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Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells

Microneedles are promising devices for transdermal delivery and diagnostic applications, due to their minimally invasive and painless nature of application. However, so far, applications are limited to small scale research projects. Material selection and production for larger projects remain a chal...

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Autores principales: Schossleitner, Klaudia, O'Mahony, Conor, Brandstätter, Stefan, Haslinger, Michael J., Demuth, Sabrina, Fechtig, Daniel, Petzelbauer, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587957/
https://www.ncbi.nlm.nih.gov/pubmed/30456923
http://dx.doi.org/10.1002/jbm.a.36565
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author Schossleitner, Klaudia
O'Mahony, Conor
Brandstätter, Stefan
Haslinger, Michael J.
Demuth, Sabrina
Fechtig, Daniel
Petzelbauer, Peter
author_facet Schossleitner, Klaudia
O'Mahony, Conor
Brandstätter, Stefan
Haslinger, Michael J.
Demuth, Sabrina
Fechtig, Daniel
Petzelbauer, Peter
author_sort Schossleitner, Klaudia
collection PubMed
description Microneedles are promising devices for transdermal delivery and diagnostic applications, due to their minimally invasive and painless nature of application. However, so far, applications are limited to small scale research projects. Material selection and production for larger projects remain a challenge. In vitro testing using human cell culture could bridge the gap between cost effective screening of suitable materials and concerns for safety and ethics. In this study, materials were tested for effects on viability and morphology of human endothelial cells and keratinocytes. In addition, materials were assessed for their potential to influence cellular differentiation and barrier formation. Elution‐based testing of inflammatory markers revealed no negative effects in all applied tests, whereas the assessment of differentiation markers on cells in direct contact with the material showed differences and allowed the selection of candidate materials for future medical device applications. This study illustrates that elution‐based biocompatibility testing can paint an incomplete picture. Advanced staining techniques and cell types specific for the application of the medical device improve material selection to reduce and replace animal testing at an early stage in the development process. © 2018 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 505–512, 2019.
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spelling pubmed-65879572019-07-02 Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells Schossleitner, Klaudia O'Mahony, Conor Brandstätter, Stefan Haslinger, Michael J. Demuth, Sabrina Fechtig, Daniel Petzelbauer, Peter J Biomed Mater Res A Original Articles Microneedles are promising devices for transdermal delivery and diagnostic applications, due to their minimally invasive and painless nature of application. However, so far, applications are limited to small scale research projects. Material selection and production for larger projects remain a challenge. In vitro testing using human cell culture could bridge the gap between cost effective screening of suitable materials and concerns for safety and ethics. In this study, materials were tested for effects on viability and morphology of human endothelial cells and keratinocytes. In addition, materials were assessed for their potential to influence cellular differentiation and barrier formation. Elution‐based testing of inflammatory markers revealed no negative effects in all applied tests, whereas the assessment of differentiation markers on cells in direct contact with the material showed differences and allowed the selection of candidate materials for future medical device applications. This study illustrates that elution‐based biocompatibility testing can paint an incomplete picture. Advanced staining techniques and cell types specific for the application of the medical device improve material selection to reduce and replace animal testing at an early stage in the development process. © 2018 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 505–512, 2019. John Wiley & Sons, Inc. 2018-11-19 2019-03 /pmc/articles/PMC6587957/ /pubmed/30456923 http://dx.doi.org/10.1002/jbm.a.36565 Text en © 2018 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Schossleitner, Klaudia
O'Mahony, Conor
Brandstätter, Stefan
Haslinger, Michael J.
Demuth, Sabrina
Fechtig, Daniel
Petzelbauer, Peter
Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells
title Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells
title_full Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells
title_fullStr Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells
title_full_unstemmed Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells
title_short Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells
title_sort differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587957/
https://www.ncbi.nlm.nih.gov/pubmed/30456923
http://dx.doi.org/10.1002/jbm.a.36565
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