Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics

High-throughput single-cell technologies have recently emerged as essential tools in biomedical research with great potential for clinical pathology when studying liquid and solid biopsies. We provide an update on current single-cell methods in cerebrospinal fluid research and diagnostics, focusing...

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Autores principales: Lanz, Tobias V., Pröbstel, Anne-Katrin, Mildenberger, Iris, Platten, Michael, Schirmer, Lucas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579921/
https://www.ncbi.nlm.nih.gov/pubmed/31244848
http://dx.doi.org/10.3389/fimmu.2019.01302
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author Lanz, Tobias V.
Pröbstel, Anne-Katrin
Mildenberger, Iris
Platten, Michael
Schirmer, Lucas
author_facet Lanz, Tobias V.
Pröbstel, Anne-Katrin
Mildenberger, Iris
Platten, Michael
Schirmer, Lucas
author_sort Lanz, Tobias V.
collection PubMed
description High-throughput single-cell technologies have recently emerged as essential tools in biomedical research with great potential for clinical pathology when studying liquid and solid biopsies. We provide an update on current single-cell methods in cerebrospinal fluid research and diagnostics, focusing on high-throughput cell-type specific proteomic and genomic technologies. Proteomic methods comprising flow cytometry and mass cytometry as well as genomic approaches including immune cell repertoire and single-cell transcriptomic studies are critically reviewed and future directions discussed.
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spelling pubmed-65799212019-06-26 Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics Lanz, Tobias V. Pröbstel, Anne-Katrin Mildenberger, Iris Platten, Michael Schirmer, Lucas Front Immunol Immunology High-throughput single-cell technologies have recently emerged as essential tools in biomedical research with great potential for clinical pathology when studying liquid and solid biopsies. We provide an update on current single-cell methods in cerebrospinal fluid research and diagnostics, focusing on high-throughput cell-type specific proteomic and genomic technologies. Proteomic methods comprising flow cytometry and mass cytometry as well as genomic approaches including immune cell repertoire and single-cell transcriptomic studies are critically reviewed and future directions discussed. Frontiers Media S.A. 2019-06-11 /pmc/articles/PMC6579921/ /pubmed/31244848 http://dx.doi.org/10.3389/fimmu.2019.01302 Text en Copyright © 2019 Lanz, Pröbstel, Mildenberger, Platten and Schirmer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Lanz, Tobias V.
Pröbstel, Anne-Katrin
Mildenberger, Iris
Platten, Michael
Schirmer, Lucas
Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics
title Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics
title_full Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics
title_fullStr Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics
title_full_unstemmed Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics
title_short Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics
title_sort single-cell high-throughput technologies in cerebrospinal fluid research and diagnostics
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579921/
https://www.ncbi.nlm.nih.gov/pubmed/31244848
http://dx.doi.org/10.3389/fimmu.2019.01302
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