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Dynamic DNA methylation of ovaries during pubertal transition in gilts
BACKGROUND: In female mammals, the initiation of puberty, coupling with the dramatically morphological changes in ovaries, indicates the sexual and follicular maturation. Previous studies have suggested that the disrupted DNA methylation results in the delayed puberty. However, to date, the changes...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6585006/ https://www.ncbi.nlm.nih.gov/pubmed/31221102 http://dx.doi.org/10.1186/s12864-019-5884-x |
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author | Yuan, Xiaolong Ye, Shaopan Chen, Zitao Pan, Xiangchun Huang, Shuwen Li, Zhonghui Zhong, Yuyi Gao, Ning Zhang, Hao Li, Jiaqi Zhang, Zhe |
author_facet | Yuan, Xiaolong Ye, Shaopan Chen, Zitao Pan, Xiangchun Huang, Shuwen Li, Zhonghui Zhong, Yuyi Gao, Ning Zhang, Hao Li, Jiaqi Zhang, Zhe |
author_sort | Yuan, Xiaolong |
collection | PubMed |
description | BACKGROUND: In female mammals, the initiation of puberty, coupling with the dramatically morphological changes in ovaries, indicates the sexual and follicular maturation. Previous studies have suggested that the disrupted DNA methylation results in the delayed puberty. However, to date, the changes in ovarian methylomes during pubertal transition have not been investigated. In this study, using gilts as a pubertal model, the genome-wide DNA methylation were profiled to explore their dynamics during pubertal transition across Pre-, In- and Post-puberty. RESULTS: During pubertal transition, the follicles underwent maturation and luteinization, coupled with the significant changes in the mRNA expression of DNMT1 and DNMT3a. DNA methylation levels of In-puberty were higher than that of Pre- and Post-puberty at the locations of genes and CpG islands (CGIs). Analysis of the DNA methylation changes identified 12,313, 20,960 and 17,694 differentially methylated CpGs (DMCs) for the comparisons of Pre- vs. In-, In vs. Post-, and Pre- vs. Post-puberty, respectively. Moreover, the CGIs, upstream and exonic regions showed a significant underrepresentation of DMCs, but the CGI shores, CGI shelves, intronic, downstream and intergenic regions showed a significant overrepresentation of DMCs. Furthermore, biological functions of these methylation changes enriched in PI3K-Akt signaling pathway, GnRH signaling pathway, and Insulin secretion, and the mRNA expressions of several genes of these signaling pathway, including MMP2, ESR1, GSK3B, FGF21, IGF1R, and TAC3, were significantly changed across Pre-, In- and Post-puberty in ovaries. CONCLUSIONS: During pubertal transition in gilts, the DNA methylation changes of ovaries were likely to affect the transcription of genes related to PI3K-Akt signaling pathway, GnRH signaling pathway, and Insulin secretion. These observations can provide new insight into the epigenetic mechanism of follicular and sexual maturation during pubertal transition in mammals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5884-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6585006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-65850062019-06-27 Dynamic DNA methylation of ovaries during pubertal transition in gilts Yuan, Xiaolong Ye, Shaopan Chen, Zitao Pan, Xiangchun Huang, Shuwen Li, Zhonghui Zhong, Yuyi Gao, Ning Zhang, Hao Li, Jiaqi Zhang, Zhe BMC Genomics Research Article BACKGROUND: In female mammals, the initiation of puberty, coupling with the dramatically morphological changes in ovaries, indicates the sexual and follicular maturation. Previous studies have suggested that the disrupted DNA methylation results in the delayed puberty. However, to date, the changes in ovarian methylomes during pubertal transition have not been investigated. In this study, using gilts as a pubertal model, the genome-wide DNA methylation were profiled to explore their dynamics during pubertal transition across Pre-, In- and Post-puberty. RESULTS: During pubertal transition, the follicles underwent maturation and luteinization, coupled with the significant changes in the mRNA expression of DNMT1 and DNMT3a. DNA methylation levels of In-puberty were higher than that of Pre- and Post-puberty at the locations of genes and CpG islands (CGIs). Analysis of the DNA methylation changes identified 12,313, 20,960 and 17,694 differentially methylated CpGs (DMCs) for the comparisons of Pre- vs. In-, In vs. Post-, and Pre- vs. Post-puberty, respectively. Moreover, the CGIs, upstream and exonic regions showed a significant underrepresentation of DMCs, but the CGI shores, CGI shelves, intronic, downstream and intergenic regions showed a significant overrepresentation of DMCs. Furthermore, biological functions of these methylation changes enriched in PI3K-Akt signaling pathway, GnRH signaling pathway, and Insulin secretion, and the mRNA expressions of several genes of these signaling pathway, including MMP2, ESR1, GSK3B, FGF21, IGF1R, and TAC3, were significantly changed across Pre-, In- and Post-puberty in ovaries. CONCLUSIONS: During pubertal transition in gilts, the DNA methylation changes of ovaries were likely to affect the transcription of genes related to PI3K-Akt signaling pathway, GnRH signaling pathway, and Insulin secretion. These observations can provide new insight into the epigenetic mechanism of follicular and sexual maturation during pubertal transition in mammals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5884-x) contains supplementary material, which is available to authorized users. BioMed Central 2019-06-20 /pmc/articles/PMC6585006/ /pubmed/31221102 http://dx.doi.org/10.1186/s12864-019-5884-x Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Yuan, Xiaolong Ye, Shaopan Chen, Zitao Pan, Xiangchun Huang, Shuwen Li, Zhonghui Zhong, Yuyi Gao, Ning Zhang, Hao Li, Jiaqi Zhang, Zhe Dynamic DNA methylation of ovaries during pubertal transition in gilts |
title | Dynamic DNA methylation of ovaries during pubertal transition in gilts |
title_full | Dynamic DNA methylation of ovaries during pubertal transition in gilts |
title_fullStr | Dynamic DNA methylation of ovaries during pubertal transition in gilts |
title_full_unstemmed | Dynamic DNA methylation of ovaries during pubertal transition in gilts |
title_short | Dynamic DNA methylation of ovaries during pubertal transition in gilts |
title_sort | dynamic dna methylation of ovaries during pubertal transition in gilts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6585006/ https://www.ncbi.nlm.nih.gov/pubmed/31221102 http://dx.doi.org/10.1186/s12864-019-5884-x |
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