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Development of extraction and detection method for fluridone in water and sediment by HPLC-UV

Fluridone is widely used as a herbicide for controlling invasive aquatic plants such as hydrilla in surface water bodies. When applied on surface waters fluridone can attach to bed sediment, requiring rigorous extraction methods prior to analysis. Currently, very limited information exists in terms...

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Autores principales: Wickham, Patrick, Singh, Latika, Pandey, Pramod, Lesmeister, Sarah, Gilbert, Patricia, Kwong, Michael, Caudill, Jeffrey, O’Brien, Jon, Biswas, Sagor, Teh, Swee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588664/
https://www.ncbi.nlm.nih.gov/pubmed/31227931
http://dx.doi.org/10.1186/s13568-019-0807-4
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author Wickham, Patrick
Singh, Latika
Pandey, Pramod
Lesmeister, Sarah
Gilbert, Patricia
Kwong, Michael
Caudill, Jeffrey
O’Brien, Jon
Biswas, Sagor
Teh, Swee
author_facet Wickham, Patrick
Singh, Latika
Pandey, Pramod
Lesmeister, Sarah
Gilbert, Patricia
Kwong, Michael
Caudill, Jeffrey
O’Brien, Jon
Biswas, Sagor
Teh, Swee
author_sort Wickham, Patrick
collection PubMed
description Fluridone is widely used as a herbicide for controlling invasive aquatic plants such as hydrilla in surface water bodies. When applied on surface waters fluridone can attach to bed sediment, requiring rigorous extraction methods prior to analysis. Currently, very limited information exists in terms of fluridone residue detection in delta sediment. In this study, we researched fluridone detection in both water and sediment. To extract fluridone from sediment, here we have tested two extraction methods: (1) a rotavapor method (RM); and (2) a quick, easy, cheap, effective, rugged and safe (QuEChERS) method (QM). The extraction results of RM were compared with those of QM. To quantify fluridone concentrations in extracts, a high-performance liquid chromatography (HPLC)-UV detector was used. HPLC separation was achieved using an Allure C18 5 µm 150 × 4.6 mm column with a mobile phase composed of acetonitrile and water (60:40, v/v). The UV detector was operated at 237 nm. The method was tested and validated using a series of water and sediment samples taken from Sacramento–San Joaquin Delta in California. The average recovery of fluridone was 73% and 78% using RM and QM respectively. The proposed method can be used for testing fluridone in water and sediment samples.
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spelling pubmed-65886642019-07-05 Development of extraction and detection method for fluridone in water and sediment by HPLC-UV Wickham, Patrick Singh, Latika Pandey, Pramod Lesmeister, Sarah Gilbert, Patricia Kwong, Michael Caudill, Jeffrey O’Brien, Jon Biswas, Sagor Teh, Swee AMB Express Original Article Fluridone is widely used as a herbicide for controlling invasive aquatic plants such as hydrilla in surface water bodies. When applied on surface waters fluridone can attach to bed sediment, requiring rigorous extraction methods prior to analysis. Currently, very limited information exists in terms of fluridone residue detection in delta sediment. In this study, we researched fluridone detection in both water and sediment. To extract fluridone from sediment, here we have tested two extraction methods: (1) a rotavapor method (RM); and (2) a quick, easy, cheap, effective, rugged and safe (QuEChERS) method (QM). The extraction results of RM were compared with those of QM. To quantify fluridone concentrations in extracts, a high-performance liquid chromatography (HPLC)-UV detector was used. HPLC separation was achieved using an Allure C18 5 µm 150 × 4.6 mm column with a mobile phase composed of acetonitrile and water (60:40, v/v). The UV detector was operated at 237 nm. The method was tested and validated using a series of water and sediment samples taken from Sacramento–San Joaquin Delta in California. The average recovery of fluridone was 73% and 78% using RM and QM respectively. The proposed method can be used for testing fluridone in water and sediment samples. Springer Berlin Heidelberg 2019-06-21 /pmc/articles/PMC6588664/ /pubmed/31227931 http://dx.doi.org/10.1186/s13568-019-0807-4 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Wickham, Patrick
Singh, Latika
Pandey, Pramod
Lesmeister, Sarah
Gilbert, Patricia
Kwong, Michael
Caudill, Jeffrey
O’Brien, Jon
Biswas, Sagor
Teh, Swee
Development of extraction and detection method for fluridone in water and sediment by HPLC-UV
title Development of extraction and detection method for fluridone in water and sediment by HPLC-UV
title_full Development of extraction and detection method for fluridone in water and sediment by HPLC-UV
title_fullStr Development of extraction and detection method for fluridone in water and sediment by HPLC-UV
title_full_unstemmed Development of extraction and detection method for fluridone in water and sediment by HPLC-UV
title_short Development of extraction and detection method for fluridone in water and sediment by HPLC-UV
title_sort development of extraction and detection method for fluridone in water and sediment by hplc-uv
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588664/
https://www.ncbi.nlm.nih.gov/pubmed/31227931
http://dx.doi.org/10.1186/s13568-019-0807-4
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