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Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen

Enterococci are subdominant members of the human gastrointestinal microbiota. Enterococcus faecalis is generally harmless for healthy individuals, but it can cause a diverse range of infections in immunodeficient or elderly patients with severe underlying diseases. In this study, we analysed the lev...

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Autores principales: Archambaud, Cristel, Derré-Bobillot, Aurélie, Lapaque, Nicolas, Rigottier-Gois, Lionel, Serror, Pascale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586816/
https://www.ncbi.nlm.nih.gov/pubmed/31222056
http://dx.doi.org/10.1038/s41598-019-45441-3
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author Archambaud, Cristel
Derré-Bobillot, Aurélie
Lapaque, Nicolas
Rigottier-Gois, Lionel
Serror, Pascale
author_facet Archambaud, Cristel
Derré-Bobillot, Aurélie
Lapaque, Nicolas
Rigottier-Gois, Lionel
Serror, Pascale
author_sort Archambaud, Cristel
collection PubMed
description Enterococci are subdominant members of the human gastrointestinal microbiota. Enterococcus faecalis is generally harmless for healthy individuals, but it can cause a diverse range of infections in immunodeficient or elderly patients with severe underlying diseases. In this study, we analysed the levels of intestinal translocation of indigenous enterococci in C57BL/6, CF-1 and CX3CR1(−/−) mice upon clindamycin antibiotic-induced dysbiosis. We found that C57BL/6 was the most permissive model for enterococcal translocation and that initiation of E. faecalis translocation coincided with a threshold of enterococcal colonisation in the gut lumen, which once reached, triggered E. faecalis dissemination to deeper organs. We showed that the extent to which E. faecalis clinical strain VE14821 competed with indigenous enterococci differed between the C57BL/6 and CX3CR1(−/−) models. Finally, using a simplified gnotobiotic model, we observed E. faecalis crossing an intact intestinal tract using intestinal epithelial cells as one route to reach the lamina propria. Our study opens new perspectives for assessing the effect of various immunodeficiencies and for investigating mechanisms underlying enterococcal translocation.
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spelling pubmed-65868162019-06-27 Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen Archambaud, Cristel Derré-Bobillot, Aurélie Lapaque, Nicolas Rigottier-Gois, Lionel Serror, Pascale Sci Rep Article Enterococci are subdominant members of the human gastrointestinal microbiota. Enterococcus faecalis is generally harmless for healthy individuals, but it can cause a diverse range of infections in immunodeficient or elderly patients with severe underlying diseases. In this study, we analysed the levels of intestinal translocation of indigenous enterococci in C57BL/6, CF-1 and CX3CR1(−/−) mice upon clindamycin antibiotic-induced dysbiosis. We found that C57BL/6 was the most permissive model for enterococcal translocation and that initiation of E. faecalis translocation coincided with a threshold of enterococcal colonisation in the gut lumen, which once reached, triggered E. faecalis dissemination to deeper organs. We showed that the extent to which E. faecalis clinical strain VE14821 competed with indigenous enterococci differed between the C57BL/6 and CX3CR1(−/−) models. Finally, using a simplified gnotobiotic model, we observed E. faecalis crossing an intact intestinal tract using intestinal epithelial cells as one route to reach the lamina propria. Our study opens new perspectives for assessing the effect of various immunodeficiencies and for investigating mechanisms underlying enterococcal translocation. Nature Publishing Group UK 2019-06-20 /pmc/articles/PMC6586816/ /pubmed/31222056 http://dx.doi.org/10.1038/s41598-019-45441-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Archambaud, Cristel
Derré-Bobillot, Aurélie
Lapaque, Nicolas
Rigottier-Gois, Lionel
Serror, Pascale
Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen
title Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen
title_full Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen
title_fullStr Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen
title_full_unstemmed Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen
title_short Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen
title_sort intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586816/
https://www.ncbi.nlm.nih.gov/pubmed/31222056
http://dx.doi.org/10.1038/s41598-019-45441-3
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