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Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes

We analyzed the associations between whole blood microRNA profiles and the indices of glucose metabolism and impaired fasting glucose and examined whether the discovered microRNAs correlate with the expression of their mRNA targets. MicroRNA and gene expression profiling were performed for the Young...

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Autores principales: Mononen, Nina, Lyytikäinen, Leo-Pekka, Seppälä, Ilkka, Mishra, Pashupati P., Juonala, Markus, Waldenberger, Melanie, Klopp, Norman, Illig, Thomas, Leiviskä, Jaana, Loo, Britt-Marie, Laaksonen, Reijo, Oksala, Niku, Kähönen, Mika, Hutri-Kähönen, Nina, Raitakari, Olli, Lehtimäki, Terho, Raitoharju, Emma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586838/
https://www.ncbi.nlm.nih.gov/pubmed/31222113
http://dx.doi.org/10.1038/s41598-019-43793-4
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author Mononen, Nina
Lyytikäinen, Leo-Pekka
Seppälä, Ilkka
Mishra, Pashupati P.
Juonala, Markus
Waldenberger, Melanie
Klopp, Norman
Illig, Thomas
Leiviskä, Jaana
Loo, Britt-Marie
Laaksonen, Reijo
Oksala, Niku
Kähönen, Mika
Hutri-Kähönen, Nina
Raitakari, Olli
Lehtimäki, Terho
Raitoharju, Emma
author_facet Mononen, Nina
Lyytikäinen, Leo-Pekka
Seppälä, Ilkka
Mishra, Pashupati P.
Juonala, Markus
Waldenberger, Melanie
Klopp, Norman
Illig, Thomas
Leiviskä, Jaana
Loo, Britt-Marie
Laaksonen, Reijo
Oksala, Niku
Kähönen, Mika
Hutri-Kähönen, Nina
Raitakari, Olli
Lehtimäki, Terho
Raitoharju, Emma
author_sort Mononen, Nina
collection PubMed
description We analyzed the associations between whole blood microRNA profiles and the indices of glucose metabolism and impaired fasting glucose and examined whether the discovered microRNAs correlate with the expression of their mRNA targets. MicroRNA and gene expression profiling were performed for the Young Finns Study participants (n = 871). Glucose, insulin, and glycated hemoglobin (HbA1c) levels were measured, the insulin resistance index (HOMA2-IR) was calculated, and the glycemic status (normoglycemic [n = 534]/impaired fasting glucose [IFG] [n = 252]/type 2 diabetes [T2D] [n = 24]) determined. Levels of hsa-miR-144-5p, -122-5p, -148a-3p, -589-5p, and hsa-let-7a-5p associated with glycemic status. hsa-miR-144-5p and -148a-3p associated with glucose levels, while hsa-miR-144-5p, -122-5p, -184, and -339-3p associated with insulin levels and HOMA2-IR, and hsa-miR-148a-3p, -15b-3p, -93-3p, -146b-5p, -221-3p, -18a-3p, -642a-5p, and -181-2-3p associated with HbA1c levels. The targets of hsa-miR-146b-5p that correlated with its levels were enriched in inflammatory pathways, and the targets of hsa-miR-221-3p were enriched in insulin signaling and T2D pathways. These pathways showed indications of co-regulation by HbA1c-associated miRNAs. There were significant differences in the microRNA profiles associated with glucose, insulin, or HOMA-IR compared to those associated with HbA1c. The HbA1c-associated miRNAs also correlated with the expression of target mRNAs in pathways important to the development of T2D.
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spelling pubmed-65868382019-06-27 Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes Mononen, Nina Lyytikäinen, Leo-Pekka Seppälä, Ilkka Mishra, Pashupati P. Juonala, Markus Waldenberger, Melanie Klopp, Norman Illig, Thomas Leiviskä, Jaana Loo, Britt-Marie Laaksonen, Reijo Oksala, Niku Kähönen, Mika Hutri-Kähönen, Nina Raitakari, Olli Lehtimäki, Terho Raitoharju, Emma Sci Rep Article We analyzed the associations between whole blood microRNA profiles and the indices of glucose metabolism and impaired fasting glucose and examined whether the discovered microRNAs correlate with the expression of their mRNA targets. MicroRNA and gene expression profiling were performed for the Young Finns Study participants (n = 871). Glucose, insulin, and glycated hemoglobin (HbA1c) levels were measured, the insulin resistance index (HOMA2-IR) was calculated, and the glycemic status (normoglycemic [n = 534]/impaired fasting glucose [IFG] [n = 252]/type 2 diabetes [T2D] [n = 24]) determined. Levels of hsa-miR-144-5p, -122-5p, -148a-3p, -589-5p, and hsa-let-7a-5p associated with glycemic status. hsa-miR-144-5p and -148a-3p associated with glucose levels, while hsa-miR-144-5p, -122-5p, -184, and -339-3p associated with insulin levels and HOMA2-IR, and hsa-miR-148a-3p, -15b-3p, -93-3p, -146b-5p, -221-3p, -18a-3p, -642a-5p, and -181-2-3p associated with HbA1c levels. The targets of hsa-miR-146b-5p that correlated with its levels were enriched in inflammatory pathways, and the targets of hsa-miR-221-3p were enriched in insulin signaling and T2D pathways. These pathways showed indications of co-regulation by HbA1c-associated miRNAs. There were significant differences in the microRNA profiles associated with glucose, insulin, or HOMA-IR compared to those associated with HbA1c. The HbA1c-associated miRNAs also correlated with the expression of target mRNAs in pathways important to the development of T2D. Nature Publishing Group UK 2019-06-20 /pmc/articles/PMC6586838/ /pubmed/31222113 http://dx.doi.org/10.1038/s41598-019-43793-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mononen, Nina
Lyytikäinen, Leo-Pekka
Seppälä, Ilkka
Mishra, Pashupati P.
Juonala, Markus
Waldenberger, Melanie
Klopp, Norman
Illig, Thomas
Leiviskä, Jaana
Loo, Britt-Marie
Laaksonen, Reijo
Oksala, Niku
Kähönen, Mika
Hutri-Kähönen, Nina
Raitakari, Olli
Lehtimäki, Terho
Raitoharju, Emma
Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes
title Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes
title_full Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes
title_fullStr Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes
title_full_unstemmed Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes
title_short Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes
title_sort whole blood microrna levels associate with glycemic status and correlate with target mrnas in pathways important to type 2 diabetes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586838/
https://www.ncbi.nlm.nih.gov/pubmed/31222113
http://dx.doi.org/10.1038/s41598-019-43793-4
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