Cargando…

Prevalence and cervical organism burden among Louisiana women with Trichomonas vaginalis infections

Trichomonas vaginalis is the most common curable sexually transmitted infection (STI) worldwide. Although predominately asymptomatic, the disease spectrum of trichomoniasis in women is characterized primarily by signs and symptoms of vaginitis, including purulent discharge and localized vulvar pruri...

Descripción completa

Detalles Bibliográficos
Autores principales: Shaw, Meredith K., Porterfield, Harry S., Favaloro, Sue, Dehon, Patricia M., Van Der Pol, Barbara, Quayle, Alison J., McGowin, Chris L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586396/
https://www.ncbi.nlm.nih.gov/pubmed/31220094
http://dx.doi.org/10.1371/journal.pone.0217041
_version_ 1783428888579276800
author Shaw, Meredith K.
Porterfield, Harry S.
Favaloro, Sue
Dehon, Patricia M.
Van Der Pol, Barbara
Quayle, Alison J.
McGowin, Chris L.
author_facet Shaw, Meredith K.
Porterfield, Harry S.
Favaloro, Sue
Dehon, Patricia M.
Van Der Pol, Barbara
Quayle, Alison J.
McGowin, Chris L.
author_sort Shaw, Meredith K.
collection PubMed
description Trichomonas vaginalis is the most common curable sexually transmitted infection (STI) worldwide. Although predominately asymptomatic, the disease spectrum of trichomoniasis in women is characterized primarily by signs and symptoms of vaginitis, including purulent discharge and localized vulvar pruritus and erythema. Several FDA-cleared nucleic acid amplification tests (NAATs) are available for the diagnosis of T. vaginalis infections, but laboratory developed tests (LDTs) are widely utilized and cost-effective solutions in both the research and clinical diagnostic settings. LDT diagnosis of T. vaginalis is particularly appealing since it can be performed using remnant specimens collected for other STI testing. Using a LDT implemented as part of this study, T. vaginalis was detected in 7% of participating Louisiana women (14/199). The mean T. vaginalis organism burden was 1.0x10(6) ± 4.5x10(5) organisms per mL of ThinPrep PreservCyt. Using DNA eluates obtained after HPV testing on the cobas 4800 system, the T. vaginalis LDT was characterized by excellent intra- and interassay reproducibility (coefficient of variation values all <3.5%). Compared with two commercially available NAATs from TIB MOLBIOL, the sensitivity and specificity of the LDT was 92.9 and 99.5%, respectively. Collectively, this study details the diagnostic and quantitative utility of a LDT for T. vaginalis. When applied in the clinical research setting, we confirmed the high prevalence of T. vaginalis, but also observed extraordinarily high organism burdens in the cervix. These findings highlight the unique host-pathogen relationship of T. vaginalis with lower reproductive tract tissues, and substantiate the need for continued investigation of this highly prevalent STI.
format Online
Article
Text
id pubmed-6586396
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-65863962019-06-28 Prevalence and cervical organism burden among Louisiana women with Trichomonas vaginalis infections Shaw, Meredith K. Porterfield, Harry S. Favaloro, Sue Dehon, Patricia M. Van Der Pol, Barbara Quayle, Alison J. McGowin, Chris L. PLoS One Research Article Trichomonas vaginalis is the most common curable sexually transmitted infection (STI) worldwide. Although predominately asymptomatic, the disease spectrum of trichomoniasis in women is characterized primarily by signs and symptoms of vaginitis, including purulent discharge and localized vulvar pruritus and erythema. Several FDA-cleared nucleic acid amplification tests (NAATs) are available for the diagnosis of T. vaginalis infections, but laboratory developed tests (LDTs) are widely utilized and cost-effective solutions in both the research and clinical diagnostic settings. LDT diagnosis of T. vaginalis is particularly appealing since it can be performed using remnant specimens collected for other STI testing. Using a LDT implemented as part of this study, T. vaginalis was detected in 7% of participating Louisiana women (14/199). The mean T. vaginalis organism burden was 1.0x10(6) ± 4.5x10(5) organisms per mL of ThinPrep PreservCyt. Using DNA eluates obtained after HPV testing on the cobas 4800 system, the T. vaginalis LDT was characterized by excellent intra- and interassay reproducibility (coefficient of variation values all <3.5%). Compared with two commercially available NAATs from TIB MOLBIOL, the sensitivity and specificity of the LDT was 92.9 and 99.5%, respectively. Collectively, this study details the diagnostic and quantitative utility of a LDT for T. vaginalis. When applied in the clinical research setting, we confirmed the high prevalence of T. vaginalis, but also observed extraordinarily high organism burdens in the cervix. These findings highlight the unique host-pathogen relationship of T. vaginalis with lower reproductive tract tissues, and substantiate the need for continued investigation of this highly prevalent STI. Public Library of Science 2019-06-20 /pmc/articles/PMC6586396/ /pubmed/31220094 http://dx.doi.org/10.1371/journal.pone.0217041 Text en © 2019 Shaw et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shaw, Meredith K.
Porterfield, Harry S.
Favaloro, Sue
Dehon, Patricia M.
Van Der Pol, Barbara
Quayle, Alison J.
McGowin, Chris L.
Prevalence and cervical organism burden among Louisiana women with Trichomonas vaginalis infections
title Prevalence and cervical organism burden among Louisiana women with Trichomonas vaginalis infections
title_full Prevalence and cervical organism burden among Louisiana women with Trichomonas vaginalis infections
title_fullStr Prevalence and cervical organism burden among Louisiana women with Trichomonas vaginalis infections
title_full_unstemmed Prevalence and cervical organism burden among Louisiana women with Trichomonas vaginalis infections
title_short Prevalence and cervical organism burden among Louisiana women with Trichomonas vaginalis infections
title_sort prevalence and cervical organism burden among louisiana women with trichomonas vaginalis infections
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586396/
https://www.ncbi.nlm.nih.gov/pubmed/31220094
http://dx.doi.org/10.1371/journal.pone.0217041
work_keys_str_mv AT shawmeredithk prevalenceandcervicalorganismburdenamonglouisianawomenwithtrichomonasvaginalisinfections
AT porterfieldharrys prevalenceandcervicalorganismburdenamonglouisianawomenwithtrichomonasvaginalisinfections
AT favalorosue prevalenceandcervicalorganismburdenamonglouisianawomenwithtrichomonasvaginalisinfections
AT dehonpatriciam prevalenceandcervicalorganismburdenamonglouisianawomenwithtrichomonasvaginalisinfections
AT vanderpolbarbara prevalenceandcervicalorganismburdenamonglouisianawomenwithtrichomonasvaginalisinfections
AT quaylealisonj prevalenceandcervicalorganismburdenamonglouisianawomenwithtrichomonasvaginalisinfections
AT mcgowinchrisl prevalenceandcervicalorganismburdenamonglouisianawomenwithtrichomonasvaginalisinfections