Cargando…
Archaeal community variation in the Qinhuangdao coastal aquaculture zone revealed by high-throughput sequencing
The differences in archaeal diversity and community composition in the sediments and waters of the Qinhuangdao coastal aquaculture zone were investigated. Furthermore, the associations between dominant archaeal taxa with geographic and environmental variables were evaluated. High-throughput sequenci...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588238/ https://www.ncbi.nlm.nih.gov/pubmed/31226149 http://dx.doi.org/10.1371/journal.pone.0218611 |
_version_ | 1783429215151980544 |
---|---|
author | Wang, Shuping Zheng, Xin Xia, Huijuan Shi, Di Fan, Juntao Wang, Pengyuan Yan, Zhenguang |
author_facet | Wang, Shuping Zheng, Xin Xia, Huijuan Shi, Di Fan, Juntao Wang, Pengyuan Yan, Zhenguang |
author_sort | Wang, Shuping |
collection | PubMed |
description | The differences in archaeal diversity and community composition in the sediments and waters of the Qinhuangdao coastal aquaculture zone were investigated. Furthermore, the associations between dominant archaeal taxa with geographic and environmental variables were evaluated. High-throughput sequencing of archaeal 16S rRNA genes yielded a total of 176,211 quality-filtered reads and 1,178 operational taxonomic units (OTUs) overall. The most abundant phylum and class among all communities were Thaumarchaeota and Nitrososphaeria, respectively. Beta diversity analysis indicated that community composition was divided into two groups according to the habitat type (i.e., sediments or waters). Only 9.8% OTUs were shared by communities from the two habitats, while 73.9% and 16.3% of the OTUs were unique to sediment or water communities, respectively. Furthermore, the relative abundances of the dominant OTUs differed with habitat type. Investigations of relationships between dominant OTUs and environmental variables indicated that some dominant OTUs were more sensitive to variation in environmental factors, which could be due to individual taxonomic differences in lifestyles and biological processes. Overall, the investigation of archaeal community variation within the Qinhuangdao coastal aquaculture zone provides an important baseline understanding of the microbial ecology in this important ecosystem. |
format | Online Article Text |
id | pubmed-6588238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65882382019-06-28 Archaeal community variation in the Qinhuangdao coastal aquaculture zone revealed by high-throughput sequencing Wang, Shuping Zheng, Xin Xia, Huijuan Shi, Di Fan, Juntao Wang, Pengyuan Yan, Zhenguang PLoS One Research Article The differences in archaeal diversity and community composition in the sediments and waters of the Qinhuangdao coastal aquaculture zone were investigated. Furthermore, the associations between dominant archaeal taxa with geographic and environmental variables were evaluated. High-throughput sequencing of archaeal 16S rRNA genes yielded a total of 176,211 quality-filtered reads and 1,178 operational taxonomic units (OTUs) overall. The most abundant phylum and class among all communities were Thaumarchaeota and Nitrososphaeria, respectively. Beta diversity analysis indicated that community composition was divided into two groups according to the habitat type (i.e., sediments or waters). Only 9.8% OTUs were shared by communities from the two habitats, while 73.9% and 16.3% of the OTUs were unique to sediment or water communities, respectively. Furthermore, the relative abundances of the dominant OTUs differed with habitat type. Investigations of relationships between dominant OTUs and environmental variables indicated that some dominant OTUs were more sensitive to variation in environmental factors, which could be due to individual taxonomic differences in lifestyles and biological processes. Overall, the investigation of archaeal community variation within the Qinhuangdao coastal aquaculture zone provides an important baseline understanding of the microbial ecology in this important ecosystem. Public Library of Science 2019-06-21 /pmc/articles/PMC6588238/ /pubmed/31226149 http://dx.doi.org/10.1371/journal.pone.0218611 Text en © 2019 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Shuping Zheng, Xin Xia, Huijuan Shi, Di Fan, Juntao Wang, Pengyuan Yan, Zhenguang Archaeal community variation in the Qinhuangdao coastal aquaculture zone revealed by high-throughput sequencing |
title | Archaeal community variation in the Qinhuangdao coastal aquaculture zone revealed by high-throughput sequencing |
title_full | Archaeal community variation in the Qinhuangdao coastal aquaculture zone revealed by high-throughput sequencing |
title_fullStr | Archaeal community variation in the Qinhuangdao coastal aquaculture zone revealed by high-throughput sequencing |
title_full_unstemmed | Archaeal community variation in the Qinhuangdao coastal aquaculture zone revealed by high-throughput sequencing |
title_short | Archaeal community variation in the Qinhuangdao coastal aquaculture zone revealed by high-throughput sequencing |
title_sort | archaeal community variation in the qinhuangdao coastal aquaculture zone revealed by high-throughput sequencing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588238/ https://www.ncbi.nlm.nih.gov/pubmed/31226149 http://dx.doi.org/10.1371/journal.pone.0218611 |
work_keys_str_mv | AT wangshuping archaealcommunityvariationintheqinhuangdaocoastalaquaculturezonerevealedbyhighthroughputsequencing AT zhengxin archaealcommunityvariationintheqinhuangdaocoastalaquaculturezonerevealedbyhighthroughputsequencing AT xiahuijuan archaealcommunityvariationintheqinhuangdaocoastalaquaculturezonerevealedbyhighthroughputsequencing AT shidi archaealcommunityvariationintheqinhuangdaocoastalaquaculturezonerevealedbyhighthroughputsequencing AT fanjuntao archaealcommunityvariationintheqinhuangdaocoastalaquaculturezonerevealedbyhighthroughputsequencing AT wangpengyuan archaealcommunityvariationintheqinhuangdaocoastalaquaculturezonerevealedbyhighthroughputsequencing AT yanzhenguang archaealcommunityvariationintheqinhuangdaocoastalaquaculturezonerevealedbyhighthroughputsequencing |