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MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs)
MOTIVATION: Metagenomics is a powerful tool for assaying the DNA from every genome present in an environment. Recent advances in bioinformatics have enabled the rapid assembly of near-complete metagenome-assembled genomes (MAGs), and there is a need for reproducible pipelines that can annotate and c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581432/ https://www.ncbi.nlm.nih.gov/pubmed/30418481 http://dx.doi.org/10.1093/bioinformatics/bty905 |
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author | Stewart, Robert D Auffret, Marc D Snelling, Timothy J Roehe, Rainer Watson, Mick |
author_facet | Stewart, Robert D Auffret, Marc D Snelling, Timothy J Roehe, Rainer Watson, Mick |
author_sort | Stewart, Robert D |
collection | PubMed |
description | MOTIVATION: Metagenomics is a powerful tool for assaying the DNA from every genome present in an environment. Recent advances in bioinformatics have enabled the rapid assembly of near-complete metagenome-assembled genomes (MAGs), and there is a need for reproducible pipelines that can annotate and characterize thousands of genomes simultaneously, to enable identification and functional characterization. RESULTS: Here we present MAGpy, a scalable and reproducible pipeline that takes multiple genome assemblies as FASTA and compares them to several public databases, checks quality, suggests a taxonomy and draws a phylogenetic tree. AVAILABILITY AND IMPLEMENTATION: MAGpy is available on github: https://github.com/WatsonLab/MAGpy. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online. |
format | Online Article Text |
id | pubmed-6581432 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-65814322019-06-21 MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs) Stewart, Robert D Auffret, Marc D Snelling, Timothy J Roehe, Rainer Watson, Mick Bioinformatics Applications Notes MOTIVATION: Metagenomics is a powerful tool for assaying the DNA from every genome present in an environment. Recent advances in bioinformatics have enabled the rapid assembly of near-complete metagenome-assembled genomes (MAGs), and there is a need for reproducible pipelines that can annotate and characterize thousands of genomes simultaneously, to enable identification and functional characterization. RESULTS: Here we present MAGpy, a scalable and reproducible pipeline that takes multiple genome assemblies as FASTA and compares them to several public databases, checks quality, suggests a taxonomy and draws a phylogenetic tree. AVAILABILITY AND IMPLEMENTATION: MAGpy is available on github: https://github.com/WatsonLab/MAGpy. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online. Oxford University Press 2019-06 2018-11-10 /pmc/articles/PMC6581432/ /pubmed/30418481 http://dx.doi.org/10.1093/bioinformatics/bty905 Text en © The Author(s) 2018. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Applications Notes Stewart, Robert D Auffret, Marc D Snelling, Timothy J Roehe, Rainer Watson, Mick MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs) |
title | MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs) |
title_full | MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs) |
title_fullStr | MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs) |
title_full_unstemmed | MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs) |
title_short | MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs) |
title_sort | magpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (mags) |
topic | Applications Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581432/ https://www.ncbi.nlm.nih.gov/pubmed/30418481 http://dx.doi.org/10.1093/bioinformatics/bty905 |
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