Cargando…

Identification of claudin-1, −3, −7 and −8 as prognostic markers in human laryngeal carcinoma

Various genomic and epigenetic modifications that occur during the development of cancer act as potential biomarkers for early diagnosis and treatment. Previous studies have demonstrated abnormal expression of the claudin (CLDN) tight junction (TJ) proteins in numerous types of human cancer. Reverse...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Shu, Piao, Xue, Wang, Chengyan, Wang, Rui, Song, Zhimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580001/
https://www.ncbi.nlm.nih.gov/pubmed/31115553
http://dx.doi.org/10.3892/mmr.2019.10265
_version_ 1783427948538232832
author Zhou, Shu
Piao, Xue
Wang, Chengyan
Wang, Rui
Song, Zhimin
author_facet Zhou, Shu
Piao, Xue
Wang, Chengyan
Wang, Rui
Song, Zhimin
author_sort Zhou, Shu
collection PubMed
description Various genomic and epigenetic modifications that occur during the development of cancer act as potential biomarkers for early diagnosis and treatment. Previous studies have demonstrated abnormal expression of the claudin (CLDN) tight junction (TJ) proteins in numerous types of human cancer. Reverse transcription-quantitative polymerase chain reaction and western blotting were employed to investigate variations in the expression of the CLDN TJ proteins in laryngeal non-neoplastic tissues and laryngeal squamous carcinoma tissues. It was revealed that CLDN2, CLDN4, CLDN5, CLDN6, CLDN9, CLDN11 and CLDN12 were undetectable in laryngeal squamous carcinoma tissues and laryngeal non-neoplastic tissues. Additionally, CLDN10 was expressed in laryngeal squamous carcinoma tissues and laryngeal non-neoplastic tissues; however, no significant difference was reported. Conversely, the expression levels of CLDN1 and CLDN7 mRNA and protein were downregulated in laryngeal squamous carcinoma tissues compared with in adjacent non-neoplastic tissues, whereas those of CLDN3 and CLDN8 were upregulated. A total of 80 samples of laryngeal squamous carcinoma and non-neoplastic tissues were analyzed for the expression of CLDN1, −3, −7 and −8 via streptavidin-peroxidase immunohistochemical staining. It was revealed that the expression levels of CLDN1 and CLDN7 were downregulated in laryngeal squamous carcinoma tissues compared with in non-neoplastic mucosal tissues, whereas those of CLDN3 and CLDN8 were upregulated. Furthermore, the associations between CLDN expression and the clinicopathological factors of patients were analyzed. The expression levels of CLDN3 and CLDN7 were reported to be associated with distant metastasis and serve as potential predictors of poor prognosis. In conclusion, the findings of the present study demonstrated that the expression levels of CLDN1, −3, −7 and −8 varied between laryngeal squamous carcinoma tissues and non-neoplastic tissues. The expression levels of these CLDNs may be useful molecular markers for the diagnosis of laryngeal carcinoma, and determining the metastasis and prognosis of this disease.
format Online
Article
Text
id pubmed-6580001
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-65800012019-07-05 Identification of claudin-1, −3, −7 and −8 as prognostic markers in human laryngeal carcinoma Zhou, Shu Piao, Xue Wang, Chengyan Wang, Rui Song, Zhimin Mol Med Rep Articles Various genomic and epigenetic modifications that occur during the development of cancer act as potential biomarkers for early diagnosis and treatment. Previous studies have demonstrated abnormal expression of the claudin (CLDN) tight junction (TJ) proteins in numerous types of human cancer. Reverse transcription-quantitative polymerase chain reaction and western blotting were employed to investigate variations in the expression of the CLDN TJ proteins in laryngeal non-neoplastic tissues and laryngeal squamous carcinoma tissues. It was revealed that CLDN2, CLDN4, CLDN5, CLDN6, CLDN9, CLDN11 and CLDN12 were undetectable in laryngeal squamous carcinoma tissues and laryngeal non-neoplastic tissues. Additionally, CLDN10 was expressed in laryngeal squamous carcinoma tissues and laryngeal non-neoplastic tissues; however, no significant difference was reported. Conversely, the expression levels of CLDN1 and CLDN7 mRNA and protein were downregulated in laryngeal squamous carcinoma tissues compared with in adjacent non-neoplastic tissues, whereas those of CLDN3 and CLDN8 were upregulated. A total of 80 samples of laryngeal squamous carcinoma and non-neoplastic tissues were analyzed for the expression of CLDN1, −3, −7 and −8 via streptavidin-peroxidase immunohistochemical staining. It was revealed that the expression levels of CLDN1 and CLDN7 were downregulated in laryngeal squamous carcinoma tissues compared with in non-neoplastic mucosal tissues, whereas those of CLDN3 and CLDN8 were upregulated. Furthermore, the associations between CLDN expression and the clinicopathological factors of patients were analyzed. The expression levels of CLDN3 and CLDN7 were reported to be associated with distant metastasis and serve as potential predictors of poor prognosis. In conclusion, the findings of the present study demonstrated that the expression levels of CLDN1, −3, −7 and −8 varied between laryngeal squamous carcinoma tissues and non-neoplastic tissues. The expression levels of these CLDNs may be useful molecular markers for the diagnosis of laryngeal carcinoma, and determining the metastasis and prognosis of this disease. D.A. Spandidos 2019-07 2019-05-22 /pmc/articles/PMC6580001/ /pubmed/31115553 http://dx.doi.org/10.3892/mmr.2019.10265 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhou, Shu
Piao, Xue
Wang, Chengyan
Wang, Rui
Song, Zhimin
Identification of claudin-1, −3, −7 and −8 as prognostic markers in human laryngeal carcinoma
title Identification of claudin-1, −3, −7 and −8 as prognostic markers in human laryngeal carcinoma
title_full Identification of claudin-1, −3, −7 and −8 as prognostic markers in human laryngeal carcinoma
title_fullStr Identification of claudin-1, −3, −7 and −8 as prognostic markers in human laryngeal carcinoma
title_full_unstemmed Identification of claudin-1, −3, −7 and −8 as prognostic markers in human laryngeal carcinoma
title_short Identification of claudin-1, −3, −7 and −8 as prognostic markers in human laryngeal carcinoma
title_sort identification of claudin-1, −3, −7 and −8 as prognostic markers in human laryngeal carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580001/
https://www.ncbi.nlm.nih.gov/pubmed/31115553
http://dx.doi.org/10.3892/mmr.2019.10265
work_keys_str_mv AT zhoushu identificationofclaudin137and8asprognosticmarkersinhumanlaryngealcarcinoma
AT piaoxue identificationofclaudin137and8asprognosticmarkersinhumanlaryngealcarcinoma
AT wangchengyan identificationofclaudin137and8asprognosticmarkersinhumanlaryngealcarcinoma
AT wangrui identificationofclaudin137and8asprognosticmarkersinhumanlaryngealcarcinoma
AT songzhimin identificationofclaudin137and8asprognosticmarkersinhumanlaryngealcarcinoma