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Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry

A sensitive and accurate simultaneous continuous analysis for six arsenic species including arsenobetaine (AsB), arsenocholine (AsC), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenite (As(III)), and arsenate (As(V)) has been developed by high performance liquid chromatography with i...

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Autores principales: Hou, Hongfang, Cui, Wanjing, Xu, Qing, Tao, Zhanhui, Guo, Yafei, Deng, Tianlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582848/
https://www.ncbi.nlm.nih.gov/pubmed/31275389
http://dx.doi.org/10.1155/2019/3280840
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author Hou, Hongfang
Cui, Wanjing
Xu, Qing
Tao, Zhanhui
Guo, Yafei
Deng, Tianlong
author_facet Hou, Hongfang
Cui, Wanjing
Xu, Qing
Tao, Zhanhui
Guo, Yafei
Deng, Tianlong
author_sort Hou, Hongfang
collection PubMed
description A sensitive and accurate simultaneous continuous analysis for six arsenic species including arsenobetaine (AsB), arsenocholine (AsC), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenite (As(III)), and arsenate (As(V)) has been developed by high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). An anion-exchange column of Hamilton PRP-X100 (Switzerland) was applied for separation of the six arsenic species with gradient elution of 1.25 mmol/L Na(2)HPO(4) and 11.0 mmol/L KH(2)PO(4) as the mobile phase A and 2.5 mmol/L Na(2)HPO(4) and 22.0 mmol/L KH(2)PO(4) as the mobile phase B. The linearity ranges for AsB, AsC, MMA, DMA, As(III), and As(V) were between 0.5 and 50.0 μg/L, and the detection limits of the six arsenic species were all within 0.01–0.35 ng/L. The relative standard deviations (RSDs) were within 2.26–3.68% and the recovery rates of samples ranged from 95 to 103%. The proposed method was applied for the arsenic speciation analysis of sediment pore-water samples, which were taken from the supernatant after centrifugation and filtration.
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spelling pubmed-65828482019-07-03 Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry Hou, Hongfang Cui, Wanjing Xu, Qing Tao, Zhanhui Guo, Yafei Deng, Tianlong Int J Anal Chem Research Article A sensitive and accurate simultaneous continuous analysis for six arsenic species including arsenobetaine (AsB), arsenocholine (AsC), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenite (As(III)), and arsenate (As(V)) has been developed by high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). An anion-exchange column of Hamilton PRP-X100 (Switzerland) was applied for separation of the six arsenic species with gradient elution of 1.25 mmol/L Na(2)HPO(4) and 11.0 mmol/L KH(2)PO(4) as the mobile phase A and 2.5 mmol/L Na(2)HPO(4) and 22.0 mmol/L KH(2)PO(4) as the mobile phase B. The linearity ranges for AsB, AsC, MMA, DMA, As(III), and As(V) were between 0.5 and 50.0 μg/L, and the detection limits of the six arsenic species were all within 0.01–0.35 ng/L. The relative standard deviations (RSDs) were within 2.26–3.68% and the recovery rates of samples ranged from 95 to 103%. The proposed method was applied for the arsenic speciation analysis of sediment pore-water samples, which were taken from the supernatant after centrifugation and filtration. Hindawi 2019-06-04 /pmc/articles/PMC6582848/ /pubmed/31275389 http://dx.doi.org/10.1155/2019/3280840 Text en Copyright © 2019 Hongfang Hou et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hou, Hongfang
Cui, Wanjing
Xu, Qing
Tao, Zhanhui
Guo, Yafei
Deng, Tianlong
Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_full Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_fullStr Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_full_unstemmed Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_short Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_sort arsenic species analysis at trace level by high performance liquid chromatography with inductively coupled plasma mass spectrometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582848/
https://www.ncbi.nlm.nih.gov/pubmed/31275389
http://dx.doi.org/10.1155/2019/3280840
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