Cargando…

Microsatellite markers for the biogeographically enigmatic sandmyrtle (Kalmia buxifolia, Phyllodoceae: Ericaceae)

PREMISE: Microsatellite markers were developed for sandmyrtle, Kalmia buxifolia (Ericaceae), to facilitate phylogeographic studies in this taxon and possibly many of its close relatives. METHODS AND RESULTS: Forty‐eight primer pairs designed from paired‐end Illumina MiSeq data were screened for robu...

Descripción completa

Detalles Bibliográficos
Autores principales: Gillespie, Emily L., Madsen‐McQueen, Tesa, Eriksson, Torsten, Bailey, Adam, Murrell, Zack E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580982/
https://www.ncbi.nlm.nih.gov/pubmed/31236314
http://dx.doi.org/10.1002/aps3.11267
_version_ 1783428112417030144
author Gillespie, Emily L.
Madsen‐McQueen, Tesa
Eriksson, Torsten
Bailey, Adam
Murrell, Zack E.
author_facet Gillespie, Emily L.
Madsen‐McQueen, Tesa
Eriksson, Torsten
Bailey, Adam
Murrell, Zack E.
author_sort Gillespie, Emily L.
collection PubMed
description PREMISE: Microsatellite markers were developed for sandmyrtle, Kalmia buxifolia (Ericaceae), to facilitate phylogeographic studies in this taxon and possibly many of its close relatives. METHODS AND RESULTS: Forty‐eight primer pairs designed from paired‐end Illumina MiSeq data were screened for robust amplification. Sixteen pairs were amplified again, but with fluorescently labeled primers to facilitate genotyping. Resulting chromatograms were evaluated for variability using three populations from Tennessee, North Carolina, and New Jersey, USA. Eleven primer pairs were reliable and polymorphic (mean 3.92 alleles), one was reliable but monomorphic, and four were not reliable. The markers exhibited lower heterozygosity (mean 0.246) than expected (mean 0.464). Cross‐amplification in the remaining nine Kalmia species exhibited a phylogenetic pattern, suggesting broad applicability of the markers across the genus. CONCLUSIONS: These microsatellite markers will be useful in population genetics and species boundaries studies of K. buxifolia, K. procumbens, and likely all other Kalmia species.
format Online
Article
Text
id pubmed-6580982
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-65809822019-06-24 Microsatellite markers for the biogeographically enigmatic sandmyrtle (Kalmia buxifolia, Phyllodoceae: Ericaceae) Gillespie, Emily L. Madsen‐McQueen, Tesa Eriksson, Torsten Bailey, Adam Murrell, Zack E. Appl Plant Sci Primer Notes PREMISE: Microsatellite markers were developed for sandmyrtle, Kalmia buxifolia (Ericaceae), to facilitate phylogeographic studies in this taxon and possibly many of its close relatives. METHODS AND RESULTS: Forty‐eight primer pairs designed from paired‐end Illumina MiSeq data were screened for robust amplification. Sixteen pairs were amplified again, but with fluorescently labeled primers to facilitate genotyping. Resulting chromatograms were evaluated for variability using three populations from Tennessee, North Carolina, and New Jersey, USA. Eleven primer pairs were reliable and polymorphic (mean 3.92 alleles), one was reliable but monomorphic, and four were not reliable. The markers exhibited lower heterozygosity (mean 0.246) than expected (mean 0.464). Cross‐amplification in the remaining nine Kalmia species exhibited a phylogenetic pattern, suggesting broad applicability of the markers across the genus. CONCLUSIONS: These microsatellite markers will be useful in population genetics and species boundaries studies of K. buxifolia, K. procumbens, and likely all other Kalmia species. John Wiley and Sons Inc. 2019-06-05 /pmc/articles/PMC6580982/ /pubmed/31236314 http://dx.doi.org/10.1002/aps3.11267 Text en © 2019 Gillespie et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primer Notes
Gillespie, Emily L.
Madsen‐McQueen, Tesa
Eriksson, Torsten
Bailey, Adam
Murrell, Zack E.
Microsatellite markers for the biogeographically enigmatic sandmyrtle (Kalmia buxifolia, Phyllodoceae: Ericaceae)
title Microsatellite markers for the biogeographically enigmatic sandmyrtle (Kalmia buxifolia, Phyllodoceae: Ericaceae)
title_full Microsatellite markers for the biogeographically enigmatic sandmyrtle (Kalmia buxifolia, Phyllodoceae: Ericaceae)
title_fullStr Microsatellite markers for the biogeographically enigmatic sandmyrtle (Kalmia buxifolia, Phyllodoceae: Ericaceae)
title_full_unstemmed Microsatellite markers for the biogeographically enigmatic sandmyrtle (Kalmia buxifolia, Phyllodoceae: Ericaceae)
title_short Microsatellite markers for the biogeographically enigmatic sandmyrtle (Kalmia buxifolia, Phyllodoceae: Ericaceae)
title_sort microsatellite markers for the biogeographically enigmatic sandmyrtle (kalmia buxifolia, phyllodoceae: ericaceae)
topic Primer Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580982/
https://www.ncbi.nlm.nih.gov/pubmed/31236314
http://dx.doi.org/10.1002/aps3.11267
work_keys_str_mv AT gillespieemilyl microsatellitemarkersforthebiogeographicallyenigmaticsandmyrtlekalmiabuxifoliaphyllodoceaeericaceae
AT madsenmcqueentesa microsatellitemarkersforthebiogeographicallyenigmaticsandmyrtlekalmiabuxifoliaphyllodoceaeericaceae
AT erikssontorsten microsatellitemarkersforthebiogeographicallyenigmaticsandmyrtlekalmiabuxifoliaphyllodoceaeericaceae
AT baileyadam microsatellitemarkersforthebiogeographicallyenigmaticsandmyrtlekalmiabuxifoliaphyllodoceaeericaceae
AT murrellzacke microsatellitemarkersforthebiogeographicallyenigmaticsandmyrtlekalmiabuxifoliaphyllodoceaeericaceae