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Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis

Canine Atopic Dermatitis (AD) is a common complex and multifactorial disease involving immune dysregulation, genetic predisposition, skin barrier defects, environmental factors and allergic sensitization. To date, diagnosis of canine AD relies on a combination of patient history, clinical examinatio...

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Autores principales: Koury, Jeffrey, Ramirez, Ana, Xie, Chen, Harb, Jerry, Dong, Charli, Maki, Chad, Ramos, Tom, Izadyar, Fari, Clark, David, Drechsler, Yvonne, Kaur, Gagandeep, Hao, Jijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588236/
https://www.ncbi.nlm.nih.gov/pubmed/31226136
http://dx.doi.org/10.1371/journal.pone.0218670
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author Koury, Jeffrey
Ramirez, Ana
Xie, Chen
Harb, Jerry
Dong, Charli
Maki, Chad
Ramos, Tom
Izadyar, Fari
Clark, David
Drechsler, Yvonne
Kaur, Gagandeep
Hao, Jijun
author_facet Koury, Jeffrey
Ramirez, Ana
Xie, Chen
Harb, Jerry
Dong, Charli
Maki, Chad
Ramos, Tom
Izadyar, Fari
Clark, David
Drechsler, Yvonne
Kaur, Gagandeep
Hao, Jijun
author_sort Koury, Jeffrey
collection PubMed
description Canine Atopic Dermatitis (AD) is a common complex and multifactorial disease involving immune dysregulation, genetic predisposition, skin barrier defects, environmental factors and allergic sensitization. To date, diagnosis of canine AD relies on a combination of patient history, clinical examination, allergy testing and response to diet trials/therapies with no reliable biomarkers available to distinguish AD from other diseases with similar clinical presentations. A handful of studies to identify potential biomarkers in the peripheral blood of AD dogs and healthy controls have been performed with some showing inconsistent and contradictory results. In this study, we, for the first time, report statistically significant increases in expression of phosphodiesterase 4D (PDE4D) gene in peripheral blood mononuclear cells (PBMCs) and miR-203 in plasma from AD dogs compared to healthy controls. In addition, we report a statistically non-significant change of the CD4(+)/CD8(+) ratio, a dramatic decrease of three gene markers (PIAS1, RORA and SH2B1) as well as a panel of differential expression of cytokines in AD dogs in comparison to the healthy controls. Our study provides important insight into the complexities of canine AD, and further studies to verify the specificity of these findings for canine AD at a larger-scale are warranted.
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spelling pubmed-65882362019-06-28 Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis Koury, Jeffrey Ramirez, Ana Xie, Chen Harb, Jerry Dong, Charli Maki, Chad Ramos, Tom Izadyar, Fari Clark, David Drechsler, Yvonne Kaur, Gagandeep Hao, Jijun PLoS One Research Article Canine Atopic Dermatitis (AD) is a common complex and multifactorial disease involving immune dysregulation, genetic predisposition, skin barrier defects, environmental factors and allergic sensitization. To date, diagnosis of canine AD relies on a combination of patient history, clinical examination, allergy testing and response to diet trials/therapies with no reliable biomarkers available to distinguish AD from other diseases with similar clinical presentations. A handful of studies to identify potential biomarkers in the peripheral blood of AD dogs and healthy controls have been performed with some showing inconsistent and contradictory results. In this study, we, for the first time, report statistically significant increases in expression of phosphodiesterase 4D (PDE4D) gene in peripheral blood mononuclear cells (PBMCs) and miR-203 in plasma from AD dogs compared to healthy controls. In addition, we report a statistically non-significant change of the CD4(+)/CD8(+) ratio, a dramatic decrease of three gene markers (PIAS1, RORA and SH2B1) as well as a panel of differential expression of cytokines in AD dogs in comparison to the healthy controls. Our study provides important insight into the complexities of canine AD, and further studies to verify the specificity of these findings for canine AD at a larger-scale are warranted. Public Library of Science 2019-06-21 /pmc/articles/PMC6588236/ /pubmed/31226136 http://dx.doi.org/10.1371/journal.pone.0218670 Text en © 2019 Koury et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Koury, Jeffrey
Ramirez, Ana
Xie, Chen
Harb, Jerry
Dong, Charli
Maki, Chad
Ramos, Tom
Izadyar, Fari
Clark, David
Drechsler, Yvonne
Kaur, Gagandeep
Hao, Jijun
Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis
title Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis
title_full Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis
title_fullStr Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis
title_full_unstemmed Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis
title_short Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis
title_sort phosphodiesterase 4d, mir-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588236/
https://www.ncbi.nlm.nih.gov/pubmed/31226136
http://dx.doi.org/10.1371/journal.pone.0218670
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