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Actomyosin contractility modulates Wnt signaling through adherens junction stability

Actomyosin contractility can influence the canonical Wnt signaling pathway in processes like mesoderm differentiation and tissue stiffness during tumorigenesis. We identified that increased nonmuscle myosin II activation and cellular contraction inhibited Wnt target gene transcription in developing...

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Detalles Bibliográficos
Autores principales: Hall, Eric T., Hoesing, Elizabeth, Sinkovics, Endre, Verheyen, Esther M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589568/
https://www.ncbi.nlm.nih.gov/pubmed/30540525
http://dx.doi.org/10.1091/mbc.E18-06-0345
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author Hall, Eric T.
Hoesing, Elizabeth
Sinkovics, Endre
Verheyen, Esther M.
author_facet Hall, Eric T.
Hoesing, Elizabeth
Sinkovics, Endre
Verheyen, Esther M.
author_sort Hall, Eric T.
collection PubMed
description Actomyosin contractility can influence the canonical Wnt signaling pathway in processes like mesoderm differentiation and tissue stiffness during tumorigenesis. We identified that increased nonmuscle myosin II activation and cellular contraction inhibited Wnt target gene transcription in developing Drosophila imaginal disks. Genetic interactions studies were used to show that this effect was due to myosin-induced accumulation of cortical F-actin resulting in clustering and accumulation of E-cadherin to the adherens junctions. This results in E-cadherin titrating any available β-catenin, the Wnt pathway transcriptional coactivator, to the adherens junctions in order to maintain cell–cell adhesion under contraction. We show that decreased levels of cytoplasmic β-catenin result in insufficient nuclear translocation for full Wnt target gene transcription. Previous studies have identified some of these interactions, but we present a thorough analysis using the wing disk epithelium to show the consequences of modulating myosin phosphatase. Our work elucidates a mechanism in which the dynamic promotion of actomyosin contractility refines patterning of Wnt transcription during development and maintenance of epithelial tissue in organisms.
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spelling pubmed-65895682019-06-28 Actomyosin contractility modulates Wnt signaling through adherens junction stability Hall, Eric T. Hoesing, Elizabeth Sinkovics, Endre Verheyen, Esther M. Mol Biol Cell Articles Actomyosin contractility can influence the canonical Wnt signaling pathway in processes like mesoderm differentiation and tissue stiffness during tumorigenesis. We identified that increased nonmuscle myosin II activation and cellular contraction inhibited Wnt target gene transcription in developing Drosophila imaginal disks. Genetic interactions studies were used to show that this effect was due to myosin-induced accumulation of cortical F-actin resulting in clustering and accumulation of E-cadherin to the adherens junctions. This results in E-cadherin titrating any available β-catenin, the Wnt pathway transcriptional coactivator, to the adherens junctions in order to maintain cell–cell adhesion under contraction. We show that decreased levels of cytoplasmic β-catenin result in insufficient nuclear translocation for full Wnt target gene transcription. Previous studies have identified some of these interactions, but we present a thorough analysis using the wing disk epithelium to show the consequences of modulating myosin phosphatase. Our work elucidates a mechanism in which the dynamic promotion of actomyosin contractility refines patterning of Wnt transcription during development and maintenance of epithelial tissue in organisms. The American Society for Cell Biology 2019-02-01 /pmc/articles/PMC6589568/ /pubmed/30540525 http://dx.doi.org/10.1091/mbc.E18-06-0345 Text en © 2019 Hall et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Hall, Eric T.
Hoesing, Elizabeth
Sinkovics, Endre
Verheyen, Esther M.
Actomyosin contractility modulates Wnt signaling through adherens junction stability
title Actomyosin contractility modulates Wnt signaling through adherens junction stability
title_full Actomyosin contractility modulates Wnt signaling through adherens junction stability
title_fullStr Actomyosin contractility modulates Wnt signaling through adherens junction stability
title_full_unstemmed Actomyosin contractility modulates Wnt signaling through adherens junction stability
title_short Actomyosin contractility modulates Wnt signaling through adherens junction stability
title_sort actomyosin contractility modulates wnt signaling through adherens junction stability
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589568/
https://www.ncbi.nlm.nih.gov/pubmed/30540525
http://dx.doi.org/10.1091/mbc.E18-06-0345
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