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New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae)

PREMISE: Algarrobilla (Balsamocarpon brevifolium, Fabaceae) is an endemic xerophytic shrub restricted to the Atacama Desert in northern Chile. Extensive utilization of the region for coal production has endangered this species. Conservation efforts are underway, with genetic diversity analyses being...

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Autores principales: Ravest, Gonzalo, León‐Lobos, Pedro, Aguirre, Carlos, Hernández, José, Bolados, Gustavo, Castro, María Herminia, Silva, Sergio, Hinrichsen, Patricio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580986/
https://www.ncbi.nlm.nih.gov/pubmed/31236318
http://dx.doi.org/10.1002/aps3.11271
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author Ravest, Gonzalo
León‐Lobos, Pedro
Aguirre, Carlos
Hernández, José
Bolados, Gustavo
Castro, María Herminia
Silva, Sergio
Hinrichsen, Patricio
author_facet Ravest, Gonzalo
León‐Lobos, Pedro
Aguirre, Carlos
Hernández, José
Bolados, Gustavo
Castro, María Herminia
Silva, Sergio
Hinrichsen, Patricio
author_sort Ravest, Gonzalo
collection PubMed
description PREMISE: Algarrobilla (Balsamocarpon brevifolium, Fabaceae) is an endemic xerophytic shrub restricted to the Atacama Desert in northern Chile. Extensive utilization of the region for coal production has endangered this species. Conservation efforts are underway, with genetic diversity analyses being key to the restoration of these populations. METHODS AND RESULTS: Fifteen new microsatellite markers were developed for B. brevifolium and used to analyze three populations from the Atacama and Coquimbo regions in Chile. Microsatellites were highly polymorphic, with an average of 5.77 alleles per marker and an average level of expected heterozygosity of 0.72. These markers were evaluated and cross‐amplified on two related species (Senna cumingii and Caesalpinia angulata) with partial success. CONCLUSIONS: The development of this set of markers permits an extensive study of B. brevifolium populations for conservation purposes.
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spelling pubmed-65809862019-06-24 New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae) Ravest, Gonzalo León‐Lobos, Pedro Aguirre, Carlos Hernández, José Bolados, Gustavo Castro, María Herminia Silva, Sergio Hinrichsen, Patricio Appl Plant Sci Primer Notes PREMISE: Algarrobilla (Balsamocarpon brevifolium, Fabaceae) is an endemic xerophytic shrub restricted to the Atacama Desert in northern Chile. Extensive utilization of the region for coal production has endangered this species. Conservation efforts are underway, with genetic diversity analyses being key to the restoration of these populations. METHODS AND RESULTS: Fifteen new microsatellite markers were developed for B. brevifolium and used to analyze three populations from the Atacama and Coquimbo regions in Chile. Microsatellites were highly polymorphic, with an average of 5.77 alleles per marker and an average level of expected heterozygosity of 0.72. These markers were evaluated and cross‐amplified on two related species (Senna cumingii and Caesalpinia angulata) with partial success. CONCLUSIONS: The development of this set of markers permits an extensive study of B. brevifolium populations for conservation purposes. John Wiley and Sons Inc. 2019-06-11 /pmc/articles/PMC6580986/ /pubmed/31236318 http://dx.doi.org/10.1002/aps3.11271 Text en © 2019 Ravest et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primer Notes
Ravest, Gonzalo
León‐Lobos, Pedro
Aguirre, Carlos
Hernández, José
Bolados, Gustavo
Castro, María Herminia
Silva, Sergio
Hinrichsen, Patricio
New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae)
title New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae)
title_full New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae)
title_fullStr New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae)
title_full_unstemmed New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae)
title_short New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae)
title_sort new microsatellites for the atacama desert endemic balsamocarpon brevifolium (fabaceae)
topic Primer Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580986/
https://www.ncbi.nlm.nih.gov/pubmed/31236318
http://dx.doi.org/10.1002/aps3.11271
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