CM01: a facility for cryo-electron microscopy at the European Synchrotron

Recent improvements in direct electron detectors, microscope technology and software provided the stimulus for a ‘quantum leap’ in the application of cryo-electron microscopy in structural biology, and many national and international centres have since been created in order to exploit this. Here, a...

Descripción completa

Detalles Bibliográficos
Autores principales: Kandiah, Eaazhisai, Giraud, Thierry, de Maria Antolinos, Alejandro, Dobias, Fabien, Effantin, Gregory, Flot, David, Hons, Michael, Schoehn, Guy, Susini, Jean, Svensson, Olof, Leonard, Gordon A., Mueller-Dieckmann, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2019
Materias:
Feature Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580229/
https://www.ncbi.nlm.nih.gov/pubmed/31205015
http://dx.doi.org/10.1107/S2059798319006880
Descripción
Sumario:Recent improvements in direct electron detectors, microscope technology and software provided the stimulus for a ‘quantum leap’ in the application of cryo-electron microscopy in structural biology, and many national and international centres have since been created in order to exploit this. Here, a new facility for cryo-electron microscopy focused on single-particle reconstruction of biological macromolecules that has been commissioned at the European Synchrotron Radiation Facility (ESRF) is presented. The facility is operated by a consortium of institutes co-located on the European Photon and Neutron Campus and is managed in a similar fashion to a synchrotron X-ray beamline. It has been open to the ESRF structural biology user community since November 2017 and will remain open during the 2019 ESRF–EBS shutdown.

Ejemplares similares