Cargando…

Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice

The increased prevalence of obesity and associated insulin resistance calls for effective therapeutic treatment of metabolic diseases. The current PPARγ-targeting antidiabetic drugs have undesirable side effects. The present study investigated the anti-diabetic and anti-obesity effects of withaferin...

Descripción completa

Detalles Bibliográficos
Autores principales: Khalilpourfarshbafi, Manizheh, Devi Murugan, Dharmani, Abdul Sattar, Munavvar Zubaid, Sucedaram, Yamuna, Abdullah, Nor Azizan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588247/
https://www.ncbi.nlm.nih.gov/pubmed/31226166
http://dx.doi.org/10.1371/journal.pone.0218792
_version_ 1783429217309949952
author Khalilpourfarshbafi, Manizheh
Devi Murugan, Dharmani
Abdul Sattar, Munavvar Zubaid
Sucedaram, Yamuna
Abdullah, Nor Azizan
author_facet Khalilpourfarshbafi, Manizheh
Devi Murugan, Dharmani
Abdul Sattar, Munavvar Zubaid
Sucedaram, Yamuna
Abdullah, Nor Azizan
author_sort Khalilpourfarshbafi, Manizheh
collection PubMed
description The increased prevalence of obesity and associated insulin resistance calls for effective therapeutic treatment of metabolic diseases. The current PPARγ-targeting antidiabetic drugs have undesirable side effects. The present study investigated the anti-diabetic and anti-obesity effects of withaferin A (WFA) in diet-induced obese (DIO) C57BL/6J mice and also the anti-adipogenic effect of WFA in differentiating 3T3- F442A cells. DIO mice were treated with WFA (6 mg/kg) or rosiglitazone (10 mg/kg) for 8 weeks. At the end of the treatment period, metabolic profile, liver function and inflammatory parameters were obtained. Expression of selective genes controlling insulin signaling, inflammation, adipogenesis, energy expenditure and PPARγ phosphorylation-regulated genes in epididymal fats were analyzed. Furthermore, the anti-adipogenic effect of WFA was evaluated in 3T3- F442A cell line. WFA treatment prevented weight gain without affecting food or caloric intake in DIO mice. WFA-treated group also exhibited lower epididymal and mesenteric fat pad mass, an improvement in lipid profile and hepatic steatosis and a reduction in serum inflammatory cytokines. Insulin resistance was reduced as shown by an improvement in glucose and insulin tolerance and serum adiponectin. WFA treatment upregulated selective insulin signaling (insr, irs1, slc2a4 and pi3k) and PPARγ phosphorylation-regulated (car3, selenbp1, aplp2, txnip, and adipoq) genes, downregulated inflammatory (tnf-α and il-6) genes and altered energy expenditure controlling (tph2 and adrb3) genes. In 3T3- F442A cell line, withaferin A inhibited adipogenesis as indicated by a decrease in lipid accumulation in differentiating adipocytes and protein expression of PPARγ and C/EBPα. The effect of rosiglitazone on physiological and lipid profiles, insulin resistance, some genes expression and differentiating adipocytes were markedly different. Our data suggest that WFA is a promising therapeutic agent for both diabetes and obesity.
format Online
Article
Text
id pubmed-6588247
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-65882472019-06-28 Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice Khalilpourfarshbafi, Manizheh Devi Murugan, Dharmani Abdul Sattar, Munavvar Zubaid Sucedaram, Yamuna Abdullah, Nor Azizan PLoS One Research Article The increased prevalence of obesity and associated insulin resistance calls for effective therapeutic treatment of metabolic diseases. The current PPARγ-targeting antidiabetic drugs have undesirable side effects. The present study investigated the anti-diabetic and anti-obesity effects of withaferin A (WFA) in diet-induced obese (DIO) C57BL/6J mice and also the anti-adipogenic effect of WFA in differentiating 3T3- F442A cells. DIO mice were treated with WFA (6 mg/kg) or rosiglitazone (10 mg/kg) for 8 weeks. At the end of the treatment period, metabolic profile, liver function and inflammatory parameters were obtained. Expression of selective genes controlling insulin signaling, inflammation, adipogenesis, energy expenditure and PPARγ phosphorylation-regulated genes in epididymal fats were analyzed. Furthermore, the anti-adipogenic effect of WFA was evaluated in 3T3- F442A cell line. WFA treatment prevented weight gain without affecting food or caloric intake in DIO mice. WFA-treated group also exhibited lower epididymal and mesenteric fat pad mass, an improvement in lipid profile and hepatic steatosis and a reduction in serum inflammatory cytokines. Insulin resistance was reduced as shown by an improvement in glucose and insulin tolerance and serum adiponectin. WFA treatment upregulated selective insulin signaling (insr, irs1, slc2a4 and pi3k) and PPARγ phosphorylation-regulated (car3, selenbp1, aplp2, txnip, and adipoq) genes, downregulated inflammatory (tnf-α and il-6) genes and altered energy expenditure controlling (tph2 and adrb3) genes. In 3T3- F442A cell line, withaferin A inhibited adipogenesis as indicated by a decrease in lipid accumulation in differentiating adipocytes and protein expression of PPARγ and C/EBPα. The effect of rosiglitazone on physiological and lipid profiles, insulin resistance, some genes expression and differentiating adipocytes were markedly different. Our data suggest that WFA is a promising therapeutic agent for both diabetes and obesity. Public Library of Science 2019-06-21 /pmc/articles/PMC6588247/ /pubmed/31226166 http://dx.doi.org/10.1371/journal.pone.0218792 Text en © 2019 Khalilpourfarshbafi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Khalilpourfarshbafi, Manizheh
Devi Murugan, Dharmani
Abdul Sattar, Munavvar Zubaid
Sucedaram, Yamuna
Abdullah, Nor Azizan
Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice
title Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice
title_full Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice
title_fullStr Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice
title_full_unstemmed Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice
title_short Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice
title_sort withaferin a inhibits adipogenesis in 3t3-f442a cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588247/
https://www.ncbi.nlm.nih.gov/pubmed/31226166
http://dx.doi.org/10.1371/journal.pone.0218792
work_keys_str_mv AT khalilpourfarshbafimanizheh withaferinainhibitsadipogenesisin3t3f442acelllineimprovesinsulinsensitivityandpromotesweightlossinhighfatdietinducedobesemice
AT devimurugandharmani withaferinainhibitsadipogenesisin3t3f442acelllineimprovesinsulinsensitivityandpromotesweightlossinhighfatdietinducedobesemice
AT abdulsattarmunavvarzubaid withaferinainhibitsadipogenesisin3t3f442acelllineimprovesinsulinsensitivityandpromotesweightlossinhighfatdietinducedobesemice
AT sucedaramyamuna withaferinainhibitsadipogenesisin3t3f442acelllineimprovesinsulinsensitivityandpromotesweightlossinhighfatdietinducedobesemice
AT abdullahnorazizan withaferinainhibitsadipogenesisin3t3f442acelllineimprovesinsulinsensitivityandpromotesweightlossinhighfatdietinducedobesemice