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Activity of two hyaluronan preparations on primary human oral fibroblasts

BACKGROUND AND OBJECTIVE: The potential benefit of using hyaluronan (HA) in reconstructive periodontal surgery is still a matter of debate. The aim of the present study was to evaluate the effects of two HA formulations on human oral fibroblasts involved in soft tissue wound healing/regeneration. MA...

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Autores principales: Asparuhova, Maria B., Kiryak, Deniz, Eliezer, Meizi, Mihov, Deyan, Sculean, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586051/
https://www.ncbi.nlm.nih.gov/pubmed/30264516
http://dx.doi.org/10.1111/jre.12602
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author Asparuhova, Maria B.
Kiryak, Deniz
Eliezer, Meizi
Mihov, Deyan
Sculean, Anton
author_facet Asparuhova, Maria B.
Kiryak, Deniz
Eliezer, Meizi
Mihov, Deyan
Sculean, Anton
author_sort Asparuhova, Maria B.
collection PubMed
description BACKGROUND AND OBJECTIVE: The potential benefit of using hyaluronan (HA) in reconstructive periodontal surgery is still a matter of debate. The aim of the present study was to evaluate the effects of two HA formulations on human oral fibroblasts involved in soft tissue wound healing/regeneration. MATERIAL AND METHODS: Metabolic, proliferative and migratory abilities of primary human palatal and gingival fibroblasts were examined upon HA treatment. To uncover the mechanisms whereby HA influences cellular behavior, wound healing‐related gene expression and activation of signaling kinases were analyzed by qRT‐PCR and immunoblotting, respectively. RESULTS: The investigated HA formulations maintained the viability of oral fibroblasts and increased their proliferative and migratory abilities. They enhanced expression of genes encoding type III collagen and transforming growth factor‐β3, characteristic of scarless wound healing. The HAs upregulated the expression of genes encoding pro‐proliferative, pro‐migratory, and pro‐inflammatory factors, with only a moderate effect on the latter in gingival fibroblasts. In palatal but not gingival fibroblasts, an indirect effect of HA on the expression of matrix metalloproteinases 2 and 3 was detected, potentially exerted through induction of pro‐inflammatory cytokines. Finally, our data pointed on Akt, Erk1/2 and p38 as the signaling molecules whereby the HAs exert their effects on oral fibroblasts. CONCLUSION: Both investigated HA formulations are biocompatible and enhance the proliferative, migratory and wound healing properties of cell types involved in soft tissue wound healing following regenerative periodontal surgery. Our data further suggest that in gingival tissues, the HAs are not likely to impair the healing process by prolonging inflammation or causing excessive MMP expression at the repair site.
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spelling pubmed-65860512019-06-27 Activity of two hyaluronan preparations on primary human oral fibroblasts Asparuhova, Maria B. Kiryak, Deniz Eliezer, Meizi Mihov, Deyan Sculean, Anton J Periodontal Res Original Articles BACKGROUND AND OBJECTIVE: The potential benefit of using hyaluronan (HA) in reconstructive periodontal surgery is still a matter of debate. The aim of the present study was to evaluate the effects of two HA formulations on human oral fibroblasts involved in soft tissue wound healing/regeneration. MATERIAL AND METHODS: Metabolic, proliferative and migratory abilities of primary human palatal and gingival fibroblasts were examined upon HA treatment. To uncover the mechanisms whereby HA influences cellular behavior, wound healing‐related gene expression and activation of signaling kinases were analyzed by qRT‐PCR and immunoblotting, respectively. RESULTS: The investigated HA formulations maintained the viability of oral fibroblasts and increased their proliferative and migratory abilities. They enhanced expression of genes encoding type III collagen and transforming growth factor‐β3, characteristic of scarless wound healing. The HAs upregulated the expression of genes encoding pro‐proliferative, pro‐migratory, and pro‐inflammatory factors, with only a moderate effect on the latter in gingival fibroblasts. In palatal but not gingival fibroblasts, an indirect effect of HA on the expression of matrix metalloproteinases 2 and 3 was detected, potentially exerted through induction of pro‐inflammatory cytokines. Finally, our data pointed on Akt, Erk1/2 and p38 as the signaling molecules whereby the HAs exert their effects on oral fibroblasts. CONCLUSION: Both investigated HA formulations are biocompatible and enhance the proliferative, migratory and wound healing properties of cell types involved in soft tissue wound healing following regenerative periodontal surgery. Our data further suggest that in gingival tissues, the HAs are not likely to impair the healing process by prolonging inflammation or causing excessive MMP expression at the repair site. John Wiley and Sons Inc. 2018-09-27 2019-02 /pmc/articles/PMC6586051/ /pubmed/30264516 http://dx.doi.org/10.1111/jre.12602 Text en © 2018 The Authors. Journal of Periodontal Research Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Asparuhova, Maria B.
Kiryak, Deniz
Eliezer, Meizi
Mihov, Deyan
Sculean, Anton
Activity of two hyaluronan preparations on primary human oral fibroblasts
title Activity of two hyaluronan preparations on primary human oral fibroblasts
title_full Activity of two hyaluronan preparations on primary human oral fibroblasts
title_fullStr Activity of two hyaluronan preparations on primary human oral fibroblasts
title_full_unstemmed Activity of two hyaluronan preparations on primary human oral fibroblasts
title_short Activity of two hyaluronan preparations on primary human oral fibroblasts
title_sort activity of two hyaluronan preparations on primary human oral fibroblasts
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586051/
https://www.ncbi.nlm.nih.gov/pubmed/30264516
http://dx.doi.org/10.1111/jre.12602
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