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Conformational switches control early maturation of the eukaryotic small ribosomal subunit

Eukaryotic ribosome biogenesis is initiated with the transcription of pre-ribosomal RNA at the 5’ external transcribed spacer, which directs the early association of assembly factors but is absent from the mature ribosome. The subsequent co-transcriptional association of ribosome assembly factors wi...

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Autores principales: Hunziker, Mirjam, Barandun, Jonas, Buzovetsky, Olga, Steckler, Caitlin, Molina, Henrik, Klinge, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579516/
https://www.ncbi.nlm.nih.gov/pubmed/31206356
http://dx.doi.org/10.7554/eLife.45185
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author Hunziker, Mirjam
Barandun, Jonas
Buzovetsky, Olga
Steckler, Caitlin
Molina, Henrik
Klinge, Sebastian
author_facet Hunziker, Mirjam
Barandun, Jonas
Buzovetsky, Olga
Steckler, Caitlin
Molina, Henrik
Klinge, Sebastian
author_sort Hunziker, Mirjam
collection PubMed
description Eukaryotic ribosome biogenesis is initiated with the transcription of pre-ribosomal RNA at the 5’ external transcribed spacer, which directs the early association of assembly factors but is absent from the mature ribosome. The subsequent co-transcriptional association of ribosome assembly factors with pre-ribosomal RNA results in the formation of the small subunit processome. Here we show that stable rRNA domains of the small ribosomal subunit can independently recruit their own biogenesis factors in vivo. The final assembly and compaction of the small subunit processome requires the presence of the 5’ external transcribed spacer RNA and all ribosomal RNA domains. Additionally, our cryo-electron microscopy structure of the earliest nucleolar pre-ribosomal assembly - the 5’ external transcribed spacer ribonucleoprotein – provides a mechanism for how conformational changes in multi-protein complexes can be employed to regulate the accessibility of binding sites and therefore define the chronology of maturation events during early stages of ribosome assembly.
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spelling pubmed-65795162019-06-19 Conformational switches control early maturation of the eukaryotic small ribosomal subunit Hunziker, Mirjam Barandun, Jonas Buzovetsky, Olga Steckler, Caitlin Molina, Henrik Klinge, Sebastian eLife Biochemistry and Chemical Biology Eukaryotic ribosome biogenesis is initiated with the transcription of pre-ribosomal RNA at the 5’ external transcribed spacer, which directs the early association of assembly factors but is absent from the mature ribosome. The subsequent co-transcriptional association of ribosome assembly factors with pre-ribosomal RNA results in the formation of the small subunit processome. Here we show that stable rRNA domains of the small ribosomal subunit can independently recruit their own biogenesis factors in vivo. The final assembly and compaction of the small subunit processome requires the presence of the 5’ external transcribed spacer RNA and all ribosomal RNA domains. Additionally, our cryo-electron microscopy structure of the earliest nucleolar pre-ribosomal assembly - the 5’ external transcribed spacer ribonucleoprotein – provides a mechanism for how conformational changes in multi-protein complexes can be employed to regulate the accessibility of binding sites and therefore define the chronology of maturation events during early stages of ribosome assembly. eLife Sciences Publications, Ltd 2019-06-17 /pmc/articles/PMC6579516/ /pubmed/31206356 http://dx.doi.org/10.7554/eLife.45185 Text en © 2019, Hunziker et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Hunziker, Mirjam
Barandun, Jonas
Buzovetsky, Olga
Steckler, Caitlin
Molina, Henrik
Klinge, Sebastian
Conformational switches control early maturation of the eukaryotic small ribosomal subunit
title Conformational switches control early maturation of the eukaryotic small ribosomal subunit
title_full Conformational switches control early maturation of the eukaryotic small ribosomal subunit
title_fullStr Conformational switches control early maturation of the eukaryotic small ribosomal subunit
title_full_unstemmed Conformational switches control early maturation of the eukaryotic small ribosomal subunit
title_short Conformational switches control early maturation of the eukaryotic small ribosomal subunit
title_sort conformational switches control early maturation of the eukaryotic small ribosomal subunit
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579516/
https://www.ncbi.nlm.nih.gov/pubmed/31206356
http://dx.doi.org/10.7554/eLife.45185
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