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Natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the PAPCLEAR cohort study

INTRODUCTION: Human papillomaviruses (HPVs) are responsible for one-third of all cancers caused by infections. Most HPV studies focus on chronic infections and cancers, and we know little about the early stages of the infection. Our main objective is to better understand the course and natural histo...

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Autores principales: Murall, Carmen Lía, Rahmoun, Massilva, Selinger, Christian, Baldellou, Monique, Bernat, Claire, Bonneau, Marine, Boué, Vanina, Buisson, Mathilde, Christophe, Guillaume, D’Auria, Giuseppe, Taroni, Florence De, Foulongne, Vincent, Froissart, Rémy, Graf, Christelle, Grasset, Sophie, Groc, Soraya, Hirtz, Christophe, Jaussent, Audrey, Lajoie, Julie, Lorcy, Frédérique, Picot, Eric, Picot, Marie-Christine, Ravel, Jacques, Reynes, Jacques, Rousset, Thérèse, Seddiki, Aziza, Teirlinck, Martine, Tribout, Vincent, Tuaillon, Édouard, Waterboer, Tim, Jacobs, Nathalie, Bravo, Ignacio G, Segondy, Michel, Boulle, Nathalie, Alizon, Samuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6576111/
https://www.ncbi.nlm.nih.gov/pubmed/31189673
http://dx.doi.org/10.1136/bmjopen-2018-025129
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author Murall, Carmen Lía
Rahmoun, Massilva
Selinger, Christian
Baldellou, Monique
Bernat, Claire
Bonneau, Marine
Boué, Vanina
Buisson, Mathilde
Christophe, Guillaume
D’Auria, Giuseppe
Taroni, Florence De
Foulongne, Vincent
Froissart, Rémy
Graf, Christelle
Grasset, Sophie
Groc, Soraya
Hirtz, Christophe
Jaussent, Audrey
Lajoie, Julie
Lorcy, Frédérique
Picot, Eric
Picot, Marie-Christine
Ravel, Jacques
Reynes, Jacques
Rousset, Thérèse
Seddiki, Aziza
Teirlinck, Martine
Tribout, Vincent
Tuaillon, Édouard
Waterboer, Tim
Jacobs, Nathalie
Bravo, Ignacio G
Segondy, Michel
Boulle, Nathalie
Alizon, Samuel
author_facet Murall, Carmen Lía
Rahmoun, Massilva
Selinger, Christian
Baldellou, Monique
Bernat, Claire
Bonneau, Marine
Boué, Vanina
Buisson, Mathilde
Christophe, Guillaume
D’Auria, Giuseppe
Taroni, Florence De
Foulongne, Vincent
Froissart, Rémy
Graf, Christelle
Grasset, Sophie
Groc, Soraya
Hirtz, Christophe
Jaussent, Audrey
Lajoie, Julie
Lorcy, Frédérique
Picot, Eric
Picot, Marie-Christine
Ravel, Jacques
Reynes, Jacques
Rousset, Thérèse
Seddiki, Aziza
Teirlinck, Martine
Tribout, Vincent
Tuaillon, Édouard
Waterboer, Tim
Jacobs, Nathalie
Bravo, Ignacio G
Segondy, Michel
Boulle, Nathalie
Alizon, Samuel
author_sort Murall, Carmen Lía
collection PubMed
description INTRODUCTION: Human papillomaviruses (HPVs) are responsible for one-third of all cancers caused by infections. Most HPV studies focus on chronic infections and cancers, and we know little about the early stages of the infection. Our main objective is to better understand the course and natural history of cervical HPV infections in healthy, unvaccinated and vaccinated, young women, by characterising the dynamics of various infection-related populations (virus, epithelial cells, vaginal microbiota and immune effectors). Another objective is to analyse HPV diversity within hosts, and in the study population, in relation to co-factors (lifestyle characteristics, vaccination status, vaginal microbiota, human genetics). METHODS AND ANALYSIS: The PAPCLEAR study is a single center longitudinal study following 150 women, aged 18–25 years, for up to 2 years. Visits occur every 2 or 4 months (depending on HPV status) during which several variables are measured, such as behaviours (via questionnaires), vaginal pH, HPV presence and viral load (via qPCR), local concentrations of cytokines (via MesoScale Discovery technology) and immune cells (via flow cytometry). Additional analyses are outsourced, such as titration of circulating anti-HPV antibodies, vaginal microbiota sequencing (16S and ITS1 loci) and human genotyping. To increase the statistical power of the epidemiological arm of the study, an additional 150 women are screened cross-sectionally. Finally, to maximise the resolution of the time series, participants are asked to perform weekly self-samples at home. Statistical analyses will involve classical tools in epidemiology, genomics and virus kinetics, and will be performed or coordinated by the Centre National de la Recherche Scientifique (CNRS) in Montpellier. ETHICS AND DISSEMINATION: This study has been approved by the Comité de Protection des Personnes Sud Méditerranée I (reference number 2016-A00712-49); by the Comité Consultatif sur le Traitement de l’Information en matière de Recherche dans le domaine de la Santé (reference number 16.504); by the Commission Nationale Informatique et Libertés (reference number MMS/ABD/AR1612278, decision number DR-2016–488) and by the Agence Nationale de Sécurité du Médicament et des Produits de Santé (reference 20160072000007). Results will be published in preprint servers, peer-reviewed journals and disseminated through conferences. TRIAL REGISTRATION NUMBER: NCT02946346; Pre-results.
