Cargando…
Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor
Growth factor receptor-bound protein 2 (Grb2) is an adaptor protein involved in the signal transduction pathways. This dataset enlists proteins which interact with Grb2 in the presence and absence of a mitogenic stimulus. Grb2 expressing HEK293 cells were cultured in light and heavy labeled SILAC me...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582235/ https://www.ncbi.nlm.nih.gov/pubmed/31245515 http://dx.doi.org/10.1016/j.dib.2019.104082 |
_version_ | 1783428282085015552 |
---|---|
author | Duggal, Shweta Midha, Mukul Kumar Kumar, Ajay Rao, Kanury V.S. |
author_facet | Duggal, Shweta Midha, Mukul Kumar Kumar, Ajay Rao, Kanury V.S. |
author_sort | Duggal, Shweta |
collection | PubMed |
description | Growth factor receptor-bound protein 2 (Grb2) is an adaptor protein involved in the signal transduction pathways. This dataset enlists proteins which interact with Grb2 in the presence and absence of a mitogenic stimulus. Grb2 expressing HEK293 cells were cultured in light and heavy labeled SILAC media. Normal lysine and arginine were incorporated as light labels while 8 and 10 Da heavier labels of respective isotopes were used for heavy labeling. While light labeled cells were used to enrich basal Grb2 interactome, the heavy labeled cells were stimulated in presence of epidermal growth factor (EGF) to investigate the altered Grb2 interactome dynamics. Equal number of EGF stimulated and non-stimulated cells was pooled, lysed and subjected to affinity purification coupled to mass spectrometry (AP-MS). The variety of Grb2 protein partners changed as a consequence of EGF stimulation. Additionally, SILAC labeling helped in quantitative estimation of altered association of a few interactors with the bait protein. Data are available via PRIDE repository with the dataset identifier PXD012957 (https://www.ebi.ac.uk/pride/archive/projects/PXD012957). |
format | Online Article Text |
id | pubmed-6582235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-65822352019-06-26 Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor Duggal, Shweta Midha, Mukul Kumar Kumar, Ajay Rao, Kanury V.S. Data Brief Proteomics Growth factor receptor-bound protein 2 (Grb2) is an adaptor protein involved in the signal transduction pathways. This dataset enlists proteins which interact with Grb2 in the presence and absence of a mitogenic stimulus. Grb2 expressing HEK293 cells were cultured in light and heavy labeled SILAC media. Normal lysine and arginine were incorporated as light labels while 8 and 10 Da heavier labels of respective isotopes were used for heavy labeling. While light labeled cells were used to enrich basal Grb2 interactome, the heavy labeled cells were stimulated in presence of epidermal growth factor (EGF) to investigate the altered Grb2 interactome dynamics. Equal number of EGF stimulated and non-stimulated cells was pooled, lysed and subjected to affinity purification coupled to mass spectrometry (AP-MS). The variety of Grb2 protein partners changed as a consequence of EGF stimulation. Additionally, SILAC labeling helped in quantitative estimation of altered association of a few interactors with the bait protein. Data are available via PRIDE repository with the dataset identifier PXD012957 (https://www.ebi.ac.uk/pride/archive/projects/PXD012957). Elsevier 2019-05-30 /pmc/articles/PMC6582235/ /pubmed/31245515 http://dx.doi.org/10.1016/j.dib.2019.104082 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Proteomics Duggal, Shweta Midha, Mukul Kumar Kumar, Ajay Rao, Kanury V.S. Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor |
title | Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor |
title_full | Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor |
title_fullStr | Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor |
title_full_unstemmed | Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor |
title_short | Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor |
title_sort | outlining the grb2 interactome data and its interacting partners in hek293 cells in absence and presence of epidermal growth factor |
topic | Proteomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582235/ https://www.ncbi.nlm.nih.gov/pubmed/31245515 http://dx.doi.org/10.1016/j.dib.2019.104082 |
work_keys_str_mv | AT duggalshweta outliningthegrb2interactomedataanditsinteractingpartnersinhek293cellsinabsenceandpresenceofepidermalgrowthfactor AT midhamukulkumar outliningthegrb2interactomedataanditsinteractingpartnersinhek293cellsinabsenceandpresenceofepidermalgrowthfactor AT kumarajay outliningthegrb2interactomedataanditsinteractingpartnersinhek293cellsinabsenceandpresenceofepidermalgrowthfactor AT raokanuryvs outliningthegrb2interactomedataanditsinteractingpartnersinhek293cellsinabsenceandpresenceofepidermalgrowthfactor |