Assessment of GC-MS in Detecting Changes in the Levels of Metabolites Using a Spike-in Experiment in Human Plasma

Gas Chromatography coupled with Mass Spectrometry (GC-MS) has been broadly used for the detection of changes in metabolite levels in complex samples. Internal Standards (IS) spiked into a complex background at different concentrations help assess the capability of GC-MS in detecting changes in metab...

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Autores principales: Varghese, Rency S., Poto, Cristina Di, Tu, Chao, Ranjbar, Mohammad R. Nezami, Luo, Yue, Choi, Jiwon, Tadesse, Mahlet G., Ressom, Habtom W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6583805/
https://www.ncbi.nlm.nih.gov/pubmed/31218095
http://dx.doi.org/10.4172/2153-0769.1000175
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author Varghese, Rency S.
Poto, Cristina Di
Tu, Chao
Ranjbar, Mohammad R. Nezami
Luo, Yue
Choi, Jiwon
Tadesse, Mahlet G.
Ressom, Habtom W.
author_facet Varghese, Rency S.
Poto, Cristina Di
Tu, Chao
Ranjbar, Mohammad R. Nezami
Luo, Yue
Choi, Jiwon
Tadesse, Mahlet G.
Ressom, Habtom W.
author_sort Varghese, Rency S.
collection PubMed
description Gas Chromatography coupled with Mass Spectrometry (GC-MS) has been broadly used for the detection of changes in metabolite levels in complex samples. Internal Standards (IS) spiked into a complex background at different concentrations help assess the capability of GC-MS in detecting changes in metabolite levels. This study uses a Latin square design to evaluate the ability of GC-MS in full scan and Single Ion Monitoring (SIM) modes to detect changes among IS spiked into human plasma samples at varying concentrations. Statistical analysis of the data demonstrates the potential of GC-MS to detect true differences over a wide range of concentration levels.
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spelling pubmed-65838052019-06-19 Assessment of GC-MS in Detecting Changes in the Levels of Metabolites Using a Spike-in Experiment in Human Plasma Varghese, Rency S. Poto, Cristina Di Tu, Chao Ranjbar, Mohammad R. Nezami Luo, Yue Choi, Jiwon Tadesse, Mahlet G. Ressom, Habtom W. Metabolomics (Los Angel) Article Gas Chromatography coupled with Mass Spectrometry (GC-MS) has been broadly used for the detection of changes in metabolite levels in complex samples. Internal Standards (IS) spiked into a complex background at different concentrations help assess the capability of GC-MS in detecting changes in metabolite levels. This study uses a Latin square design to evaluate the ability of GC-MS in full scan and Single Ion Monitoring (SIM) modes to detect changes among IS spiked into human plasma samples at varying concentrations. Statistical analysis of the data demonstrates the potential of GC-MS to detect true differences over a wide range of concentration levels. 2016-06-03 2016 /pmc/articles/PMC6583805/ /pubmed/31218095 http://dx.doi.org/10.4172/2153-0769.1000175 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Varghese, Rency S.
Poto, Cristina Di
Tu, Chao
Ranjbar, Mohammad R. Nezami
Luo, Yue
Choi, Jiwon
Tadesse, Mahlet G.
Ressom, Habtom W.
Assessment of GC-MS in Detecting Changes in the Levels of Metabolites Using a Spike-in Experiment in Human Plasma
title Assessment of GC-MS in Detecting Changes in the Levels of Metabolites Using a Spike-in Experiment in Human Plasma
title_full Assessment of GC-MS in Detecting Changes in the Levels of Metabolites Using a Spike-in Experiment in Human Plasma
title_fullStr Assessment of GC-MS in Detecting Changes in the Levels of Metabolites Using a Spike-in Experiment in Human Plasma
title_full_unstemmed Assessment of GC-MS in Detecting Changes in the Levels of Metabolites Using a Spike-in Experiment in Human Plasma
title_short Assessment of GC-MS in Detecting Changes in the Levels of Metabolites Using a Spike-in Experiment in Human Plasma
title_sort assessment of gc-ms in detecting changes in the levels of metabolites using a spike-in experiment in human plasma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6583805/
https://www.ncbi.nlm.nih.gov/pubmed/31218095
http://dx.doi.org/10.4172/2153-0769.1000175
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