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Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis

In insects, the gustatory system has a critical function not only in selecting food and feeding behaviours but also in growth and metabolism. Gustatory receptors play an irreplaceable role in insect gustatory signalling. Trichogramma chilonis is an effective biocontrol agent against agricultural ins...

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Autores principales: Liu, Jianbai, Wu, Han, Yi, Jiequn, Jiang, Dingxin, Zhang, Guren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6583964/
https://www.ncbi.nlm.nih.gov/pubmed/31216287
http://dx.doi.org/10.1371/journal.pone.0217493
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author Liu, Jianbai
Wu, Han
Yi, Jiequn
Jiang, Dingxin
Zhang, Guren
author_facet Liu, Jianbai
Wu, Han
Yi, Jiequn
Jiang, Dingxin
Zhang, Guren
author_sort Liu, Jianbai
collection PubMed
description In insects, the gustatory system has a critical function not only in selecting food and feeding behaviours but also in growth and metabolism. Gustatory receptors play an irreplaceable role in insect gustatory signalling. Trichogramma chilonis is an effective biocontrol agent against agricultural insect pests. However, the molecular mechanism of gustation in T. chilonis remains elusive. In this study, we found that T. chilonis adults had a preference for D-fructose and that D-fructose contributed to prolong longevity and improve fecundity. Then, We also isolated the full-length cDNA encoding candidate gustatory receptor (TchiGR43a) based on the transcriptome data of T. chilonis, and observed that the candidate gustatory receptor gene was expressed from the larval to adult stages. The expression levels of TchiGR43a were similar between female and male. A Xenopus oocyte expression system and two-electrode voltage-clamp recording further verified the function analysis of TchiGR43a. Electrophysiological results showed that TchiGR43a was exclusively tuned to D-fructose. By the studies of behaviour, molecular biology and electrophysiology in T. chilonis, our results lay a basic fundation of further study on the molecular mechanisms of gustatory reception and provide theoretical basis for the nutritional requirement of T. chilonis in biocontrol.
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spelling pubmed-65839642019-06-28 Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis Liu, Jianbai Wu, Han Yi, Jiequn Jiang, Dingxin Zhang, Guren PLoS One Research Article In insects, the gustatory system has a critical function not only in selecting food and feeding behaviours but also in growth and metabolism. Gustatory receptors play an irreplaceable role in insect gustatory signalling. Trichogramma chilonis is an effective biocontrol agent against agricultural insect pests. However, the molecular mechanism of gustation in T. chilonis remains elusive. In this study, we found that T. chilonis adults had a preference for D-fructose and that D-fructose contributed to prolong longevity and improve fecundity. Then, We also isolated the full-length cDNA encoding candidate gustatory receptor (TchiGR43a) based on the transcriptome data of T. chilonis, and observed that the candidate gustatory receptor gene was expressed from the larval to adult stages. The expression levels of TchiGR43a were similar between female and male. A Xenopus oocyte expression system and two-electrode voltage-clamp recording further verified the function analysis of TchiGR43a. Electrophysiological results showed that TchiGR43a was exclusively tuned to D-fructose. By the studies of behaviour, molecular biology and electrophysiology in T. chilonis, our results lay a basic fundation of further study on the molecular mechanisms of gustatory reception and provide theoretical basis for the nutritional requirement of T. chilonis in biocontrol. Public Library of Science 2019-06-19 /pmc/articles/PMC6583964/ /pubmed/31216287 http://dx.doi.org/10.1371/journal.pone.0217493 Text en © 2019 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Liu, Jianbai
Wu, Han
Yi, Jiequn
Jiang, Dingxin
Zhang, Guren
Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis
title Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis
title_full Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis
title_fullStr Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis
title_full_unstemmed Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis
title_short Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis
title_sort identification and functional characterization of d-fructose receptor in an egg parasitoid, trichogramma chilonis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6583964/
https://www.ncbi.nlm.nih.gov/pubmed/31216287
http://dx.doi.org/10.1371/journal.pone.0217493
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