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Biophysical basis underlying dynamic Lck activation visualized by ZapLck FRET biosensor

Lck plays crucial roles in TCR signaling. We developed a new and sensitive FRET biosensor (ZapLck) to visualize Lck kinase activity with high spatiotemporal resolutions in live cells. ZapLck revealed that 62% of Lck signal was preactivated in T-cells. In Lck-deficient JCam T-cells, Lck preactivation...

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Detalles Bibliográficos
Autores principales:	Wan, Rongxue, Wu, Jenny, Ouyang, Mingxing, Lei, Lei, Wei, Jiaming, Peng, Qin, Harrison, Reed, Wu, Yiqian, Cheng, Binbin, Li, Kaitao, Zhu, Cheng, Tang, Liling, Wang, Yingxiao, Lu, Shaoying
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Association for the Advancement of Science 2019
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584686/ https://www.ncbi.nlm.nih.gov/pubmed/31223643 http://dx.doi.org/10.1126/sciadv.aau2001

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