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MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs)

MOTIVATION: Metagenomics is a powerful tool for assaying the DNA from every genome present in an environment. Recent advances in bioinformatics have enabled the rapid assembly of near-complete metagenome-assembled genomes (MAGs), and there is a need for reproducible pipelines that can annotate and c...

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Detalles Bibliográficos
Autores principales: Stewart, Robert D, Auffret, Marc D, Snelling, Timothy J, Roehe, Rainer, Watson, Mick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581432/
https://www.ncbi.nlm.nih.gov/pubmed/30418481
http://dx.doi.org/10.1093/bioinformatics/bty905
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author Stewart, Robert D
Auffret, Marc D
Snelling, Timothy J
Roehe, Rainer
Watson, Mick
author_facet Stewart, Robert D
Auffret, Marc D
Snelling, Timothy J
Roehe, Rainer
Watson, Mick
author_sort Stewart, Robert D
collection PubMed
description MOTIVATION: Metagenomics is a powerful tool for assaying the DNA from every genome present in an environment. Recent advances in bioinformatics have enabled the rapid assembly of near-complete metagenome-assembled genomes (MAGs), and there is a need for reproducible pipelines that can annotate and characterize thousands of genomes simultaneously, to enable identification and functional characterization. RESULTS: Here we present MAGpy, a scalable and reproducible pipeline that takes multiple genome assemblies as FASTA and compares them to several public databases, checks quality, suggests a taxonomy and draws a phylogenetic tree. AVAILABILITY AND IMPLEMENTATION: MAGpy is available on github: https://github.com/WatsonLab/MAGpy. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
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spelling pubmed-65814322019-06-21 MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs) Stewart, Robert D Auffret, Marc D Snelling, Timothy J Roehe, Rainer Watson, Mick Bioinformatics Applications Notes MOTIVATION: Metagenomics is a powerful tool for assaying the DNA from every genome present in an environment. Recent advances in bioinformatics have enabled the rapid assembly of near-complete metagenome-assembled genomes (MAGs), and there is a need for reproducible pipelines that can annotate and characterize thousands of genomes simultaneously, to enable identification and functional characterization. RESULTS: Here we present MAGpy, a scalable and reproducible pipeline that takes multiple genome assemblies as FASTA and compares them to several public databases, checks quality, suggests a taxonomy and draws a phylogenetic tree. AVAILABILITY AND IMPLEMENTATION: MAGpy is available on github: https://github.com/WatsonLab/MAGpy. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online. Oxford University Press 2019-06 2018-11-10 /pmc/articles/PMC6581432/ /pubmed/30418481 http://dx.doi.org/10.1093/bioinformatics/bty905 Text en © The Author(s) 2018. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Applications Notes
Stewart, Robert D
Auffret, Marc D
Snelling, Timothy J
Roehe, Rainer
Watson, Mick
MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs)
title MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs)
title_full MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs)
title_fullStr MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs)
title_full_unstemmed MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs)
title_short MAGpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (MAGs)
title_sort magpy: a reproducible pipeline for the downstream analysis of metagenome-assembled genomes (mags)
topic Applications Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581432/
https://www.ncbi.nlm.nih.gov/pubmed/30418481
http://dx.doi.org/10.1093/bioinformatics/bty905
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