various fish species. Real-time polymerase chain reaction (PCR) was performed to assess the levels of GPx1 gene expression in the liver and muscle of T tambroides under thermal stress. The results indicated that GPx1 gene expression was down-regulated under decreased temperature. However, there was no significant difference between GPx1 gene expression in fish exposed to high temperature and control. Our study provides the first data regarding GPx gene expression in T tambroides under thermal stress.
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spelling pubmed-65729042019-06-24 Molecular Characterization and Gene Expression of Glutathione Peroxidase 1 in Tor tambroides Exposed to Temperature Stress Do, Thinh Dinh Thi Mai, Nguyen Duy Khoa, Tran Nguyen Abol-Munafi, Ambok Bolong Liew, Hon Jung Kim, Chang-Bae Wong, Li Lian Evol Bioinform Online Molecular Diversity and Conservation Temperature is an abiotic factor that affects various biological and physiological processes in fish. Temperature stress is known to increase the production of reactive oxygen species (ROS) that subsequently cause oxidative stress. Fish is known to evolve a system of antioxidant enzymes to reduce ROS toxicology. Glutathione peroxidase (GPx) family consists of key enzymes that protect fish from oxidative stress. In this study, full-length GPx1 cDNA (GenBank accession no. KY984468) of Tor tambroides was cloned and characterized by rapid amplification of cDNA ends (RACE). The 899-base-pair (bp) GPx1 cDNA includes a 576-bp open reading frame encoding for 191 amino acids, plus 28 bp of 5′-untranslated region (UTR) and 295 bp of 3′-UTR. Homology analysis revealed that GPx1 of T tambroides (Tor-GPx1) shared high similarity with GPx1 sequences of other fish species. The phylogenetic construction based on the amino acid sequence showed that Tor-GPx1 formed a clade with GPx1 sequences of various fish species. Real-time polymerase chain reaction (PCR) was performed to assess the levels of GPx1 gene expression in the liver and muscle of T tambroides under thermal stress. The results indicated that GPx1 gene expression was down-regulated under decreased temperature. However, there was no significant difference between GPx1 gene expression in fish exposed to high temperature and control. Our study provides the first data regarding GPx gene expression in T tambroides under thermal stress. SAGE Publications 2019-06-13 /pmc/articles/PMC6572904/ /pubmed/31236006 http://dx.doi.org/10.1177/1176934319853580 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www.creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Molecular Diversity and Conservation
Do, Thinh Dinh
Thi Mai, Nguyen
Duy Khoa, Tran Nguyen
Abol-Munafi, Ambok Bolong
Liew, Hon Jung
Kim, Chang-Bae
Wong, Li Lian
Molecular Characterization and Gene Expression of Glutathione Peroxidase 1 in Tor tambroides Exposed to Temperature Stress
title Molecular Characterization and Gene Expression of Glutathione Peroxidase 1 in Tor tambroides Exposed to Temperature Stress
title_full Molecular Characterization and Gene Expression of Glutathione Peroxidase 1 in Tor tambroides Exposed to Temperature Stress
title_fullStr Molecular Characterization and Gene Expression of Glutathione Peroxidase 1 in Tor tambroides Exposed to Temperature Stress
title_full_unstemmed Molecular Characterization and Gene Expression of Glutathione Peroxidase 1 in Tor tambroides Exposed to Temperature Stress
title_short Molecular Characterization and Gene Expression of Glutathione Peroxidase 1 in Tor tambroides Exposed to Temperature Stress
title_sort molecular characterization and gene expression of glutathione peroxidase 1 in tor tambroides exposed to temperature stress
topic Molecular Diversity and Conservation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572904/
https://www.ncbi.nlm.nih.gov/pubmed/31236006
http://dx.doi.org/10.1177/1176934319853580
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