Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis

In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed i...

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Autores principales: Kim, Juyun, Kim, Namgyu, Mannaa, Mohamed, Lee, Hyun-Hee, Jeon, Jong-Seong, Seo, Young-Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2019
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586193/
https://www.ncbi.nlm.nih.gov/pubmed/31244573
http://dx.doi.org/10.5423/PPJ.NT.02.2019.0026
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author Kim, Juyun
Kim, Namgyu
Mannaa, Mohamed
Lee, Hyun-Hee
Jeon, Jong-Seong
Seo, Young-Su
author_facet Kim, Juyun
Kim, Namgyu
Mannaa, Mohamed
Lee, Hyun-Hee
Jeon, Jong-Seong
Seo, Young-Su
author_sort Kim, Juyun
collection PubMed
description In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae-infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent.
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spelling pubmed-65861932019-06-26 Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis Kim, Juyun Kim, Namgyu Mannaa, Mohamed Lee, Hyun-Hee Jeon, Jong-Seong Seo, Young-Su Plant Pathol J Note In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae-infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent. Korean Society of Plant Pathology 2019-06 2019-06-01 /pmc/articles/PMC6586193/ /pubmed/31244573 http://dx.doi.org/10.5423/PPJ.NT.02.2019.0026 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Kim, Juyun
Kim, Namgyu
Mannaa, Mohamed
Lee, Hyun-Hee
Jeon, Jong-Seong
Seo, Young-Su
Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis
title Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis
title_full Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis
title_fullStr Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis
title_full_unstemmed Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis
title_short Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis
title_sort characterization of burkholderia glumae putative virulence factor 11 (pvf11) via yeast two-hybrid interaction and phenotypic analysis
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586193/
https://www.ncbi.nlm.nih.gov/pubmed/31244573
http://dx.doi.org/10.5423/PPJ.NT.02.2019.0026
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