Comprehensive assessment of multiple biases in small RNA sequencing reveals significant differences in the performance of widely used methods

BACKGROUND: RNA sequencing offers advantages over other quantification methods for microRNA (miRNA), yet numerous biases make reliable quantification challenging. Previous evaluations of these biases have focused on adapter ligation bias with limited evaluation of reverse transcription bias or ampli...

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Detalles Bibliográficos
Autores principales: Wright, Carrie, Rajpurohit, Anandita, Burke, Emily E., Williams, Courtney, Collado-Torres, Leonardo, Kimos, Martha, Brandon, Nicholas J., Cross, Alan J., Jaffe, Andrew E., Weinberger, Daniel R., Shin, Joo Heon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588940/
https://www.ncbi.nlm.nih.gov/pubmed/31226924
http://dx.doi.org/10.1186/s12864-019-5870-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588940/
https://www.ncbi.nlm.nih.gov/pubmed/31226924
http://dx.doi.org/10.1186/s12864-019-5870-3

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