Cargando…

The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats

Mulberry water extracts (MB) and silk amino acids (SA) are reported to improve oxidative stress and inflammation, respectively. We hypothesized whether the mixture of mulberry water extracts and silk amino acids can alleviate nonalcoholic fatty liver disease (NAFLD) induced by high fat diets. Male S...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Sunmin, Zhang, Ting, Qiu, Jing Yi, Wu, Xuangao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582910/
https://www.ncbi.nlm.nih.gov/pubmed/31275421
http://dx.doi.org/10.1155/2019/8063121
_version_ 1783428424669331456
author Park, Sunmin
Zhang, Ting
Qiu, Jing Yi
Wu, Xuangao
author_facet Park, Sunmin
Zhang, Ting
Qiu, Jing Yi
Wu, Xuangao
author_sort Park, Sunmin
collection PubMed
description Mulberry water extracts (MB) and silk amino acids (SA) are reported to improve oxidative stress and inflammation, respectively. We hypothesized whether the mixture of mulberry water extracts and silk amino acids can alleviate nonalcoholic fatty liver disease (NAFLD) induced by high fat diets. Male Sprague Dawley rats were orally provided with high fat diets containing different ratios of MB and SA (1:3, MS1:3, or 1:5, MS1:5) or cellulose (the disease-control) for 12 weeks. Rats had 200 or 600 mg/kg bw of MS1:3 and MS1:5 (MS1:3-L, MS1:3-H; MS1:5-L, and MS1:5-H). Rats in the normal-control group were fed the 20% fat diet with cellulose. Disease-control rats exhibited much greater triglyceride (TG) deposition in the liver than the normal-control rats along with increased body weight gain, visceral fat mass, serum concentrations of cholesterol, triglyceride and nonesterified fatty acid (NEFA), and insulin resistance. Disease-control rats also had liver damage with increased oxidative stress and inflammation compared to the normal-control rats. MS1:3-H and MS1:5-H were found to have greater hepatic glycogen accumulation and decreased hepatic TG, insulin resistance, and dyslipidemia, with MS1:5-H being similar to the normal-control. MS1:3-H alleviated oxidative stress with lower hepatic lipid peroxide compared to MS1:5-H whereas MS1:5-H ameliorated inflammation and hepatocyte damage better than MS1:3-H. Both MS1:3-H and MS1:5-H potentiated hepatic insulin signaling (pAkt⟶pACC) and reduced the mRNA expression of TG synthesis genes mRNA (FAS and SREBP-1c). In the gut microbiome MS1:3-H elevated the ratio of Bacteroidales to Clostridiales in the cecum better than MS1:5-H but MS1:5-H reduced the proinflammatory Turicibacterales. In conclusion, both MS1:3-H and MS1:5-H prevented liver damage induced by high fat diets, mainly by suppressing oxidative stress and inflammation, respectively. MS1:3 and MS1:5 might be used as therapeutic agent for NAFLD.
