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Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment
As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Evans et al., 2015), that described how we intended to replicate selected experiments from the paper ‘Wnt activity defines colon cancer stem cells and is regulated by the microenvironment’ (Vermeulen et al., 201...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584130/ https://www.ncbi.nlm.nih.gov/pubmed/31215867 http://dx.doi.org/10.7554/eLife.45426 |
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author | Essex, Anthony Pineda, Javier Acharya, Grishma Xin, Hong Evans, James |
author_facet | Essex, Anthony Pineda, Javier Acharya, Grishma Xin, Hong Evans, James |
author_sort | Essex, Anthony |
collection | PubMed |
description | As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Evans et al., 2015), that described how we intended to replicate selected experiments from the paper ‘Wnt activity defines colon cancer stem cells and is regulated by the microenvironment’ (Vermeulen et al., 2010). Here, we report the results. Using three independent primary spheroidal colon cancer cultures that expressed a Wnt reporter construct we observed high Wnt activity was associated with the cell surface markers CD133, CD166, and CD29, but not CD24 and CD44, while the original study found all five markers were correlated with high Wnt activity (Figure 2F; Vermeulen et al., 2010). Clonogenicity was highest in cells with high Wnt activity and clonogenic potential of cells with low Wnt activity were increased by myofibroblast-secreted factors, including HGF. While the effects were in the same direction as the original study (Figure 6D; Vermeulen et al., 2010) whether statistical significance was reached among the different conditions varied. When tested in vivo, we did not find a difference in tumorigenicity between high and low Wnt activity, while the original study found cells with high Wnt activity were more effective in inducing tumors (Figure 7E; Vermeulen et al., 2010). Tumorigenicity, however, was increased with myofibroblast-secreted factors, which was in the same direction as the original study (Figure 7E; Vermeulen et al., 2010), but not statistically significant. Finally, we report meta-analyses for each results where possible. |
format | Online Article Text |
id | pubmed-6584130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65841302019-06-21 Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment Essex, Anthony Pineda, Javier Acharya, Grishma Xin, Hong Evans, James eLife Cancer Biology As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Evans et al., 2015), that described how we intended to replicate selected experiments from the paper ‘Wnt activity defines colon cancer stem cells and is regulated by the microenvironment’ (Vermeulen et al., 2010). Here, we report the results. Using three independent primary spheroidal colon cancer cultures that expressed a Wnt reporter construct we observed high Wnt activity was associated with the cell surface markers CD133, CD166, and CD29, but not CD24 and CD44, while the original study found all five markers were correlated with high Wnt activity (Figure 2F; Vermeulen et al., 2010). Clonogenicity was highest in cells with high Wnt activity and clonogenic potential of cells with low Wnt activity were increased by myofibroblast-secreted factors, including HGF. While the effects were in the same direction as the original study (Figure 6D; Vermeulen et al., 2010) whether statistical significance was reached among the different conditions varied. When tested in vivo, we did not find a difference in tumorigenicity between high and low Wnt activity, while the original study found cells with high Wnt activity were more effective in inducing tumors (Figure 7E; Vermeulen et al., 2010). Tumorigenicity, however, was increased with myofibroblast-secreted factors, which was in the same direction as the original study (Figure 7E; Vermeulen et al., 2010), but not statistically significant. Finally, we report meta-analyses for each results where possible. eLife Sciences Publications, Ltd 2019-06-19 /pmc/articles/PMC6584130/ /pubmed/31215867 http://dx.doi.org/10.7554/eLife.45426 Text en © 2019, Essex et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cancer Biology Essex, Anthony Pineda, Javier Acharya, Grishma Xin, Hong Evans, James Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment |
title | Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment |
title_full | Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment |
title_fullStr | Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment |
title_full_unstemmed | Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment |
title_short | Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment |
title_sort | replication study: wnt activity defines colon cancer stem cells and is regulated by the microenvironment |
topic | Cancer Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584130/ https://www.ncbi.nlm.nih.gov/pubmed/31215867 http://dx.doi.org/10.7554/eLife.45426 |
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