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The mechanism and control of Jagged1 expression in Sertoli cells
The regulation of Sertoli cells by some hormones and signaling factors is important for normal spermatogenesis. Notch signaling is considered to be necessary for normal spermatogenesis in mouse. In this study, we revealed two new facts about Sertoli cells by western blotting experiments on different...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society for Regenerative Medicine
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581826/ https://www.ncbi.nlm.nih.gov/pubmed/31245476 http://dx.doi.org/10.1016/j.reth.2016.02.005 |
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author | Okada, Ryu Hara, Taro Sato, Tomomi Kojima, Nobuhiko Nishina, Yukio |
author_facet | Okada, Ryu Hara, Taro Sato, Tomomi Kojima, Nobuhiko Nishina, Yukio |
author_sort | Okada, Ryu |
collection | PubMed |
description | The regulation of Sertoli cells by some hormones and signaling factors is important for normal spermatogenesis. Notch signaling is considered to be necessary for normal spermatogenesis in mouse. In this study, we revealed two new facts about Sertoli cells by western blotting experiments on different types of primary cells and microdissected tubules. The first is that Sertoli cells express the Jagged1 ligand in mice testes. The second is that the expression level of Jagged1 oscillates in the seminiferous epithelial cycle. Therefore, we inferred that Jagged1 in Sertoli cells contributes to the Notch signaling involved in spermatogenesis. Furthermore, we examined the regulation of Jagged1 expression and found that Jagged1 expression was suppressed by cAMP signaling and was promoted by TNF-α signaling in Sertoli cells. When cAMP and TNF-α were simultaneously added to Sertoli cells, Jagged1 expression was suppressed. Therefore, cAMP signaling dominates Jagged1 expression over TNF-α signaling. These results suggest that cAMP signaling may cause the periodicity of Jagged1 expression in the seminiferous epithelial cycle, and controlling Jagged1 expression by adding TNF-α or cAMP may contribute to normal spermatogenesis in vitro. |
format | Online Article Text |
id | pubmed-6581826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Japanese Society for Regenerative Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-65818262019-06-26 The mechanism and control of Jagged1 expression in Sertoli cells Okada, Ryu Hara, Taro Sato, Tomomi Kojima, Nobuhiko Nishina, Yukio Regen Ther Original Article The regulation of Sertoli cells by some hormones and signaling factors is important for normal spermatogenesis. Notch signaling is considered to be necessary for normal spermatogenesis in mouse. In this study, we revealed two new facts about Sertoli cells by western blotting experiments on different types of primary cells and microdissected tubules. The first is that Sertoli cells express the Jagged1 ligand in mice testes. The second is that the expression level of Jagged1 oscillates in the seminiferous epithelial cycle. Therefore, we inferred that Jagged1 in Sertoli cells contributes to the Notch signaling involved in spermatogenesis. Furthermore, we examined the regulation of Jagged1 expression and found that Jagged1 expression was suppressed by cAMP signaling and was promoted by TNF-α signaling in Sertoli cells. When cAMP and TNF-α were simultaneously added to Sertoli cells, Jagged1 expression was suppressed. Therefore, cAMP signaling dominates Jagged1 expression over TNF-α signaling. These results suggest that cAMP signaling may cause the periodicity of Jagged1 expression in the seminiferous epithelial cycle, and controlling Jagged1 expression by adding TNF-α or cAMP may contribute to normal spermatogenesis in vitro. Japanese Society for Regenerative Medicine 2016-03-22 /pmc/articles/PMC6581826/ /pubmed/31245476 http://dx.doi.org/10.1016/j.reth.2016.02.005 Text en © 2016, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Okada, Ryu Hara, Taro Sato, Tomomi Kojima, Nobuhiko Nishina, Yukio The mechanism and control of Jagged1 expression in Sertoli cells |
title | The mechanism and control of Jagged1 expression in Sertoli cells |
title_full | The mechanism and control of Jagged1 expression in Sertoli cells |
title_fullStr | The mechanism and control of Jagged1 expression in Sertoli cells |
title_full_unstemmed | The mechanism and control of Jagged1 expression in Sertoli cells |
title_short | The mechanism and control of Jagged1 expression in Sertoli cells |
title_sort | mechanism and control of jagged1 expression in sertoli cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581826/ https://www.ncbi.nlm.nih.gov/pubmed/31245476 http://dx.doi.org/10.1016/j.reth.2016.02.005 |
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