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Composite Fasciola hepatica faecal egg sedimentation test for cattle

Options for diagnosing Fasciola hepatica infection in groups of cattle are limited. Increasing the opportunities for herd-level diagnosis is important for disease monitoring, making informed treatment decisions and for flukicide efficacy testing. The sensitivity of a simple sedimentation method base...

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Autores principales: Graham-Brown, John, Williams, Diana J L, Skuce, Philip, Zadoks, Ruth N, Dawes, Stuart, Swales, Harry, Van Dijk, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6583716/
https://www.ncbi.nlm.nih.gov/pubmed/30711919
http://dx.doi.org/10.1136/vr.105128
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author Graham-Brown, John
Williams, Diana J L
Skuce, Philip
Zadoks, Ruth N
Dawes, Stuart
Swales, Harry
Van Dijk, Jan
author_facet Graham-Brown, John
Williams, Diana J L
Skuce, Philip
Zadoks, Ruth N
Dawes, Stuart
Swales, Harry
Van Dijk, Jan
author_sort Graham-Brown, John
collection PubMed
description Options for diagnosing Fasciola hepatica infection in groups of cattle are limited. Increasing the opportunities for herd-level diagnosis is important for disease monitoring, making informed treatment decisions and for flukicide efficacy testing. The sensitivity of a simple sedimentation method based on composite faecal samples for the detection of fluke eggs in cattle was assessed through a combination of experimental and statistical modelling techniques. Initially, a composite sample method previously developed for sheep was used to investigate the sensitivity of composite sample testing compared with individual counts on the same samples in cattle. Following this, an optimised, validated, qualitative (presence-absence) composite sample field test was developed for cattle. Results showed that fluke egg counts obtained from a composite sample are representative of those expected from individual counts. The optimal sampling strategy was determined to be 10 individual 10 g samples (100 g composite sample) from which a 10 g subsample is taken for sedimentation. This method yielded a diagnostic sensitivity of 0.69 (95 per cent CI 0.5 to 0.85). These results demonstrate the validity and usefulness of a composite faecal egg sedimentation method for use in the diagnosis and control of F. hepatica in groups of cattle, with the caveat that a negative test should be followed up with a second test due to limitations relating to test sensitivity.
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spelling pubmed-65837162019-07-05 Composite Fasciola hepatica faecal egg sedimentation test for cattle Graham-Brown, John Williams, Diana J L Skuce, Philip Zadoks, Ruth N Dawes, Stuart Swales, Harry Van Dijk, Jan Vet Rec Paper Options for diagnosing Fasciola hepatica infection in groups of cattle are limited. Increasing the opportunities for herd-level diagnosis is important for disease monitoring, making informed treatment decisions and for flukicide efficacy testing. The sensitivity of a simple sedimentation method based on composite faecal samples for the detection of fluke eggs in cattle was assessed through a combination of experimental and statistical modelling techniques. Initially, a composite sample method previously developed for sheep was used to investigate the sensitivity of composite sample testing compared with individual counts on the same samples in cattle. Following this, an optimised, validated, qualitative (presence-absence) composite sample field test was developed for cattle. Results showed that fluke egg counts obtained from a composite sample are representative of those expected from individual counts. The optimal sampling strategy was determined to be 10 individual 10 g samples (100 g composite sample) from which a 10 g subsample is taken for sedimentation. This method yielded a diagnostic sensitivity of 0.69 (95 per cent CI 0.5 to 0.85). These results demonstrate the validity and usefulness of a composite faecal egg sedimentation method for use in the diagnosis and control of F. hepatica in groups of cattle, with the caveat that a negative test should be followed up with a second test due to limitations relating to test sensitivity. BMJ Publishing Group 2019-05-11 2019-02-02 /pmc/articles/PMC6583716/ /pubmed/30711919 http://dx.doi.org/10.1136/vr.105128 Text en © British Veterinary Association 2019. Re-use permitted under CC BY. Published by BMJ. This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.
spellingShingle Paper
Graham-Brown, John
Williams, Diana J L
Skuce, Philip
Zadoks, Ruth N
Dawes, Stuart
Swales, Harry
Van Dijk, Jan
Composite Fasciola hepatica faecal egg sedimentation test for cattle
title Composite Fasciola hepatica faecal egg sedimentation test for cattle
title_full Composite Fasciola hepatica faecal egg sedimentation test for cattle
title_fullStr Composite Fasciola hepatica faecal egg sedimentation test for cattle
title_full_unstemmed Composite Fasciola hepatica faecal egg sedimentation test for cattle
title_short Composite Fasciola hepatica faecal egg sedimentation test for cattle
title_sort composite fasciola hepatica faecal egg sedimentation test for cattle
topic Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6583716/
https://www.ncbi.nlm.nih.gov/pubmed/30711919
http://dx.doi.org/10.1136/vr.105128
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