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Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C

The sinusoidal endothelial cells present in the liver (LSECs) are tipically characterized by the presence of pores (fenestrae). During some pathological conditions LSECs undergo “capillarization”, a process characterized by loss of fenestrations and acquisition of a vascular phenotype. In chronic li...

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Autores principales: Baiocchini, Andrea, Del Nonno, Franca, Taibi, Chiara, Visco-Comandini, Ubaldo, D’Offizi, Gianpiero, Piacentini, Mauro, Falasca, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584733/
https://www.ncbi.nlm.nih.gov/pubmed/31217430
http://dx.doi.org/10.1038/s41598-019-45114-1
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author Baiocchini, Andrea
Del Nonno, Franca
Taibi, Chiara
Visco-Comandini, Ubaldo
D’Offizi, Gianpiero
Piacentini, Mauro
Falasca, Laura
author_facet Baiocchini, Andrea
Del Nonno, Franca
Taibi, Chiara
Visco-Comandini, Ubaldo
D’Offizi, Gianpiero
Piacentini, Mauro
Falasca, Laura
author_sort Baiocchini, Andrea
collection PubMed
description The sinusoidal endothelial cells present in the liver (LSECs) are tipically characterized by the presence of pores (fenestrae). During some pathological conditions LSECs undergo “capillarization”, a process characterized by loss of fenestrations and acquisition of a vascular phenotype. In chronic liver disease capillarization has been reported to precede the development of fibrosis. LSECs modification in the setting of HCV infection is currently poorly investigated. Considering that HCV accounts for important changes in hepatocytes and in view of the intimate connection between hepatocytes and LSECs, here we set out to study in great detail the LSECs modifications in individuals with HCV-dependent chronic hepatitis. Electron microscopy analysis, and evaluation of CD32, CD31 and caveolin-1 expression showed that in HCV infection LSECs display major morphological changes but maintain their phenotypical identity. Capillarization was observed only in cases at initial stages of fibrosis. Our findings showed that the severity of LSECs modifications appears to be correlated with hepatocytes damage and fibrosis stage providing novel insight in the pathogenesis of HCV-chronic hepatitis.
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spelling pubmed-65847332019-06-26 Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C Baiocchini, Andrea Del Nonno, Franca Taibi, Chiara Visco-Comandini, Ubaldo D’Offizi, Gianpiero Piacentini, Mauro Falasca, Laura Sci Rep Article The sinusoidal endothelial cells present in the liver (LSECs) are tipically characterized by the presence of pores (fenestrae). During some pathological conditions LSECs undergo “capillarization”, a process characterized by loss of fenestrations and acquisition of a vascular phenotype. In chronic liver disease capillarization has been reported to precede the development of fibrosis. LSECs modification in the setting of HCV infection is currently poorly investigated. Considering that HCV accounts for important changes in hepatocytes and in view of the intimate connection between hepatocytes and LSECs, here we set out to study in great detail the LSECs modifications in individuals with HCV-dependent chronic hepatitis. Electron microscopy analysis, and evaluation of CD32, CD31 and caveolin-1 expression showed that in HCV infection LSECs display major morphological changes but maintain their phenotypical identity. Capillarization was observed only in cases at initial stages of fibrosis. Our findings showed that the severity of LSECs modifications appears to be correlated with hepatocytes damage and fibrosis stage providing novel insight in the pathogenesis of HCV-chronic hepatitis. Nature Publishing Group UK 2019-06-19 /pmc/articles/PMC6584733/ /pubmed/31217430 http://dx.doi.org/10.1038/s41598-019-45114-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Baiocchini, Andrea
Del Nonno, Franca
Taibi, Chiara
Visco-Comandini, Ubaldo
D’Offizi, Gianpiero
Piacentini, Mauro
Falasca, Laura
Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C
title Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C
title_full Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C
title_fullStr Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C
title_full_unstemmed Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C
title_short Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C
title_sort liver sinusoidal endothelial cells (lsecs) modifications in patients with chronic hepatitis c
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584733/
https://www.ncbi.nlm.nih.gov/pubmed/31217430
http://dx.doi.org/10.1038/s41598-019-45114-1
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