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Prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks
AIM: This study aimed to investigate the prevalence of different bacterial species affecting ducks as well as demonstrating the antimicrobial susceptibility and molecular typing of the isolated strains. MATERIALS AND METHODS: A total of 500 samples were randomly collected from different duck farms a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584852/ https://www.ncbi.nlm.nih.gov/pubmed/31327903 http://dx.doi.org/10.14202/vetworld.2019.677-683 |
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author | Eid, Hamza M. Algammal, Abdelazeem M. Elfeil, Wael K. Youssef, Fatma M. Harb, Sawsan M. Abd-Allah, Ehab M. |
author_facet | Eid, Hamza M. Algammal, Abdelazeem M. Elfeil, Wael K. Youssef, Fatma M. Harb, Sawsan M. Abd-Allah, Ehab M. |
author_sort | Eid, Hamza M. |
collection | PubMed |
description | AIM: This study aimed to investigate the prevalence of different bacterial species affecting ducks as well as demonstrating the antimicrobial susceptibility and molecular typing of the isolated strains. MATERIALS AND METHODS: A total of 500 samples were randomly collected from different duck farms at Ismailia Governorate, Egypt. The collected samples were subjected to the bacteriological examination. Polymerase chain reaction (PCR) was applied for amplification of Kmt1 gene of Pasteurella multocida and X region of protein-A (spA) gene of the isolated Staphylococcus aureus strains to ensure their virulence. The antibiotic sensitivity test was carried out. RESULTS: The most common pathogens isolated from apparently healthy and diseased ducks were P. multocida (10.4% and 25.2%), Escherichia coli (3.6% and 22.8%), Staphylococcus epidermidis (10% and 8.8%), Pseudomonas aeruginosa (2% and 10%), and Proteus vulgaris (0.8% and 10%), respectively. In addition, S. aureus and Salmonella spp. were isolated only from the diseased ducks with prevalence (12.2%) and (2.8%), respectively. Serotyping of the isolated E. coli strains revealed that 25 E. coli strains were belonged to five different serovars O1, O18, O111, O78, and O26, whereas three strains were untypable. Salmonella serotyping showed that all the isolated strains were Salmonella Typhimurium. PCR revealed that four tested P. multocida strains were positive for Kmt1 gene with specific amplicon size 460 bp, while three strains were negative. In addition, all the tested S. aureus strains were positive for spA gene with specific amplicon size 226 bp. The antibiotic sensitivity test revealed that most of the isolated strains were sensitive to enrofloxacin, norfloxacin, and ciprofloxacin. CONCLUSION: P. multocida is the most predominant microorganism isolated from apparently healthy and diseased ducks followed by E. coli and Staphylococci. The combination of both phenotypic and genotypic characterization is more reliable an epidemiological tool for identification of bacterial pathogens affecting ducks. |
format | Online Article Text |
id | pubmed-6584852 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-65848522019-07-19 Prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks Eid, Hamza M. Algammal, Abdelazeem M. Elfeil, Wael K. Youssef, Fatma M. Harb, Sawsan M. Abd-Allah, Ehab M. Vet World Research Article AIM: This study aimed to investigate the prevalence of different bacterial species affecting ducks as well as demonstrating the antimicrobial susceptibility and molecular typing of the isolated strains. MATERIALS AND METHODS: A total of 500 samples were randomly collected from different duck farms at Ismailia Governorate, Egypt. The collected samples were subjected to the bacteriological examination. Polymerase chain reaction (PCR) was applied for amplification of Kmt1 gene of Pasteurella multocida and X region of protein-A (spA) gene of the isolated Staphylococcus aureus strains to ensure their virulence. The antibiotic sensitivity test was carried out. RESULTS: The most common pathogens isolated from apparently healthy and diseased ducks were P. multocida (10.4% and 25.2%), Escherichia coli (3.6% and 22.8%), Staphylococcus epidermidis (10% and 8.8%), Pseudomonas aeruginosa (2% and 10%), and Proteus vulgaris (0.8% and 10%), respectively. In addition, S. aureus and Salmonella spp. were isolated only from the diseased ducks with prevalence (12.2%) and (2.8%), respectively. Serotyping of the isolated E. coli strains revealed that 25 E. coli strains were belonged to five different serovars O1, O18, O111, O78, and O26, whereas three strains were untypable. Salmonella serotyping showed that all the isolated strains were Salmonella Typhimurium. PCR revealed that four tested P. multocida strains were positive for Kmt1 gene with specific amplicon size 460 bp, while three strains were negative. In addition, all the tested S. aureus strains were positive for spA gene with specific amplicon size 226 bp. The antibiotic sensitivity test revealed that most of the isolated strains were sensitive to enrofloxacin, norfloxacin, and ciprofloxacin. CONCLUSION: P. multocida is the most predominant microorganism isolated from apparently healthy and diseased ducks followed by E. coli and Staphylococci. The combination of both phenotypic and genotypic characterization is more reliable an epidemiological tool for identification of bacterial pathogens affecting ducks. Veterinary World 2019-05 2019-05-18 /pmc/articles/PMC6584852/ /pubmed/31327903 http://dx.doi.org/10.14202/vetworld.2019.677-683 Text en Copyright: © Eid, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Eid, Hamza M. Algammal, Abdelazeem M. Elfeil, Wael K. Youssef, Fatma M. Harb, Sawsan M. Abd-Allah, Ehab M. Prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks |
title | Prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks |
title_full | Prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks |
title_fullStr | Prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks |
title_full_unstemmed | Prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks |
title_short | Prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks |
title_sort | prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584852/ https://www.ncbi.nlm.nih.gov/pubmed/31327903 http://dx.doi.org/10.14202/vetworld.2019.677-683 |
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