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Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy
Bacteriophages can be used successfully to treat pathogenic bacteria in the food chain including zoonotic pathogens that colonize the intestines of farm animals. However, harsh gastric conditions of low pH and digestive enzyme activities affect phage viability, and accordingly reduce their effective...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579803/ https://www.ncbi.nlm.nih.gov/pubmed/31209685 http://dx.doi.org/10.1186/s13568-019-0810-9 |
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author | Abdelsattar, Abdallah S. Abdelrahman, Fatma Dawoud, Alyaa Connerton, Ian F. El-Shibiny, Ayman |
author_facet | Abdelsattar, Abdallah S. Abdelrahman, Fatma Dawoud, Alyaa Connerton, Ian F. El-Shibiny, Ayman |
author_sort | Abdelsattar, Abdallah S. |
collection | PubMed |
description | Bacteriophages can be used successfully to treat pathogenic bacteria in the food chain including zoonotic pathogens that colonize the intestines of farm animals. However, harsh gastric conditions of low pH and digestive enzyme activities affect phage viability, and accordingly reduce their effectiveness. We report the development of a natural protective barrier suitable for oral administration to farm animals that confers acid stability before functional release of bead-encapsulated phages. Escherichia coli bacteriophage ZSEC5 is rendered inactive at pH 2.0 but encapsulation in chitosan–alginate bead with a honey and gelatin matrix limited titer reductions to 1 log(10) PFU mL(−1). The encapsulated phage titers were stable upon storage in water but achieved near complete release over 4–5 h in a simulated intestinal solution (0.1% bile salt, 0.4% pancreatin, 50 mM KH(2)PO(4) pH 7.5) at 37 °C. Exposure of E. coli O157:H7 to the bead-encapsulated phage preparations produced a delayed response, reaching a maximal reductions of 4.2 to 4.8 log(10) CFU mL(−1) after 10 h at 37 °C under simulated intestinal conditions compared to a maximal reduction of 5.1 log(10) CFU mL(−1) at 3 h for free phage applied at MOI = 1. Bead-encapsulation is a promising reliable and cost-effective method for the functional delivery of bacteriophage targeting intestinal bacteria of farm animals. |
format | Online Article Text |
id | pubmed-6579803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-65798032019-07-05 Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy Abdelsattar, Abdallah S. Abdelrahman, Fatma Dawoud, Alyaa Connerton, Ian F. El-Shibiny, Ayman AMB Express Original Article Bacteriophages can be used successfully to treat pathogenic bacteria in the food chain including zoonotic pathogens that colonize the intestines of farm animals. However, harsh gastric conditions of low pH and digestive enzyme activities affect phage viability, and accordingly reduce their effectiveness. We report the development of a natural protective barrier suitable for oral administration to farm animals that confers acid stability before functional release of bead-encapsulated phages. Escherichia coli bacteriophage ZSEC5 is rendered inactive at pH 2.0 but encapsulation in chitosan–alginate bead with a honey and gelatin matrix limited titer reductions to 1 log(10) PFU mL(−1). The encapsulated phage titers were stable upon storage in water but achieved near complete release over 4–5 h in a simulated intestinal solution (0.1% bile salt, 0.4% pancreatin, 50 mM KH(2)PO(4) pH 7.5) at 37 °C. Exposure of E. coli O157:H7 to the bead-encapsulated phage preparations produced a delayed response, reaching a maximal reductions of 4.2 to 4.8 log(10) CFU mL(−1) after 10 h at 37 °C under simulated intestinal conditions compared to a maximal reduction of 5.1 log(10) CFU mL(−1) at 3 h for free phage applied at MOI = 1. Bead-encapsulation is a promising reliable and cost-effective method for the functional delivery of bacteriophage targeting intestinal bacteria of farm animals. Springer Berlin Heidelberg 2019-06-17 /pmc/articles/PMC6579803/ /pubmed/31209685 http://dx.doi.org/10.1186/s13568-019-0810-9 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Abdelsattar, Abdallah S. Abdelrahman, Fatma Dawoud, Alyaa Connerton, Ian F. El-Shibiny, Ayman Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy |
title | Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy |
title_full | Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy |
title_fullStr | Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy |
title_full_unstemmed | Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy |
title_short | Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy |
title_sort | encapsulation of e. coli phage zcec5 in chitosan–alginate beads as a delivery system in phage therapy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579803/ https://www.ncbi.nlm.nih.gov/pubmed/31209685 http://dx.doi.org/10.1186/s13568-019-0810-9 |
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