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Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction

We aimed to evaluate the changes in plasma membrane Ca(2+)-ATPase (PMCA) and sarcoendoplasmic reticulum CA(2+)-ATPase (SERCA-2) according to the antepartal magnesium sulfate (MgSO(4)) administration in the placentas from pregnancies with pre-eclampsia (PE) or fetal growth restriction (FGR). Pregnant...

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Autores principales: Cha, Hyun-Hwa, Hwang, Jae-Ryoung, Sung, Ji Hee, Choi, Suk-Joo, Oh, Soo-young, Roh, Cheong-Rae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581081/
https://www.ncbi.nlm.nih.gov/pubmed/30415221
http://dx.doi.org/10.1136/jim-2018-000844
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author Cha, Hyun-Hwa
Hwang, Jae-Ryoung
Sung, Ji Hee
Choi, Suk-Joo
Oh, Soo-young
Roh, Cheong-Rae
author_facet Cha, Hyun-Hwa
Hwang, Jae-Ryoung
Sung, Ji Hee
Choi, Suk-Joo
Oh, Soo-young
Roh, Cheong-Rae
author_sort Cha, Hyun-Hwa
collection PubMed
description We aimed to evaluate the changes in plasma membrane Ca(2+)-ATPase (PMCA) and sarcoendoplasmic reticulum CA(2+)-ATPase (SERCA-2) according to the antepartal magnesium sulfate (MgSO(4)) administration in the placentas from pregnancies with pre-eclampsia (PE) or fetal growth restriction (FGR). Pregnant women were classified as follows: (group 1) pregnancies without PE or FGR (n=16), (group 2) pregnancies with PE or FGR but without MgSO(4) administration (n=14), and (group 3) pregnancies with PE or FGR and with MgSO(4) administration (n=28). We observed the localization of PMCA and SERCA-2 in placentas and compared its expression among 3 groups. And we observed its expression in BeWo cells following treatment with MgSO(4) and CoCl(2). PMCA staining was more observed in the basal membrane, whereas SERCA-2 staining was observed predominantly under the microvillous membrane. SERCA-2 expression was significantly increased in group 3 compared with that in group 1. Considering the gestational age at delivery, PMCA expression was increased in group 2 and group 3 compared with that in group 1 after 36 weeks of gestation. SERCA-2 was increased in group 3, but not in group 2 compared with that in group 1 after 36 weeks of gestation. In BeWo cells, MgSO(4) treatment increased PMCA and SERCA-2 expression. PMCA expression was influenced by gestational age at delivery, and SERCA-2 expression was increased in the presence of PE and antepartal MgSO(4) administration. This indicates that antepartal MgSO(4) administration has a greater influence on SERCA-2 than PMCA.
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spelling pubmed-65810812019-07-02 Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction Cha, Hyun-Hwa Hwang, Jae-Ryoung Sung, Ji Hee Choi, Suk-Joo Oh, Soo-young Roh, Cheong-Rae J Investig Med Original Research We aimed to evaluate the changes in plasma membrane Ca(2+)-ATPase (PMCA) and sarcoendoplasmic reticulum CA(2+)-ATPase (SERCA-2) according to the antepartal magnesium sulfate (MgSO(4)) administration in the placentas from pregnancies with pre-eclampsia (PE) or fetal growth restriction (FGR). Pregnant women were classified as follows: (group 1) pregnancies without PE or FGR (n=16), (group 2) pregnancies with PE or FGR but without MgSO(4) administration (n=14), and (group 3) pregnancies with PE or FGR and with MgSO(4) administration (n=28). We observed the localization of PMCA and SERCA-2 in placentas and compared its expression among 3 groups. And we observed its expression in BeWo cells following treatment with MgSO(4) and CoCl(2). PMCA staining was more observed in the basal membrane, whereas SERCA-2 staining was observed predominantly under the microvillous membrane. SERCA-2 expression was significantly increased in group 3 compared with that in group 1. Considering the gestational age at delivery, PMCA expression was increased in group 2 and group 3 compared with that in group 1 after 36 weeks of gestation. SERCA-2 was increased in group 3, but not in group 2 compared with that in group 1 after 36 weeks of gestation. In BeWo cells, MgSO(4) treatment increased PMCA and SERCA-2 expression. PMCA expression was influenced by gestational age at delivery, and SERCA-2 expression was increased in the presence of PE and antepartal MgSO(4) administration. This indicates that antepartal MgSO(4) administration has a greater influence on SERCA-2 than PMCA. BMJ Publishing Group 2019-02 2018-11-09 /pmc/articles/PMC6581081/ /pubmed/30415221 http://dx.doi.org/10.1136/jim-2018-000844 Text en © American Federation for Medical Research 2019. Re-use permitted under CC BY-NC. No commercial re-use. Published by BMJ. This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, an indication of whether changes were made, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Original Research
Cha, Hyun-Hwa
Hwang, Jae-Ryoung
Sung, Ji Hee
Choi, Suk-Joo
Oh, Soo-young
Roh, Cheong-Rae
Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction
title Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction
title_full Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction
title_fullStr Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction
title_full_unstemmed Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction
title_short Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction
title_sort changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581081/
https://www.ncbi.nlm.nih.gov/pubmed/30415221
http://dx.doi.org/10.1136/jim-2018-000844
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