A Versatile and Convenient Synthesis of (34)S‐Labeled Phosphorothioate Oligonucleotides

A synthetic protocol for (34)S‐labeled phosphorothioate oligonucleotides (PS ONs) was developed to facilitate MS‐based assay analysis. This was enabled by a highly efficient, two‐step, one‐pot synthesis of (34)S‐labeled phenylacetyl disulfide ((34)S‐PADS), starting from (34)S‐enriched elemental sulf...

Descripción completa

Detalles Bibliográficos
Autores principales: Stulz, Rouven, Meuller, Johan, Baždarević, Dženita, Wennberg Huldt, Charlotte, Strömberg, Roger, Andersson, Shalini, Dahlén, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6585993/
https://www.ncbi.nlm.nih.gov/pubmed/30062829
http://dx.doi.org/10.1002/cbic.201800417
_version_ 1783428816369090560
author Stulz, Rouven
Meuller, Johan
Baždarević, Dženita
Wennberg Huldt, Charlotte
Strömberg, Roger
Andersson, Shalini
Dahlén, Anders
author_facet Stulz, Rouven
Meuller, Johan
Baždarević, Dženita
Wennberg Huldt, Charlotte
Strömberg, Roger
Andersson, Shalini
Dahlén, Anders
author_sort Stulz, Rouven
collection PubMed
description A synthetic protocol for (34)S‐labeled phosphorothioate oligonucleotides (PS ONs) was developed to facilitate MS‐based assay analysis. This was enabled by a highly efficient, two‐step, one‐pot synthesis of (34)S‐labeled phenylacetyl disulfide ((34)S‐PADS), starting from (34)S‐enriched elemental sulfur ((34)S(8)). (34)S‐PADS was subsequently used for stable isotope labeling (SIL) of oligonucleotides containing a phosphorothioate backbone. The (34)S‐SIL PS ONs are shown to retain the same melting temperature, antisense activity, and secondary structure as those of the corresponding unlabeled (32)S PS ONs.
format Online
Article
Text
id pubmed-6585993
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-65859932019-06-27 A Versatile and Convenient Synthesis of (34)S‐Labeled Phosphorothioate Oligonucleotides Stulz, Rouven Meuller, Johan Baždarević, Dženita Wennberg Huldt, Charlotte Strömberg, Roger Andersson, Shalini Dahlén, Anders Chembiochem Full Papers A synthetic protocol for (34)S‐labeled phosphorothioate oligonucleotides (PS ONs) was developed to facilitate MS‐based assay analysis. This was enabled by a highly efficient, two‐step, one‐pot synthesis of (34)S‐labeled phenylacetyl disulfide ((34)S‐PADS), starting from (34)S‐enriched elemental sulfur ((34)S(8)). (34)S‐PADS was subsequently used for stable isotope labeling (SIL) of oligonucleotides containing a phosphorothioate backbone. The (34)S‐SIL PS ONs are shown to retain the same melting temperature, antisense activity, and secondary structure as those of the corresponding unlabeled (32)S PS ONs. John Wiley and Sons Inc. 2018-09-13 2018-10-04 /pmc/articles/PMC6585993/ /pubmed/30062829 http://dx.doi.org/10.1002/cbic.201800417 Text en © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Full Papers
Stulz, Rouven
Meuller, Johan
Baždarević, Dženita
Wennberg Huldt, Charlotte
Strömberg, Roger
Andersson, Shalini
Dahlén, Anders
A Versatile and Convenient Synthesis of (34)S‐Labeled Phosphorothioate Oligonucleotides
title A Versatile and Convenient Synthesis of (34)S‐Labeled Phosphorothioate Oligonucleotides
title_full A Versatile and Convenient Synthesis of (34)S‐Labeled Phosphorothioate Oligonucleotides
title_fullStr A Versatile and Convenient Synthesis of (34)S‐Labeled Phosphorothioate Oligonucleotides
title_full_unstemmed A Versatile and Convenient Synthesis of (34)S‐Labeled Phosphorothioate Oligonucleotides
title_short A Versatile and Convenient Synthesis of (34)S‐Labeled Phosphorothioate Oligonucleotides
title_sort versatile and convenient synthesis of (34)s‐labeled phosphorothioate oligonucleotides
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6585993/
https://www.ncbi.nlm.nih.gov/pubmed/30062829
http://dx.doi.org/10.1002/cbic.201800417
work_keys_str_mv AT stulzrouven aversatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT meullerjohan aversatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT bazdarevicdzenita aversatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT wennberghuldtcharlotte aversatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT strombergroger aversatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT anderssonshalini aversatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT dahlenanders aversatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT stulzrouven versatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT meullerjohan versatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT bazdarevicdzenita versatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT wennberghuldtcharlotte versatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT strombergroger versatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT anderssonshalini versatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides
AT dahlenanders versatileandconvenientsynthesisof34slabeledphosphorothioateoligonucleotides

Ejemplares similares