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MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection

Zika virus (ZIKV) is an arthropod-borne virus (arbovirus) member of the Flaviviridae family, which has been associated with the development of the congenital Zika syndrome (CZS). RNA viruses, such as flaviviruses, have been reported to exert a profound impact on host microRNAs (miRNAs). Cellular miR...

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Autores principales: Castro, Fernanda L., Geddes, Victor E. V., Monteiro, Fábio L. L., Gonçalves, Raphael M. D. T., Campanati, Loraine, Pezzuto, Paula, Paquin-Proulx, Dominic, Schamber-Reis, Bruno L., Azevedo, Girlene S., Gonçalves, Alessandro L., Cunha, Daniela P., Moreira, Maria Elisabeth L., Vasconcelos, Zilton F. M., Chimeli, Leila, Melo, Adriana, Tanuri, Amilcar, Nixon, Douglas F., Ribeiro-Alves, Marcelo, Aguiar, Renato S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6585135/
https://www.ncbi.nlm.nih.gov/pubmed/31213064
http://dx.doi.org/10.1177/1759091419850983
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author Castro, Fernanda L.
Geddes, Victor E. V.
Monteiro, Fábio L. L.
Gonçalves, Raphael M. D. T.
Campanati, Loraine
Pezzuto, Paula
Paquin-Proulx, Dominic
Schamber-Reis, Bruno L.
Azevedo, Girlene S.
Gonçalves, Alessandro L.
Cunha, Daniela P.
Moreira, Maria Elisabeth L.
Vasconcelos, Zilton F. M.
Chimeli, Leila
Melo, Adriana
Tanuri, Amilcar
Nixon, Douglas F.
Ribeiro-Alves, Marcelo
Aguiar, Renato S.
author_facet Castro, Fernanda L.
Geddes, Victor E. V.
Monteiro, Fábio L. L.
Gonçalves, Raphael M. D. T.
Campanati, Loraine
Pezzuto, Paula
Paquin-Proulx, Dominic
Schamber-Reis, Bruno L.
Azevedo, Girlene S.
Gonçalves, Alessandro L.
Cunha, Daniela P.
Moreira, Maria Elisabeth L.
Vasconcelos, Zilton F. M.
Chimeli, Leila
Melo, Adriana
Tanuri, Amilcar
Nixon, Douglas F.
Ribeiro-Alves, Marcelo
Aguiar, Renato S.
author_sort Castro, Fernanda L.
collection PubMed
description Zika virus (ZIKV) is an arthropod-borne virus (arbovirus) member of the Flaviviridae family, which has been associated with the development of the congenital Zika syndrome (CZS). RNA viruses, such as flaviviruses, have been reported to exert a profound impact on host microRNAs (miRNAs). Cellular miRNAs modulated by ZIKV may help identify cellular pathways of relevance to pathogenesis. Here, we screened 754 human cellular miRNAs modulated by ZIKV infection (Brazilian PE strain) in a neuroblastoma cell line. Seven miRNAs (miR-99a*, miR-126*, miR-190b, miR-361-3p, miR-522-3p, miR-299-5p, and miR-1267) were downregulated during ZIKV infection, while miR-145 was upregulated. Furthermore, 11 miRNAs were exclusively expressed in ZIKV-infected (miR-148a, miR-342-5p, miR-598, and miR-708-3p) or mock cells (miR-208, miR-329, miR-432-5p, miR-488, miR-518b, miR-520g, and miR-767-5p). Furthermore, in silico analysis indicated that some central nervous system, cellular migration, and adhesion function-related biological processes were overrepresented in the list of target genes of the miRNAs regulated in ZIKV-infected cells, especially for miR-145 and miR-148a. The induction of miR-145 and miR-148a was confirmed in postmortem brain samples from stillborn with severe CZS. Finally, we determined the expression regulation of microcephaly related genes through RNA interference pathway caused by ZIKV directly on neuron cells.
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spelling pubmed-65851352019-06-28 MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection Castro, Fernanda L. Geddes, Victor E. V. Monteiro, Fábio L. L. Gonçalves, Raphael M. D. T. Campanati, Loraine Pezzuto, Paula Paquin-Proulx, Dominic Schamber-Reis, Bruno L. Azevedo, Girlene S. Gonçalves, Alessandro L. Cunha, Daniela P. Moreira, Maria Elisabeth L. Vasconcelos, Zilton F. M. Chimeli, Leila Melo, Adriana Tanuri, Amilcar Nixon, Douglas F. Ribeiro-Alves, Marcelo Aguiar, Renato S. ASN Neuro Original Paper Zika virus (ZIKV) is an arthropod-borne virus (arbovirus) member of the Flaviviridae family, which has been associated with the development of the congenital Zika syndrome (CZS). RNA viruses, such as flaviviruses, have been reported to exert a profound impact on host microRNAs (miRNAs). Cellular miRNAs modulated by ZIKV may help identify cellular pathways of relevance to pathogenesis. Here, we screened 754 human cellular miRNAs modulated by ZIKV infection (Brazilian PE strain) in a neuroblastoma cell line. Seven miRNAs (miR-99a*, miR-126*, miR-190b, miR-361-3p, miR-522-3p, miR-299-5p, and miR-1267) were downregulated during ZIKV infection, while miR-145 was upregulated. Furthermore, 11 miRNAs were exclusively expressed in ZIKV-infected (miR-148a, miR-342-5p, miR-598, and miR-708-3p) or mock cells (miR-208, miR-329, miR-432-5p, miR-488, miR-518b, miR-520g, and miR-767-5p). Furthermore, in silico analysis indicated that some central nervous system, cellular migration, and adhesion function-related biological processes were overrepresented in the list of target genes of the miRNAs regulated in ZIKV-infected cells, especially for miR-145 and miR-148a. The induction of miR-145 and miR-148a was confirmed in postmortem brain samples from stillborn with severe CZS. Finally, we determined the expression regulation of microcephaly related genes through RNA interference pathway caused by ZIKV directly on neuron cells. SAGE Publications 2019-06-19 /pmc/articles/PMC6585135/ /pubmed/31213064 http://dx.doi.org/10.1177/1759091419850983 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Paper
Castro, Fernanda L.
Geddes, Victor E. V.
Monteiro, Fábio L. L.
Gonçalves, Raphael M. D. T.
Campanati, Loraine
Pezzuto, Paula
Paquin-Proulx, Dominic
Schamber-Reis, Bruno L.
Azevedo, Girlene S.
Gonçalves, Alessandro L.
Cunha, Daniela P.
Moreira, Maria Elisabeth L.
Vasconcelos, Zilton F. M.
Chimeli, Leila
Melo, Adriana
Tanuri, Amilcar
Nixon, Douglas F.
Ribeiro-Alves, Marcelo
Aguiar, Renato S.
MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection
title MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection
title_full MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection
title_fullStr MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection
title_full_unstemmed MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection
title_short MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection
title_sort micrornas 145 and 148a are upregulated during congenital zika virus infection
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6585135/
https://www.ncbi.nlm.nih.gov/pubmed/31213064
http://dx.doi.org/10.1177/1759091419850983
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