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spelling pubmed-65761112019-07-02 Natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the PAPCLEAR cohort study Murall, Carmen Lía Rahmoun, Massilva Selinger, Christian Baldellou, Monique Bernat, Claire Bonneau, Marine Boué, Vanina Buisson, Mathilde Christophe, Guillaume D’Auria, Giuseppe Taroni, Florence De Foulongne, Vincent Froissart, Rémy Graf, Christelle Grasset, Sophie Groc, Soraya Hirtz, Christophe Jaussent, Audrey Lajoie, Julie Lorcy, Frédérique Picot, Eric Picot, Marie-Christine Ravel, Jacques Reynes, Jacques Rousset, Thérèse Seddiki, Aziza Teirlinck, Martine Tribout, Vincent Tuaillon, Édouard Waterboer, Tim Jacobs, Nathalie Bravo, Ignacio G Segondy, Michel Boulle, Nathalie Alizon, Samuel BMJ Open Infectious Diseases INTRODUCTION: Human papillomaviruses (HPVs) are responsible for one-third of all cancers caused by infections. Most HPV studies focus on chronic infections and cancers, and we know little about the early stages of the infection. Our main objective is to better understand the course and natural history of cervical HPV infections in healthy, unvaccinated and vaccinated, young women, by characterising the dynamics of various infection-related populations (virus, epithelial cells, vaginal microbiota and immune effectors). Another objective is to analyse HPV diversity within hosts, and in the study population, in relation to co-factors (lifestyle characteristics, vaccination status, vaginal microbiota, human genetics). METHODS AND ANALYSIS: The PAPCLEAR study is a single center longitudinal study following 150 women, aged 18–25 years, for up to 2 years. Visits occur every 2 or 4 months (depending on HPV status) during which several variables are measured, such as behaviours (via questionnaires), vaginal pH, HPV presence and viral load (via qPCR), local concentrations of cytokines (via MesoScale Discovery technology) and immune cells (via flow cytometry). Additional analyses are outsourced, such as titration of circulating anti-HPV antibodies, vaginal microbiota sequencing (16S and ITS1 loci) and human genotyping. To increase the statistical power of the epidemiological arm of the study, an additional 150 women are screened cross-sectionally. Finally, to maximise the resolution of the time series, participants are asked to perform weekly self-samples at home. Statistical analyses will involve classical tools in epidemiology, genomics and virus kinetics, and will be performed or coordinated by the Centre National de la Recherche Scientifique (CNRS) in Montpellier. ETHICS AND DISSEMINATION: This study has been approved by the Comité de Protection des Personnes Sud Méditerranée I (reference number 2016-A00712-49); by the Comité Consultatif sur le Traitement de l’Information en matière de Recherche dans le domaine de la Santé (reference number 16.504); by the Commission Nationale Informatique et Libertés (reference number MMS/ABD/AR1612278, decision number DR-2016–488) and by the Agence Nationale de Sécurité du Médicament et des Produits de Santé (reference 20160072000007). Results will be published in preprint servers, peer-reviewed journals and disseminated through conferences. TRIAL REGISTRATION NUMBER: NCT02946346; Pre-results. BMJ Publishing Group 2019-06-11 /pmc/articles/PMC6576111/ /pubmed/31189673 http://dx.doi.org/10.1136/bmjopen-2018-025129 Text en © Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Infectious Diseases
Murall, Carmen Lía
Rahmoun, Massilva
Selinger, Christian
Baldellou, Monique
Bernat, Claire
Bonneau, Marine
Boué, Vanina
Buisson, Mathilde
Christophe, Guillaume
D’Auria, Giuseppe
Taroni, Florence De
Foulongne, Vincent
Froissart, Rémy
Graf, Christelle
Grasset, Sophie
Groc, Soraya
Hirtz, Christophe
Jaussent, Audrey
Lajoie, Julie
Lorcy, Frédérique
Picot, Eric
Picot, Marie-Christine
Ravel, Jacques
Reynes, Jacques
Rousset, Thérèse
Seddiki, Aziza
Teirlinck, Martine
Tribout, Vincent
Tuaillon, Édouard
Waterboer, Tim
Jacobs, Nathalie
Bravo, Ignacio G
Segondy, Michel
Boulle, Nathalie
Alizon, Samuel
Natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the PAPCLEAR cohort study
title Natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the PAPCLEAR cohort study
title_full Natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the PAPCLEAR cohort study
title_fullStr Natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the PAPCLEAR cohort study
title_full_unstemmed Natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the PAPCLEAR cohort study
title_short Natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the PAPCLEAR cohort study
title_sort natural history, dynamics, and ecology of human papillomaviruses in genital infections of young women: protocol of the papclear cohort study
topic Infectious Diseases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6576111/
https://www.ncbi.nlm.nih.gov/pubmed/31189673
http://dx.doi.org/10.1136/bmjopen-2018-025129
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