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Characterization of C-nucleoside Antimicrobials from Streptomyces albus DSM 40763: Strepturidin is Pseudouridimycin
Pseudouridimycin (PUM), a selective inhibitor of bacterial RNA polymerase has been previously detected in microbial-extracts of two strains of Streptomyces species (strain ID38640 and ID38673). Here, we isolated PUM and its deoxygenated analogue desoxy-pseudouridimycin (dPUM) from Streptomyces albus...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586884/ https://www.ncbi.nlm.nih.gov/pubmed/31222036 http://dx.doi.org/10.1038/s41598-019-45375-w |
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author | Rosenqvist, Petja Palmu, Kaisa Prajapati, Ranjit Kumar Yamada, Keith Niemi, Jarmo Belogurov, Georgiy A. Metsä-Ketelä, Mikko Virta, Pasi |
author_facet | Rosenqvist, Petja Palmu, Kaisa Prajapati, Ranjit Kumar Yamada, Keith Niemi, Jarmo Belogurov, Georgiy A. Metsä-Ketelä, Mikko Virta, Pasi |
author_sort | Rosenqvist, Petja |
collection | PubMed |
description | Pseudouridimycin (PUM), a selective inhibitor of bacterial RNA polymerase has been previously detected in microbial-extracts of two strains of Streptomyces species (strain ID38640 and ID38673). Here, we isolated PUM and its deoxygenated analogue desoxy-pseudouridimycin (dPUM) from Streptomyces albus DSM 40763, previously reported to produce the metabolite strepturidin (STU). The isolated compounds were characterized by HRMS and spectroscopic techniques and they selectively inhibited transcription by bacterial RNA polymerase as previously reported for PUM. In contrast, STU could not be detected in the cultures of S. albus DSM 40763. As the reported characteristics reported for STU are almost identical with that of PUM, the existence of STU was questioned. We further sequenced the genome of S. albus DSM 40763 and identified a gene cluster that contains orthologs of all PUM biosynthesis enzymes but lacks the enzymes that would conceivably allow biosynthesis of STU as an additional product. |
format | Online Article Text |
id | pubmed-6586884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65868842019-06-27 Characterization of C-nucleoside Antimicrobials from Streptomyces albus DSM 40763: Strepturidin is Pseudouridimycin Rosenqvist, Petja Palmu, Kaisa Prajapati, Ranjit Kumar Yamada, Keith Niemi, Jarmo Belogurov, Georgiy A. Metsä-Ketelä, Mikko Virta, Pasi Sci Rep Article Pseudouridimycin (PUM), a selective inhibitor of bacterial RNA polymerase has been previously detected in microbial-extracts of two strains of Streptomyces species (strain ID38640 and ID38673). Here, we isolated PUM and its deoxygenated analogue desoxy-pseudouridimycin (dPUM) from Streptomyces albus DSM 40763, previously reported to produce the metabolite strepturidin (STU). The isolated compounds were characterized by HRMS and spectroscopic techniques and they selectively inhibited transcription by bacterial RNA polymerase as previously reported for PUM. In contrast, STU could not be detected in the cultures of S. albus DSM 40763. As the reported characteristics reported for STU are almost identical with that of PUM, the existence of STU was questioned. We further sequenced the genome of S. albus DSM 40763 and identified a gene cluster that contains orthologs of all PUM biosynthesis enzymes but lacks the enzymes that would conceivably allow biosynthesis of STU as an additional product. Nature Publishing Group UK 2019-06-20 /pmc/articles/PMC6586884/ /pubmed/31222036 http://dx.doi.org/10.1038/s41598-019-45375-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Rosenqvist, Petja Palmu, Kaisa Prajapati, Ranjit Kumar Yamada, Keith Niemi, Jarmo Belogurov, Georgiy A. Metsä-Ketelä, Mikko Virta, Pasi Characterization of C-nucleoside Antimicrobials from Streptomyces albus DSM 40763: Strepturidin is Pseudouridimycin |
title | Characterization of C-nucleoside Antimicrobials from Streptomyces albus DSM 40763: Strepturidin is Pseudouridimycin |
title_full | Characterization of C-nucleoside Antimicrobials from Streptomyces albus DSM 40763: Strepturidin is Pseudouridimycin |
title_fullStr | Characterization of C-nucleoside Antimicrobials from Streptomyces albus DSM 40763: Strepturidin is Pseudouridimycin |
title_full_unstemmed | Characterization of C-nucleoside Antimicrobials from Streptomyces albus DSM 40763: Strepturidin is Pseudouridimycin |
title_short | Characterization of C-nucleoside Antimicrobials from Streptomyces albus DSM 40763: Strepturidin is Pseudouridimycin |
title_sort | characterization of c-nucleoside antimicrobials from streptomyces albus dsm 40763: strepturidin is pseudouridimycin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586884/ https://www.ncbi.nlm.nih.gov/pubmed/31222036 http://dx.doi.org/10.1038/s41598-019-45375-w |
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