format Online
Article
Text
id pubmed-6582910
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-65829102019-07-03 The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats Park, Sunmin Zhang, Ting Qiu, Jing Yi Wu, Xuangao Evid Based Complement Alternat Med Research Article Mulberry water extracts (MB) and silk amino acids (SA) are reported to improve oxidative stress and inflammation, respectively. We hypothesized whether the mixture of mulberry water extracts and silk amino acids can alleviate nonalcoholic fatty liver disease (NAFLD) induced by high fat diets. Male Sprague Dawley rats were orally provided with high fat diets containing different ratios of MB and SA (1:3, MS1:3, or 1:5, MS1:5) or cellulose (the disease-control) for 12 weeks. Rats had 200 or 600 mg/kg bw of MS1:3 and MS1:5 (MS1:3-L, MS1:3-H; MS1:5-L, and MS1:5-H). Rats in the normal-control group were fed the 20% fat diet with cellulose. Disease-control rats exhibited much greater triglyceride (TG) deposition in the liver than the normal-control rats along with increased body weight gain, visceral fat mass, serum concentrations of cholesterol, triglyceride and nonesterified fatty acid (NEFA), and insulin resistance. Disease-control rats also had liver damage with increased oxidative stress and inflammation compared to the normal-control rats. MS1:3-H and MS1:5-H were found to have greater hepatic glycogen accumulation and decreased hepatic TG, insulin resistance, and dyslipidemia, with MS1:5-H being similar to the normal-control. MS1:3-H alleviated oxidative stress with lower hepatic lipid peroxide compared to MS1:5-H whereas MS1:5-H ameliorated inflammation and hepatocyte damage better than MS1:3-H. Both MS1:3-H and MS1:5-H potentiated hepatic insulin signaling (pAkt⟶pACC) and reduced the mRNA expression of TG synthesis genes mRNA (FAS and SREBP-1c). In the gut microbiome MS1:3-H elevated the ratio of Bacteroidales to Clostridiales in the cecum better than MS1:5-H but MS1:5-H reduced the proinflammatory Turicibacterales. In conclusion, both MS1:3-H and MS1:5-H prevented liver damage induced by high fat diets, mainly by suppressing oxidative stress and inflammation, respectively. MS1:3 and MS1:5 might be used as therapeutic agent for NAFLD. Hindawi 2019-05-30 /pmc/articles/PMC6582910/ /pubmed/31275421 http://dx.doi.org/10.1155/2019/8063121 Text en Copyright © 2019 Sunmin Park et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Park, Sunmin
Zhang, Ting
Qiu, Jing Yi
Wu, Xuangao
The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats
title The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats
title_full The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats
title_fullStr The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats
title_full_unstemmed The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats
title_short The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats
title_sort combination of mulberry extracts and silk amino acids alleviated high fat diet-induced nonalcoholic hepatic steatosis by improving hepatic insulin signaling and normalizing gut microbiome dysbiosis in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582910/
https://www.ncbi.nlm.nih.gov/pubmed/31275421
http://dx.doi.org/10.1155/2019/8063121
work_keys_str_mv AT parksunmin thecombinationofmulberryextractsandsilkaminoacidsalleviatedhighfatdietinducednonalcoholichepaticsteatosisbyimprovinghepaticinsulinsignalingandnormalizinggutmicrobiomedysbiosisinrats
AT zhangting thecombinationofmulberryextractsandsilkaminoacidsalleviatedhighfatdietinducednonalcoholichepaticsteatosisbyimprovinghepaticinsulinsignalingandnormalizinggutmicrobiomedysbiosisinrats
AT qiujingyi thecombinationofmulberryextractsandsilkaminoacidsalleviatedhighfatdietinducednonalcoholichepaticsteatosisbyimprovinghepaticinsulinsignalingandnormalizinggutmicrobiomedysbiosisinrats
AT wuxuangao thecombinationofmulberryextractsandsilkaminoacidsalleviatedhighfatdietinducednonalcoholichepaticsteatosisbyimprovinghepaticinsulinsignalingandnormalizinggutmicrobiomedysbiosisinrats
AT parksunmin combinationofmulberryextractsandsilkaminoacidsalleviatedhighfatdietinducednonalcoholichepaticsteatosisbyimprovinghepaticinsulinsignalingandnormalizinggutmicrobiomedysbiosisinrats
AT zhangting combinationofmulberryextractsandsilkaminoacidsalleviatedhighfatdietinducednonalcoholichepaticsteatosisbyimprovinghepaticinsulinsignalingandnormalizinggutmicrobiomedysbiosisinrats
AT qiujingyi combinationofmulberryextractsandsilkaminoacidsalleviatedhighfatdietinducednonalcoholichepaticsteatosisbyimprovinghepaticinsulinsignalingandnormalizinggutmicrobiomedysbiosisinrats
AT wuxuangao combinationofmulberryextractsandsilkaminoacidsalleviatedhighfatdietinducednonalcoholichepaticsteatosisbyimprovinghepaticinsulinsignalingandnormalizinggutmicrobiomedysbiosisinrats