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Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations
Interactions between different cell types can induce distinct contact inhibition of locomotion (CIL) responses that are hypothesised to control population-wide behaviours during embryogenesis. However, our understanding of the signals that lead to cell-type specific repulsion and the precise capacit...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589087/ https://www.ncbi.nlm.nih.gov/pubmed/31076510 http://dx.doi.org/10.1242/jcs.223974 |
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author | Brayford, Simon Kenny, Fiona N. Hiratsuka, Toru Serna-Morales, Eduardo Yolland, Lawrence Luchici, Andrei Stramer, Brian M. |
author_facet | Brayford, Simon Kenny, Fiona N. Hiratsuka, Toru Serna-Morales, Eduardo Yolland, Lawrence Luchici, Andrei Stramer, Brian M. |
author_sort | Brayford, Simon |
collection | PubMed |
description | Interactions between different cell types can induce distinct contact inhibition of locomotion (CIL) responses that are hypothesised to control population-wide behaviours during embryogenesis. However, our understanding of the signals that lead to cell-type specific repulsion and the precise capacity of heterotypic CIL responses to drive emergent behaviours is lacking. Using a new model of heterotypic CIL, we show that fibrosarcoma cells, but not fibroblasts, are actively repelled by epithelial cells in culture. We show that knocking down EphB2 or ERK in fibrosarcoma cells specifically leads to disruption of the repulsion phase of CIL in response to interactions with epithelial cells. We also examine the population-wide effects when these various cell combinations are allowed to interact in culture. Unlike fibroblasts, fibrosarcoma cells completely segregate from epithelial cells and inhibiting their distinct CIL response by knocking down EphB2 or ERK family proteins also disrupts this emergent sorting behaviour. These data suggest that heterotypic CIL responses, in conjunction with processes such as differential adhesion, may aid the sorting of cell populations. |
format | Online Article Text |
id | pubmed-6589087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65890872019-07-16 Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations Brayford, Simon Kenny, Fiona N. Hiratsuka, Toru Serna-Morales, Eduardo Yolland, Lawrence Luchici, Andrei Stramer, Brian M. J Cell Sci Short Report Interactions between different cell types can induce distinct contact inhibition of locomotion (CIL) responses that are hypothesised to control population-wide behaviours during embryogenesis. However, our understanding of the signals that lead to cell-type specific repulsion and the precise capacity of heterotypic CIL responses to drive emergent behaviours is lacking. Using a new model of heterotypic CIL, we show that fibrosarcoma cells, but not fibroblasts, are actively repelled by epithelial cells in culture. We show that knocking down EphB2 or ERK in fibrosarcoma cells specifically leads to disruption of the repulsion phase of CIL in response to interactions with epithelial cells. We also examine the population-wide effects when these various cell combinations are allowed to interact in culture. Unlike fibroblasts, fibrosarcoma cells completely segregate from epithelial cells and inhibiting their distinct CIL response by knocking down EphB2 or ERK family proteins also disrupts this emergent sorting behaviour. These data suggest that heterotypic CIL responses, in conjunction with processes such as differential adhesion, may aid the sorting of cell populations. The Company of Biologists Ltd 2019-06-01 2019-05-31 /pmc/articles/PMC6589087/ /pubmed/31076510 http://dx.doi.org/10.1242/jcs.223974 Text en © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Short Report Brayford, Simon Kenny, Fiona N. Hiratsuka, Toru Serna-Morales, Eduardo Yolland, Lawrence Luchici, Andrei Stramer, Brian M. Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations |
title | Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations |
title_full | Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations |
title_fullStr | Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations |
title_full_unstemmed | Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations |
title_short | Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations |
title_sort | heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589087/ https://www.ncbi.nlm.nih.gov/pubmed/31076510 http://dx.doi.org/10.1242/jcs.223974 |
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