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Association Between Hematological Parameters and Iron Metabolism Response After Marathon Race and ACTN3 Genotype

α-Actinin-3 (ACTN3 R577X, rs.1815739) polymorphism is a genetic variation that shows the most consistent influence on metabolic pathway and muscle phenotype. XX genotype is associated with higher metabolic efficiency of skeletal muscle; however, the role of ACTN3 polymorphism in oxygen transport and...

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Autores principales: Sierra, Ana Paula Renno, Oliveira, Rodrigo Assunção, Silva, Elton Dias, Lima, Giscard Humberto Oliveira, Benetti, Marino Pereira, Kiss, Maria Augusta Pedanti, Sierra, Carlos Anibal, Ghorayeb, Nabil, Seto, Jane T., Pesquero, João Bosco, Cury-Boaventura, Maria Fernanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580990/
https://www.ncbi.nlm.nih.gov/pubmed/31244673
http://dx.doi.org/10.3389/fphys.2019.00697
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author Sierra, Ana Paula Renno
Oliveira, Rodrigo Assunção
Silva, Elton Dias
Lima, Giscard Humberto Oliveira
Benetti, Marino Pereira
Kiss, Maria Augusta Pedanti
Sierra, Carlos Anibal
Ghorayeb, Nabil
Seto, Jane T.
Pesquero, João Bosco
Cury-Boaventura, Maria Fernanda
author_facet Sierra, Ana Paula Renno
Oliveira, Rodrigo Assunção
Silva, Elton Dias
Lima, Giscard Humberto Oliveira
Benetti, Marino Pereira
Kiss, Maria Augusta Pedanti
Sierra, Carlos Anibal
Ghorayeb, Nabil
Seto, Jane T.
Pesquero, João Bosco
Cury-Boaventura, Maria Fernanda
author_sort Sierra, Ana Paula Renno
collection PubMed
description α-Actinin-3 (ACTN3 R577X, rs.1815739) polymorphism is a genetic variation that shows the most consistent influence on metabolic pathway and muscle phenotype. XX genotype is associated with higher metabolic efficiency of skeletal muscle; however, the role of ACTN3 polymorphism in oxygen transport and utilization system has not yet been investigated. Therefore, the aim of this study was to determine the influence of ACTN3 polymorphisms on hematological and iron metabolism response induced by marathon race. Eighty-one Brazilian amateur male endurance runners participated in the study. Blood samples and urine were collected before; immediately after; and 1, 3, and 15 days after the marathon race. Urine, hematological parameters, iron metabolism, and ACTN3 genotyping analyses were performed. The marathon race induced a decrease in erythrocytes, Hb, and Ht, and an increase in hematuria, creatinine, myoglobin, red cell distribution width, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, direct and indirect bilirubin and erythropoietin. Moreover, an elevation immediately or 1 day after the marathon race follows a reduction 3 or 15 days after the marathon race were observed on transferrin saturation and iron and transferrin levels. Hematological parameters and iron metabolism changes induced by marathon race were not observed in XX genotypes. Hematuria and decreased erythrocytes, Hb, Ht, and iron and transferrin levels were observed only in RR and/or RX genotypes but not in XX genotypes. The percentage of runners with hematuria, leukocyturia, iron deficiency, creatinine, myoglobin, and bilirubin imbalance was higher in RR compared to XX genotypes. ACTN3 polymorphism is associated with iron metabolism and hematological responses after endurance exercise. Despite these results being based on a small sample, they highlight a protective role of the XX genotype on hematological and renal changes induced by long-distance exercise. Therefore, these findings should be further replicated.
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spelling pubmed-65809902019-06-26 Association Between Hematological Parameters and Iron Metabolism Response After Marathon Race and ACTN3 Genotype Sierra, Ana Paula Renno Oliveira, Rodrigo Assunção Silva, Elton Dias Lima, Giscard Humberto Oliveira Benetti, Marino Pereira Kiss, Maria Augusta Pedanti Sierra, Carlos Anibal Ghorayeb, Nabil Seto, Jane T. Pesquero, João Bosco Cury-Boaventura, Maria Fernanda Front Physiol Physiology α-Actinin-3 (ACTN3 R577X, rs.1815739) polymorphism is a genetic variation that shows the most consistent influence on metabolic pathway and muscle phenotype. XX genotype is associated with higher metabolic efficiency of skeletal muscle; however, the role of ACTN3 polymorphism in oxygen transport and utilization system has not yet been investigated. Therefore, the aim of this study was to determine the influence of ACTN3 polymorphisms on hematological and iron metabolism response induced by marathon race. Eighty-one Brazilian amateur male endurance runners participated in the study. Blood samples and urine were collected before; immediately after; and 1, 3, and 15 days after the marathon race. Urine, hematological parameters, iron metabolism, and ACTN3 genotyping analyses were performed. The marathon race induced a decrease in erythrocytes, Hb, and Ht, and an increase in hematuria, creatinine, myoglobin, red cell distribution width, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, direct and indirect bilirubin and erythropoietin. Moreover, an elevation immediately or 1 day after the marathon race follows a reduction 3 or 15 days after the marathon race were observed on transferrin saturation and iron and transferrin levels. Hematological parameters and iron metabolism changes induced by marathon race were not observed in XX genotypes. Hematuria and decreased erythrocytes, Hb, Ht, and iron and transferrin levels were observed only in RR and/or RX genotypes but not in XX genotypes. The percentage of runners with hematuria, leukocyturia, iron deficiency, creatinine, myoglobin, and bilirubin imbalance was higher in RR compared to XX genotypes. ACTN3 polymorphism is associated with iron metabolism and hematological responses after endurance exercise. Despite these results being based on a small sample, they highlight a protective role of the XX genotype on hematological and renal changes induced by long-distance exercise. Therefore, these findings should be further replicated. Frontiers Media S.A. 2019-06-11 /pmc/articles/PMC6580990/ /pubmed/31244673 http://dx.doi.org/10.3389/fphys.2019.00697 Text en Copyright © 2019 Sierra, Oliveira, Silva, Lima, Benetti, Kiss, Sierra, Ghorayeb, Seto, Pesquero and Cury-Boaventura. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Sierra, Ana Paula Renno
Oliveira, Rodrigo Assunção
Silva, Elton Dias
Lima, Giscard Humberto Oliveira
Benetti, Marino Pereira
Kiss, Maria Augusta Pedanti
Sierra, Carlos Anibal
Ghorayeb, Nabil
Seto, Jane T.
Pesquero, João Bosco
Cury-Boaventura, Maria Fernanda
Association Between Hematological Parameters and Iron Metabolism Response After Marathon Race and ACTN3 Genotype
title Association Between Hematological Parameters and Iron Metabolism Response After Marathon Race and ACTN3 Genotype
title_full Association Between Hematological Parameters and Iron Metabolism Response After Marathon Race and ACTN3 Genotype
title_fullStr Association Between Hematological Parameters and Iron Metabolism Response After Marathon Race and ACTN3 Genotype
title_full_unstemmed Association Between Hematological Parameters and Iron Metabolism Response After Marathon Race and ACTN3 Genotype
title_short Association Between Hematological Parameters and Iron Metabolism Response After Marathon Race and ACTN3 Genotype
title_sort association between hematological parameters and iron metabolism response after marathon race and actn3 genotype
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580990/
https://www.ncbi.nlm.nih.gov/pubmed/31244673
http://dx.doi.org/10.3389/fphys.2019.00697
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