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Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells Provides A Protective Microenvironment at The Feto-Maternal Interface: Improved Pregnancy Outcome in Abortion-Prone Mice
OBJECTIVE: Dendritic cells (DCs) as major regulators of the immune response in the decidua play a pivotal role in establishment and maintenance of pregnancy. Immunological disorders are considered to be the main causes of unexplained recurrent spontaneous abortions (RSAs). Recently, we reported that...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582417/ https://www.ncbi.nlm.nih.gov/pubmed/31210433 http://dx.doi.org/10.22074/cellj.2019.6239 |
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author | Eskandarian, Maryam Moazzeni, Seyed Mohammad |
author_facet | Eskandarian, Maryam Moazzeni, Seyed Mohammad |
author_sort | Eskandarian, Maryam |
collection | PubMed |
description | OBJECTIVE: Dendritic cells (DCs) as major regulators of the immune response in the decidua play a pivotal role in establishment and maintenance of pregnancy. Immunological disorders are considered to be the main causes of unexplained recurrent spontaneous abortions (RSAs). Recently, we reported that mesenchymal stem cells (MSCs) therapy could improve fetal survival and reduce the abortion rate in abortion-prone mice, although the precise mechanisms of this action are poorly understood. Since MSCs have been shown to exert immunomodulatory effects on the immune cells, especially DCs, this study was performed to investigate the capability of MSCs to modulate the frequency, maturation state, and phenotype of uterine DCs (uDCs) as a potential mechanism for the improvement of pregnancy outcome. MATERIALS AND METHODS: In this experimental study, adipose-derived MSCs were intraperitoneally administered to abortion-prone pregnant mice on the fourth day of gestation. On the day 13.5 of pregnancy, after the determination of abortion rates, the frequency, phenotype, and maturation state of uDCs were analyzed using flow cytometry. RESULTS: Our results indicated that the administration of MSCs, at the implantation window, could significantly decrease the abortion rate and besides, increase the frequency of uDCs. MSCs administration also remarkably decreased the expression of DCs maturation markers (MHC-II, CD86, and CD40) on uDCs. However, we did not find any difference in the expression of CD11b on uDCs in MSCs-treated compared to control mice. CONCLUSION: Regarding the mutual role of uDCs in establishment of a particular immunological state required for appropriate implantation, proper maternal immune responses and development of successful pregnancy, it seems that the modulation of uDCs by MSCs could be considered as one of the main mechanisms responsible for the positive effect of MSCs on treatment of RSA. |
format | Online Article Text |
id | pubmed-6582417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-65824172019-09-01 Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells Provides A Protective Microenvironment at The Feto-Maternal Interface: Improved Pregnancy Outcome in Abortion-Prone Mice Eskandarian, Maryam Moazzeni, Seyed Mohammad Cell J Original Article OBJECTIVE: Dendritic cells (DCs) as major regulators of the immune response in the decidua play a pivotal role in establishment and maintenance of pregnancy. Immunological disorders are considered to be the main causes of unexplained recurrent spontaneous abortions (RSAs). Recently, we reported that mesenchymal stem cells (MSCs) therapy could improve fetal survival and reduce the abortion rate in abortion-prone mice, although the precise mechanisms of this action are poorly understood. Since MSCs have been shown to exert immunomodulatory effects on the immune cells, especially DCs, this study was performed to investigate the capability of MSCs to modulate the frequency, maturation state, and phenotype of uterine DCs (uDCs) as a potential mechanism for the improvement of pregnancy outcome. MATERIALS AND METHODS: In this experimental study, adipose-derived MSCs were intraperitoneally administered to abortion-prone pregnant mice on the fourth day of gestation. On the day 13.5 of pregnancy, after the determination of abortion rates, the frequency, phenotype, and maturation state of uDCs were analyzed using flow cytometry. RESULTS: Our results indicated that the administration of MSCs, at the implantation window, could significantly decrease the abortion rate and besides, increase the frequency of uDCs. MSCs administration also remarkably decreased the expression of DCs maturation markers (MHC-II, CD86, and CD40) on uDCs. However, we did not find any difference in the expression of CD11b on uDCs in MSCs-treated compared to control mice. CONCLUSION: Regarding the mutual role of uDCs in establishment of a particular immunological state required for appropriate implantation, proper maternal immune responses and development of successful pregnancy, it seems that the modulation of uDCs by MSCs could be considered as one of the main mechanisms responsible for the positive effect of MSCs on treatment of RSA. Royan Institute 2019 2019-06-15 /pmc/articles/PMC6582417/ /pubmed/31210433 http://dx.doi.org/10.22074/cellj.2019.6239 Text en The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited. http://creativecommons.org/licenses/by/3/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Eskandarian, Maryam Moazzeni, Seyed Mohammad Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells Provides A Protective Microenvironment at The Feto-Maternal Interface: Improved Pregnancy Outcome in Abortion-Prone Mice |
title | Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells
Provides A Protective Microenvironment at The
Feto-Maternal Interface: Improved Pregnancy
Outcome in Abortion-Prone Mice |
title_full | Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells
Provides A Protective Microenvironment at The
Feto-Maternal Interface: Improved Pregnancy
Outcome in Abortion-Prone Mice |
title_fullStr | Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells
Provides A Protective Microenvironment at The
Feto-Maternal Interface: Improved Pregnancy
Outcome in Abortion-Prone Mice |
title_full_unstemmed | Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells
Provides A Protective Microenvironment at The
Feto-Maternal Interface: Improved Pregnancy
Outcome in Abortion-Prone Mice |
title_short | Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells
Provides A Protective Microenvironment at The
Feto-Maternal Interface: Improved Pregnancy
Outcome in Abortion-Prone Mice |
title_sort | uterine dendritic cells modulation by mesenchymal stem cells
provides a protective microenvironment at the
feto-maternal interface: improved pregnancy
outcome in abortion-prone mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582417/ https://www.ncbi.nlm.nih.gov/pubmed/31210433 http://dx.doi.org/10.22074/cellj.2019.6239 |
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