Cargando…

A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative tolerance in rice

BACKGROUND: Drought is a major abiotic stress factor that influences the yield of crops. Basic leucine zipper motif (bZIP) transcription factors play an important regulatory role in plant drought stress responses. However, the functions of a number of bZIP transcription factors in rice are still unk...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Shiqin, Xu, Kai, Chen, Shoujun, Li, Tianfei, Xia, Hui, Chen, Liang, Liu, Hongyan, Luo, Lijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580479/
https://www.ncbi.nlm.nih.gov/pubmed/31208338
http://dx.doi.org/10.1186/s12870-019-1872-1
_version_ 1783428027607154688
author Yang, Shiqin
Xu, Kai
Chen, Shoujun
Li, Tianfei
Xia, Hui
Chen, Liang
Liu, Hongyan
Luo, Lijun
author_facet Yang, Shiqin
Xu, Kai
Chen, Shoujun
Li, Tianfei
Xia, Hui
Chen, Liang
Liu, Hongyan
Luo, Lijun
author_sort Yang, Shiqin
collection PubMed
description BACKGROUND: Drought is a major abiotic stress factor that influences the yield of crops. Basic leucine zipper motif (bZIP) transcription factors play an important regulatory role in plant drought stress responses. However, the functions of a number of bZIP transcription factors in rice are still unknown. RESULTS: In this study, a novel drought stress-related bZIP transcription factor, OsbZIP62, was identified in rice. This gene was selected from a transcriptome analysis of several typical rice varieties with different drought tolerances. OsbZIP62 expression was induced by drought, hydrogen peroxide, and abscisic acid (ABA) treatment. Overexpression of OsbZIP62-VP64 (OsbZIP62V) enhanced the drought tolerance and oxidative stress tolerance of transgenic rice, while osbzip62 mutants exhibited the opposite phenotype. OsbZIP62-GFP was localized to the nucleus, and the N-terminal sequence (amino acids 1–68) was necessary for the transcriptional activation activity of OsbZIP62. RNA-seq analysis showed that the expression of many stress-related genes (e.g., OsGL1, OsNAC10, and DSM2) was upregulated in OsbZIP62V plants. Moreover, OsbZIP62 could bind to the promoters of several putative target genes and could interact with stress/ABA-activated protein kinases (SAPKs). CONCLUSIONS: OsbZIP62 is involved in ABA signalling pathways and positively regulates rice drought tolerance by regulating the expression of genes associated with stress, and this gene could be used for the genetic modification of crops with improved drought tolerance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1872-1) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-6580479
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-65804792019-06-24 A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative tolerance in rice Yang, Shiqin Xu, Kai Chen, Shoujun Li, Tianfei Xia, Hui Chen, Liang Liu, Hongyan Luo, Lijun BMC Plant Biol Research Article BACKGROUND: Drought is a major abiotic stress factor that influences the yield of crops. Basic leucine zipper motif (bZIP) transcription factors play an important regulatory role in plant drought stress responses. However, the functions of a number of bZIP transcription factors in rice are still unknown. RESULTS: In this study, a novel drought stress-related bZIP transcription factor, OsbZIP62, was identified in rice. This gene was selected from a transcriptome analysis of several typical rice varieties with different drought tolerances. OsbZIP62 expression was induced by drought, hydrogen peroxide, and abscisic acid (ABA) treatment. Overexpression of OsbZIP62-VP64 (OsbZIP62V) enhanced the drought tolerance and oxidative stress tolerance of transgenic rice, while osbzip62 mutants exhibited the opposite phenotype. OsbZIP62-GFP was localized to the nucleus, and the N-terminal sequence (amino acids 1–68) was necessary for the transcriptional activation activity of OsbZIP62. RNA-seq analysis showed that the expression of many stress-related genes (e.g., OsGL1, OsNAC10, and DSM2) was upregulated in OsbZIP62V plants. Moreover, OsbZIP62 could bind to the promoters of several putative target genes and could interact with stress/ABA-activated protein kinases (SAPKs). CONCLUSIONS: OsbZIP62 is involved in ABA signalling pathways and positively regulates rice drought tolerance by regulating the expression of genes associated with stress, and this gene could be used for the genetic modification of crops with improved drought tolerance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1872-1) contains supplementary material, which is available to authorized users. BioMed Central 2019-06-17 /pmc/articles/PMC6580479/ /pubmed/31208338 http://dx.doi.org/10.1186/s12870-019-1872-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yang, Shiqin
Xu, Kai
Chen, Shoujun
Li, Tianfei
Xia, Hui
Chen, Liang
Liu, Hongyan
Luo, Lijun
A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative tolerance in rice
title A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative tolerance in rice
title_full A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative tolerance in rice
title_fullStr A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative tolerance in rice
title_full_unstemmed A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative tolerance in rice
title_short A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative tolerance in rice
title_sort stress-responsive bzip transcription factor osbzip62 improves drought and oxidative tolerance in rice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580479/
https://www.ncbi.nlm.nih.gov/pubmed/31208338
http://dx.doi.org/10.1186/s12870-019-1872-1
work_keys_str_mv AT yangshiqin astressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT xukai astressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT chenshoujun astressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT litianfei astressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT xiahui astressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT chenliang astressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT liuhongyan astressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT luolijun astressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT yangshiqin stressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT xukai stressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT chenshoujun stressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT litianfei stressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT xiahui stressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT chenliang stressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT liuhongyan stressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice
AT luolijun stressresponsivebziptranscriptionfactorosbzip62improvesdroughtandoxidativetoleranceinrice