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Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases

Traumatic spinal cord injury has dramatic consequences and a huge social impact. We propose a new mouse model of spinal trauma that induces a complete paralysis of hindlimbs, still observable 30 days after injury. The contusion, performed without laminectomy and deriving from the pressure exerted di...

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Autores principales: Marinelli, Sara, Vacca, Valentina, De Angelis, Federica, Pieroni, Luisa, Orsini, Tiziana, Parisi, Chiara, Soligo, Marzia, Protto, Virginia, Manni, Luigi, Guerrieri, Roberto, Pavone, Flaminia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586623/
https://www.ncbi.nlm.nih.gov/pubmed/31222077
http://dx.doi.org/10.1038/s41598-019-45037-x
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author Marinelli, Sara
Vacca, Valentina
De Angelis, Federica
Pieroni, Luisa
Orsini, Tiziana
Parisi, Chiara
Soligo, Marzia
Protto, Virginia
Manni, Luigi
Guerrieri, Roberto
Pavone, Flaminia
author_facet Marinelli, Sara
Vacca, Valentina
De Angelis, Federica
Pieroni, Luisa
Orsini, Tiziana
Parisi, Chiara
Soligo, Marzia
Protto, Virginia
Manni, Luigi
Guerrieri, Roberto
Pavone, Flaminia
author_sort Marinelli, Sara
collection PubMed
description Traumatic spinal cord injury has dramatic consequences and a huge social impact. We propose a new mouse model of spinal trauma that induces a complete paralysis of hindlimbs, still observable 30 days after injury. The contusion, performed without laminectomy and deriving from the pressure exerted directly on the bone, mimics more closely many features of spinal injury in humans. Spinal cord was injured at thoracic level 10 (T10) in adult anesthetized female CD1 mice, mounted on stereotaxic apparatus and connected to a precision impactor device. Following severe injury, we evaluated motor and sensory functions, and histological/morphological features of spinal tissue at different time points. Moreover, we studied the effects of early and subchronic administration of Docosahexaenoic acid, investigating functional responses, structural changes proximal and distal to the lesion in primary and secondary injury phases, proteome modulation in injured spinal cord. Docosahexaenoic acid was able i) to restore behavioural responses and ii) to induce pro-regenerative effects and neuroprotective action against demyelination, apoptosis and neuroinflammation. Considering the urgent health challenge represented by spinal injury, this new and reliable mouse model together with the positive effects of docosahexaenoic acid provide important translational implications for promising therapeutic approaches for spinal cord injuries.
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spelling pubmed-65866232019-06-26 Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases Marinelli, Sara Vacca, Valentina De Angelis, Federica Pieroni, Luisa Orsini, Tiziana Parisi, Chiara Soligo, Marzia Protto, Virginia Manni, Luigi Guerrieri, Roberto Pavone, Flaminia Sci Rep Article Traumatic spinal cord injury has dramatic consequences and a huge social impact. We propose a new mouse model of spinal trauma that induces a complete paralysis of hindlimbs, still observable 30 days after injury. The contusion, performed without laminectomy and deriving from the pressure exerted directly on the bone, mimics more closely many features of spinal injury in humans. Spinal cord was injured at thoracic level 10 (T10) in adult anesthetized female CD1 mice, mounted on stereotaxic apparatus and connected to a precision impactor device. Following severe injury, we evaluated motor and sensory functions, and histological/morphological features of spinal tissue at different time points. Moreover, we studied the effects of early and subchronic administration of Docosahexaenoic acid, investigating functional responses, structural changes proximal and distal to the lesion in primary and secondary injury phases, proteome modulation in injured spinal cord. Docosahexaenoic acid was able i) to restore behavioural responses and ii) to induce pro-regenerative effects and neuroprotective action against demyelination, apoptosis and neuroinflammation. Considering the urgent health challenge represented by spinal injury, this new and reliable mouse model together with the positive effects of docosahexaenoic acid provide important translational implications for promising therapeutic approaches for spinal cord injuries. Nature Publishing Group UK 2019-06-20 /pmc/articles/PMC6586623/ /pubmed/31222077 http://dx.doi.org/10.1038/s41598-019-45037-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Marinelli, Sara
Vacca, Valentina
De Angelis, Federica
Pieroni, Luisa
Orsini, Tiziana
Parisi, Chiara
Soligo, Marzia
Protto, Virginia
Manni, Luigi
Guerrieri, Roberto
Pavone, Flaminia
Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases
title Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases
title_full Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases
title_fullStr Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases
title_full_unstemmed Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases
title_short Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases
title_sort innovative mouse model mimicking human-like features of spinal cord injury: efficacy of docosahexaenoic acid on acute and chronic phases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586623/
https://www.ncbi.nlm.nih.gov/pubmed/31222077
http://dx.doi.org/10.1038/s41598-019-45037-x
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