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Design and fabrication of a hybrid alginate hydrogel/poly(ε‐caprolactone) mold for auricular cartilage reconstruction
The aim of this study was to design and manufacture an easily assembled cartilage implant model for auricular reconstruction. First, the printing accuracy and mechanical properties of 3D‐printed poly‐ε‐caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material pr...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587956/ https://www.ncbi.nlm.nih.gov/pubmed/30383916 http://dx.doi.org/10.1002/jbm.b.34264 |
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author | Visscher, D. O. Gleadall, A. Buskermolen, J. K. Burla, F. Segal, J. Koenderink, G. H. Helder, M. N. van Zuijlen, P. P. M. |
author_facet | Visscher, D. O. Gleadall, A. Buskermolen, J. K. Burla, F. Segal, J. Koenderink, G. H. Helder, M. N. van Zuijlen, P. P. M. |
author_sort | Visscher, D. O. |
collection | PubMed |
description | The aim of this study was to design and manufacture an easily assembled cartilage implant model for auricular reconstruction. First, the printing accuracy and mechanical properties of 3D‐printed poly‐ε‐caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neocartilage formation, and printing accuracy), a hybrid auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced cartilage‐like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p > .05). The final auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid auricular mold appears to be an adequate model for cartilage tissue engineering and may provide a novel approach to auricular cartilage regeneration for facial reconstruction. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1711–1721, 2019. |
format | Online Article Text |
id | pubmed-6587956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65879562019-07-02 Design and fabrication of a hybrid alginate hydrogel/poly(ε‐caprolactone) mold for auricular cartilage reconstruction Visscher, D. O. Gleadall, A. Buskermolen, J. K. Burla, F. Segal, J. Koenderink, G. H. Helder, M. N. van Zuijlen, P. P. M. J Biomed Mater Res B Appl Biomater Original Research Reports The aim of this study was to design and manufacture an easily assembled cartilage implant model for auricular reconstruction. First, the printing accuracy and mechanical properties of 3D‐printed poly‐ε‐caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neocartilage formation, and printing accuracy), a hybrid auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced cartilage‐like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p > .05). The final auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid auricular mold appears to be an adequate model for cartilage tissue engineering and may provide a novel approach to auricular cartilage regeneration for facial reconstruction. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1711–1721, 2019. John Wiley & Sons, Inc. 2018-11-01 2019-07 /pmc/articles/PMC6587956/ /pubmed/30383916 http://dx.doi.org/10.1002/jbm.b.34264 Text en © 2018 The Authors. JOURNAL of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Research Reports Visscher, D. O. Gleadall, A. Buskermolen, J. K. Burla, F. Segal, J. Koenderink, G. H. Helder, M. N. van Zuijlen, P. P. M. Design and fabrication of a hybrid alginate hydrogel/poly(ε‐caprolactone) mold for auricular cartilage reconstruction |
title | Design and fabrication of a hybrid alginate hydrogel/poly(ε‐caprolactone) mold for auricular cartilage reconstruction |
title_full | Design and fabrication of a hybrid alginate hydrogel/poly(ε‐caprolactone) mold for auricular cartilage reconstruction |
title_fullStr | Design and fabrication of a hybrid alginate hydrogel/poly(ε‐caprolactone) mold for auricular cartilage reconstruction |
title_full_unstemmed | Design and fabrication of a hybrid alginate hydrogel/poly(ε‐caprolactone) mold for auricular cartilage reconstruction |
title_short | Design and fabrication of a hybrid alginate hydrogel/poly(ε‐caprolactone) mold for auricular cartilage reconstruction |
title_sort | design and fabrication of a hybrid alginate hydrogel/poly(ε‐caprolactone) mold for auricular cartilage reconstruction |
topic | Original Research Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587956/ https://www.ncbi.nlm.nih.gov/pubmed/30383916 http://dx.doi.org/10.1002/jbm.b.34264 |
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